Shack-Hartmann wavefront-sensor-based adaptive optics system for microscopy
The imaging depth of two-photon excitation fluorescence microscopy is partly limited by the inhomogeneity of the refractive index in biological specimens. This inhomogeneity results in a distortion of the wavefront of the excitation light. This wavefront distortion results in image resolution degrad...
| Main Authors: | , , |
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| Drugi avtorji: | |
| Format: | Article |
| Jezik: | en_US |
| Izdano: |
SPIE (Society)
2011
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| Online dostop: | http://hdl.handle.net/1721.1/61364 https://orcid.org/0000-0003-4698-6488 |
| Izvleček: | The imaging depth of two-photon excitation fluorescence microscopy is partly limited by the inhomogeneity of the refractive index in biological specimens. This inhomogeneity results in a distortion of the wavefront of the excitation light. This wavefront distortion results in image resolution degradation and lower signal level. Using an adaptive optics system consisting of a Shack-Hartmann wavefront sensor and a deformable mirror, wavefront distortion can be measured and corrected. With adaptive optics compensation, we demonstrate that the resolution and signal level can be better preserved at greater imaging depth in a variety of ex-vivo tissue specimens including mouse tongue muscle, heart muscle, and brain. However, for these highly scattering tissues, we find signal degradation due to scattering to be a more dominant factor than aberration. |
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