Receptor Specificity and Transmission of H2N2 Subtype Viruses Isolated from the Pandemic of 1957
Influenza viruses of the H2N2 subtype have not circulated among humans in over 40 years. The occasional isolation of avian H2 strains from swine and avian species coupled with waning population immunity to H2 hemagglutinin (HA) warrants investigation of this subtype due to its pandemic potential. In...
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| Format: | Article |
| Language: | en_US |
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Public Library of Science
2010
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| Online Access: | http://hdl.handle.net/1721.1/60368 https://orcid.org/0000-0002-1288-9965 https://orcid.org/0000-0002-2085-7840 |
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| author | Pappas, Claudia Viswanathan, Karthik Chandrasekaran, Aarthi Raman, Rahul Katz, Jacqueline M. Sasisekharan, Ram Tumpey, Terrence M. |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Pappas, Claudia Viswanathan, Karthik Chandrasekaran, Aarthi Raman, Rahul Katz, Jacqueline M. Sasisekharan, Ram Tumpey, Terrence M. |
| author_sort | Pappas, Claudia |
| collection | MIT |
| description | Influenza viruses of the H2N2 subtype have not circulated among humans in over 40 years. The occasional isolation of avian H2 strains from swine and avian species coupled with waning population immunity to H2 hemagglutinin (HA) warrants investigation of this subtype due to its pandemic potential. In this study we examined the transmissibility of representative human H2N2 viruses, A/Albany/6/58 (Alb/58) and A/El Salvador/2/57 (ElSalv/57), isolated during the 1957/58 pandemic, in the ferret model. The receptor binding properties of these H2N2 viruses was analyzed using dose-dependent direct glycan array-binding assays. Alb/58 virus, which contains the 226L/228S amino acid combination in the HA and displayed dual binding to both alpha 2,6 and alpha 2,3 glycan receptors, transmitted efficiently to naïve ferrets by respiratory droplets. Inefficient transmission was observed with ElSalv/57 virus, which contains the 226Q/228G amino acid combination and preferentially binds alpha 2,3 over alpha 2,6 glycan receptors. However, a unique transmission event with the ElSalv/57 virus occurred which produced a 226L/228G H2N2 natural variant virus that displayed an increase in binding specificity to alpha 2,6 glycan receptors and enhanced respiratory droplet transmissibility. Our studies provide a correlation between binding affinity to glycan receptors with terminal alpha 2,6-linked sialic acid and the efficiency of respiratory droplet transmission for pandemic H2N2 influenza viruses. |
| first_indexed | 2024-09-23T15:00:39Z |
| format | Article |
| id | mit-1721.1/60368 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T15:00:39Z |
| publishDate | 2010 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | mit-1721.1/603682022-09-29T12:02:38Z Receptor Specificity and Transmission of H2N2 Subtype Viruses Isolated from the Pandemic of 1957 Pappas, Claudia Viswanathan, Karthik Chandrasekaran, Aarthi Raman, Rahul Katz, Jacqueline M. Sasisekharan, Ram Tumpey, Terrence M. Harvard University--MIT Division of Health Sciences and Technology Massachusetts Institute of Technology. Department of Biological Engineering Koch Institute for Integrative Cancer Research at MIT Sasisekharan, Ram Viswanathan, Karthik Chandrasekaran, Aarthi Raman, Rahul Sasisekharan, Ram Influenza viruses of the H2N2 subtype have not circulated among humans in over 40 years. The occasional isolation of avian H2 strains from swine and avian species coupled with waning population immunity to H2 hemagglutinin (HA) warrants investigation of this subtype due to its pandemic potential. In this study we examined the transmissibility of representative human H2N2 viruses, A/Albany/6/58 (Alb/58) and A/El Salvador/2/57 (ElSalv/57), isolated during the 1957/58 pandemic, in the ferret model. The receptor binding properties of these H2N2 viruses was analyzed using dose-dependent direct glycan array-binding assays. Alb/58 virus, which contains the 226L/228S amino acid combination in the HA and displayed dual binding to both alpha 2,6 and alpha 2,3 glycan receptors, transmitted efficiently to naïve ferrets by respiratory droplets. Inefficient transmission was observed with ElSalv/57 virus, which contains the 226Q/228G amino acid combination and preferentially binds alpha 2,3 over alpha 2,6 glycan receptors. However, a unique transmission event with the ElSalv/57 virus occurred which produced a 226L/228G H2N2 natural variant virus that displayed an increase in binding specificity to alpha 2,6 glycan receptors and enhanced respiratory droplet transmissibility. Our studies provide a correlation between binding affinity to glycan receptors with terminal alpha 2,6-linked sialic acid and the efficiency of respiratory droplet transmission for pandemic H2N2 influenza viruses. Centers for Disease Control and Prevention (U.S.) National Institutes of Health (U.S.) (grant U54 GM62116) Singapore-MIT Alliance for Research and Technology 2010-12-22T20:02:47Z 2010-12-22T20:02:47Z 2010-06 2010-01 Article http://purl.org/eprint/type/JournalArticle 1932-6203 http://hdl.handle.net/1721.1/60368 Pappas, Claudia et al. “Receptor Specificity and Transmission of H2N2 Subtype Viruses Isolated from the Pandemic of 1957.” PLoS ONE 5.6 (2010): e11158. https://orcid.org/0000-0002-1288-9965 https://orcid.org/0000-0002-2085-7840 en_US http://dx.doi.org/10.1371/journal.pone.0011158 PLoS ONE Creative Commons Attribution http://creativecommons.org/licenses/by/2.5/ application/pdf Public Library of Science PLoS |
| spellingShingle | Pappas, Claudia Viswanathan, Karthik Chandrasekaran, Aarthi Raman, Rahul Katz, Jacqueline M. Sasisekharan, Ram Tumpey, Terrence M. Receptor Specificity and Transmission of H2N2 Subtype Viruses Isolated from the Pandemic of 1957 |
| title | Receptor Specificity and Transmission of H2N2 Subtype Viruses Isolated from the Pandemic of 1957 |
| title_full | Receptor Specificity and Transmission of H2N2 Subtype Viruses Isolated from the Pandemic of 1957 |
| title_fullStr | Receptor Specificity and Transmission of H2N2 Subtype Viruses Isolated from the Pandemic of 1957 |
| title_full_unstemmed | Receptor Specificity and Transmission of H2N2 Subtype Viruses Isolated from the Pandemic of 1957 |
| title_short | Receptor Specificity and Transmission of H2N2 Subtype Viruses Isolated from the Pandemic of 1957 |
| title_sort | receptor specificity and transmission of h2n2 subtype viruses isolated from the pandemic of 1957 |
| url | http://hdl.handle.net/1721.1/60368 https://orcid.org/0000-0002-1288-9965 https://orcid.org/0000-0002-2085-7840 |
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