O-antigen diversity and lateral transfer of the wbe region among Vibrio splendidus isolates
The O-antigen is a highly diverse structure expressed on the outer surface of Gram-negative bacteria. The products responsible for O-antigen synthesis are encoded in the wbe region, which exhibits extensive genetic diversity. While heterogeneous O-antigens are observed within Vibrio species, charact...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | en_US |
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Blackwell Publishing
2011
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| Online Access: | http://hdl.handle.net/1721.1/60921 https://orcid.org/0000-0001-9296-3733 |
| _version_ | 1811073366029762560 |
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| author | Wildschutte, Hans Preheim, Sarah Pacocha Hernandez, Yasiel Polz, Martin F. |
| author2 | Massachusetts Institute of Technology. Department of Civil and Environmental Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Civil and Environmental Engineering Wildschutte, Hans Preheim, Sarah Pacocha Hernandez, Yasiel Polz, Martin F. |
| author_sort | Wildschutte, Hans |
| collection | MIT |
| description | The O-antigen is a highly diverse structure expressed on the outer surface of Gram-negative bacteria. The products responsible for O-antigen synthesis are encoded in the wbe region, which exhibits extensive genetic diversity. While heterogeneous O-antigens are observed within Vibrio species, characterization of these structures has been devoted almost exclusively to pathogens. Here, we investigate O-antigen diversity among coastal marine Vibrio splendidus-like isolates. The wbe region was first identified and characterized using the sequenced genomes of strains LGP32, 12B01 and Med222. These regions were genetically diverse, reflective of their expressed O-antigen. Additional isolates from physically distinct habitats in Plum Island Estuary (MA, USA), including within animal hosts and on suspended particles, were further characterized based on multilocus sequence analysis (MLSA) and O-antigen profiles. Results showed serotype diversity within an ecological setting. Among 48 isolates which were identical in three MLSA genes, 41 showed gpm genetic diversity, a gene closely linked to the wbe locus, and at least 12 expressed different O-antigen profiles further suggesting wbe genetic diversity. Our results demonstrate O-antigen hyper-variability among these environmental strains and suggest that frequent lateral gene transfer generates wbe extensive diversity among V. splendidus and its close relatives. |
| first_indexed | 2024-09-23T09:31:56Z |
| format | Article |
| id | mit-1721.1/60921 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T09:31:56Z |
| publishDate | 2011 |
| publisher | Blackwell Publishing |
| record_format | dspace |
| spelling | mit-1721.1/609212022-09-26T12:05:30Z O-antigen diversity and lateral transfer of the wbe region among Vibrio splendidus isolates Wildschutte, Hans Preheim, Sarah Pacocha Hernandez, Yasiel Polz, Martin F. Massachusetts Institute of Technology. Department of Civil and Environmental Engineering Wildschutte, Hans Hernandez, Yasiel Wildschutte, Hans Preheim, Sarah Pacocha Polz, Martin F. The O-antigen is a highly diverse structure expressed on the outer surface of Gram-negative bacteria. The products responsible for O-antigen synthesis are encoded in the wbe region, which exhibits extensive genetic diversity. While heterogeneous O-antigens are observed within Vibrio species, characterization of these structures has been devoted almost exclusively to pathogens. Here, we investigate O-antigen diversity among coastal marine Vibrio splendidus-like isolates. The wbe region was first identified and characterized using the sequenced genomes of strains LGP32, 12B01 and Med222. These regions were genetically diverse, reflective of their expressed O-antigen. Additional isolates from physically distinct habitats in Plum Island Estuary (MA, USA), including within animal hosts and on suspended particles, were further characterized based on multilocus sequence analysis (MLSA) and O-antigen profiles. Results showed serotype diversity within an ecological setting. Among 48 isolates which were identical in three MLSA genes, 41 showed gpm genetic diversity, a gene closely linked to the wbe locus, and at least 12 expressed different O-antigen profiles further suggesting wbe genetic diversity. Our results demonstrate O-antigen hyper-variability among these environmental strains and suggest that frequent lateral gene transfer generates wbe extensive diversity among V. splendidus and its close relatives. National Institute of General Medical Sciences (U.S.) (F32GM084640) United States. Dept. of Energy (Grant no. DE-FG09-93ER-20097) Woods Hole Center for Oceans and Human Health Gordon and Betty Moore Foundation 2011-02-11T16:17:56Z 2011-02-11T16:17:56Z 2010-11 2010-03 Article http://purl.org/eprint/type/JournalArticle 1462-2920 http://hdl.handle.net/1721.1/60921 Wildschutte, H., Preheim, S. P., Hernandez, Y. and Polz, M. F. (2010), O-antigen diversity and lateral transfer of the wbe region among Vibrio splendidus isolates. Environmental Microbiology, 12: 2977–2987. doi: 10.1111/j.1462-2920.2010.02274.x https://orcid.org/0000-0001-9296-3733 en_US http://dx.doi.org/10.1111/j.1462-2920.2010.02274.x Environmental Microbiology Attribution-Noncommercial-Share Alike 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf Blackwell Publishing Prof. Wildschutte via Anne Graham |
| spellingShingle | Wildschutte, Hans Preheim, Sarah Pacocha Hernandez, Yasiel Polz, Martin F. O-antigen diversity and lateral transfer of the wbe region among Vibrio splendidus isolates |
| title | O-antigen diversity and lateral transfer of the wbe region among Vibrio splendidus isolates |
| title_full | O-antigen diversity and lateral transfer of the wbe region among Vibrio splendidus isolates |
| title_fullStr | O-antigen diversity and lateral transfer of the wbe region among Vibrio splendidus isolates |
| title_full_unstemmed | O-antigen diversity and lateral transfer of the wbe region among Vibrio splendidus isolates |
| title_short | O-antigen diversity and lateral transfer of the wbe region among Vibrio splendidus isolates |
| title_sort | o antigen diversity and lateral transfer of the wbe region among vibrio splendidus isolates |
| url | http://hdl.handle.net/1721.1/60921 https://orcid.org/0000-0001-9296-3733 |
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