Shack-Hartmann wavefront-sensor-based adaptive optics system for microscopy
The imaging depth of two-photon excitation fluorescence microscopy is partly limited by the inhomogeneity of the refractive index in biological specimens. This inhomogeneity results in a distortion of the wavefront of the excitation light. This wavefront distortion results in image resolution degrad...
| Main Authors: | , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
SPIE (Society)
2011
|
| Online Access: | http://hdl.handle.net/1721.1/61364 https://orcid.org/0000-0003-4698-6488 |
| _version_ | 1826215965624369152 |
|---|---|
| author | So, Peter T. C. Cha, Jae Won Ballesta, Jerome |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering So, Peter T. C. Cha, Jae Won Ballesta, Jerome |
| author_sort | So, Peter T. C. |
| collection | MIT |
| description | The imaging depth of two-photon excitation fluorescence microscopy is partly limited by the inhomogeneity of the refractive index in biological specimens. This inhomogeneity results in a distortion of the wavefront of the excitation light. This wavefront distortion results in image resolution degradation and lower signal level. Using an adaptive optics system consisting of a Shack-Hartmann wavefront sensor and a deformable mirror, wavefront distortion can be measured and corrected. With adaptive optics compensation, we demonstrate that the resolution and signal level can be better preserved at greater imaging depth in a variety of ex-vivo tissue specimens including mouse tongue muscle, heart muscle, and brain. However, for these highly scattering tissues, we find signal degradation due to scattering to be a more dominant factor than aberration. |
| first_indexed | 2024-09-23T16:40:15Z |
| format | Article |
| id | mit-1721.1/61364 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T16:40:15Z |
| publishDate | 2011 |
| publisher | SPIE (Society) |
| record_format | dspace |
| spelling | mit-1721.1/613642022-09-29T20:39:17Z Shack-Hartmann wavefront-sensor-based adaptive optics system for microscopy So, Peter T. C. Cha, Jae Won Ballesta, Jerome Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Mechanical Engineering So, Peter T. C. So, Peter T. C. Cha, Jae Won The imaging depth of two-photon excitation fluorescence microscopy is partly limited by the inhomogeneity of the refractive index in biological specimens. This inhomogeneity results in a distortion of the wavefront of the excitation light. This wavefront distortion results in image resolution degradation and lower signal level. Using an adaptive optics system consisting of a Shack-Hartmann wavefront sensor and a deformable mirror, wavefront distortion can be measured and corrected. With adaptive optics compensation, we demonstrate that the resolution and signal level can be better preserved at greater imaging depth in a variety of ex-vivo tissue specimens including mouse tongue muscle, heart muscle, and brain. However, for these highly scattering tissues, we find signal degradation due to scattering to be a more dominant factor than aberration. Singapore-MIT Alliance Singapore-MIT Alliance for Research and Technology Center National Institutes of Health (U.S.) (Grant RO1 EY017656) 2011-03-01T22:46:00Z 2011-03-01T22:46:00Z 2010-08 2010-02 Article http://purl.org/eprint/type/JournalArticle 1083-3668 http://hdl.handle.net/1721.1/61364 Cha, Jae Won, Jerome Ballesta, and Peter T. C. So. “Shack-Hartmann wavefront-sensor-based adaptive optics system for multiphoton microscopy.” Journal of Biomedical Optics 15.4 (2010): 046022. © 2010 Society of Photo-Optical Instrumentation Engineers SPIE https://orcid.org/0000-0003-4698-6488 en_US http://dx.doi.org/10.1117/1.3475954 Journal of Biomedical Optics Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf SPIE (Society) SPIE |
| spellingShingle | So, Peter T. C. Cha, Jae Won Ballesta, Jerome Shack-Hartmann wavefront-sensor-based adaptive optics system for microscopy |
| title | Shack-Hartmann wavefront-sensor-based adaptive optics system for microscopy |
| title_full | Shack-Hartmann wavefront-sensor-based adaptive optics system for microscopy |
| title_fullStr | Shack-Hartmann wavefront-sensor-based adaptive optics system for microscopy |
| title_full_unstemmed | Shack-Hartmann wavefront-sensor-based adaptive optics system for microscopy |
| title_short | Shack-Hartmann wavefront-sensor-based adaptive optics system for microscopy |
| title_sort | shack hartmann wavefront sensor based adaptive optics system for microscopy |
| url | http://hdl.handle.net/1721.1/61364 https://orcid.org/0000-0003-4698-6488 |
| work_keys_str_mv | AT sopetertc shackhartmannwavefrontsensorbasedadaptiveopticssystemformicroscopy AT chajaewon shackhartmannwavefrontsensorbasedadaptiveopticssystemformicroscopy AT ballestajerome shackhartmannwavefrontsensorbasedadaptiveopticssystemformicroscopy |