On-chip whole-animal manipulation for high-throughput subcellular-resolution in-vivo drug/genetic screening
Techniques for rapid and automated small-animal manipulation and immobilization are necessary for high-throughput in vivo genetic/drug screens using cellular and sub-cellular features in multicellular organisms. We present a suite of technologies for complex high-throughput whole-animal genetic and...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | en_US |
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Institute of Electrical and Electronics Engineers
2010
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| Online Access: | http://hdl.handle.net/1721.1/60276 https://orcid.org/0000-0002-4612-1962 |
| _version_ | 1826200295243251712 |
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| author | Yanik, Mehmet Fatih Rohde, Christopher Benjamin Gilleland, Cody Lee Samara, Chrysanthi |
| author2 | Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science |
| author_facet | Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Yanik, Mehmet Fatih Rohde, Christopher Benjamin Gilleland, Cody Lee Samara, Chrysanthi |
| author_sort | Yanik, Mehmet Fatih |
| collection | MIT |
| description | Techniques for rapid and automated small-animal manipulation and immobilization are necessary for high-throughput in vivo genetic/drug screens using cellular and sub-cellular features in multicellular organisms. We present a suite of technologies for complex high-throughput whole-animal genetic and drug screens. We demonstrate a high-speed microfluidic sorter that can isolate and immobilize 'Caenorhabditis elegans' in a well-defined geometry, an integrated chip containing individually addressable screening chambers for incubation and exposure of individual animals to biochemical compounds, and a device for delivery of compound libraries in standard multiwell plates to microfluidic devices. The immobilization stability obtained by these devices is comparable to that of chemical anesthesia and the immobilization process does not affect lifespan, progeny production, or other aspects of animal health. The high-stability enables the use of a variety of key optical techniques. We use this to demonstrate femtosecond-laser nanosurgery and three-dimensional multiphoton microscopy to study neural regeneration at sub-cellular resolution in vivo. |
| first_indexed | 2024-09-23T11:34:36Z |
| format | Article |
| id | mit-1721.1/60276 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T11:34:36Z |
| publishDate | 2010 |
| publisher | Institute of Electrical and Electronics Engineers |
| record_format | dspace |
| spelling | mit-1721.1/602762022-09-27T20:26:02Z On-chip whole-animal manipulation for high-throughput subcellular-resolution in-vivo drug/genetic screening Yanik, Mehmet Fatih Rohde, Christopher Benjamin Gilleland, Cody Lee Samara, Chrysanthi Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Massachusetts Institute of Technology. Research Laboratory of Electronics Yanik, Mehmet Fatih Yanik, Mehmet Fatih Rohde, Christopher Benjamin Gilleland, Cody Lee Samara, Chrysanthi Techniques for rapid and automated small-animal manipulation and immobilization are necessary for high-throughput in vivo genetic/drug screens using cellular and sub-cellular features in multicellular organisms. We present a suite of technologies for complex high-throughput whole-animal genetic and drug screens. We demonstrate a high-speed microfluidic sorter that can isolate and immobilize 'Caenorhabditis elegans' in a well-defined geometry, an integrated chip containing individually addressable screening chambers for incubation and exposure of individual animals to biochemical compounds, and a device for delivery of compound libraries in standard multiwell plates to microfluidic devices. The immobilization stability obtained by these devices is comparable to that of chemical anesthesia and the immobilization process does not affect lifespan, progeny production, or other aspects of animal health. The high-stability enables the use of a variety of key optical techniques. We use this to demonstrate femtosecond-laser nanosurgery and three-dimensional multiphoton microscopy to study neural regeneration at sub-cellular resolution in vivo. National Institutes of Health.(U.S.) (Director’s New Innovator Award Program (1-DP2-OD002989–01) part of NIH Roadmap for Medical) 2010-12-10T21:18:02Z 2010-12-10T21:18:02Z 2009-05 2009-04 Article http://purl.org/eprint/type/ConferencePaper 978-1-4244-4292-8 INSPEC Accession Number: 10615852 http://hdl.handle.net/1721.1/60276 Rohde, C.B. et al. “On-chip whole-animal manipulation for high-throughput subcellular-resolution in-vivo drug/genetic screening.” Life Science Systems and Applications Workshop, 2009. LiSSA 2009. IEEE/NIH. 2009. 52-55. © 2009 IEEE. https://orcid.org/0000-0002-4612-1962 en_US http://dx.doi.org/10.1109/LISSA.2009.4906707 IEEE/NIH Life Science Systems and Applications Workshop, 2009. LiSSA 2009 Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Institute of Electrical and Electronics Engineers IEEE |
| spellingShingle | Yanik, Mehmet Fatih Rohde, Christopher Benjamin Gilleland, Cody Lee Samara, Chrysanthi On-chip whole-animal manipulation for high-throughput subcellular-resolution in-vivo drug/genetic screening |
| title | On-chip whole-animal manipulation for high-throughput subcellular-resolution in-vivo drug/genetic screening |
| title_full | On-chip whole-animal manipulation for high-throughput subcellular-resolution in-vivo drug/genetic screening |
| title_fullStr | On-chip whole-animal manipulation for high-throughput subcellular-resolution in-vivo drug/genetic screening |
| title_full_unstemmed | On-chip whole-animal manipulation for high-throughput subcellular-resolution in-vivo drug/genetic screening |
| title_short | On-chip whole-animal manipulation for high-throughput subcellular-resolution in-vivo drug/genetic screening |
| title_sort | on chip whole animal manipulation for high throughput subcellular resolution in vivo drug genetic screening |
| url | http://hdl.handle.net/1721.1/60276 https://orcid.org/0000-0002-4612-1962 |
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