miMeasure – a standard for miRNA binding site characterization in mammalian cells

miMeasure – a standard for miRNA binding site characterization in mammalian cells

This RFC proposes a standard for the quantitative characterization of miRNA binding sites (miRNA-BS) in mammalian cells. The miMeasure standard introduces a ready-to-use standard measurement plasmid (pSMB_miMeasure, BBa_K337049) enabling rapid experimental characterization of any miRNA-BS of choice....

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Bibliographic Details
Main Authors: Adlung, Lorenz, Sabah, Jude Al, Bayer, Philipp, Berrens, Rebecca, Cristiano, Elena, Flocke, Lea, Kleinsorg, Stefan, Kolodziejczyk, Aleksandra, Kraemer, Stephen, Torre, Alejandro Macias, Mathur, Aastha, Mayilo, Dmytro, Neumann, Stefan, Niopek, Dominik, Pisa, Rudolf, Schad, Jan-Ulrich, Schumacher, Laura-Nadine, Uhlig, Thomas, Wu, Xiaoting, Keienburg, Jens, Boerner, Kathleen, Grimm, Dirk, Eils, Roland
Format: Technical Report
Language:English
Published: 2010
Subjects:
measurement standard
Online Access:http://hdl.handle.net/1721.1/60092
Description
Summary:This RFC proposes a standard for the quantitative characterization of miRNA binding sites (miRNA-BS) in mammalian cells. The miMeasure standard introduces a ready-to-use standard measurement plasmid (pSMB_miMeasure, BBa_K337049) enabling rapid experimental characterization of any miRNA-BS of choice. We recommend a new standard unit, RKDU (relative knock-down unit) to describe the knock-down efficiency of a miRNA-BS in a specific cell type. pSMB_miMeasure allows for an easy and fast measurement of RKDU while providing effective normalization against variance stemming from differences in transfection efficiency and from other sources.

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