Nanofabricated collagen-inspired synthetic elastomers for primary rat hepatocyte culture
Synthetic substrates that mimic the properties of extracellular matrix proteins hold significant promise for use in systems designed for tissue engineering applications. In this report, we designed a synthetic polymeric substrate that is intended to mimic chemical, mechanical, and topological charac...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
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Mary Ann Liebert, Inc.
2011
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| Online Access: | http://hdl.handle.net/1721.1/61681 https://orcid.org/0000-0003-4255-0492 |
| _version_ | 1811081528024760320 |
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| author | Bettinger, Christopher J. Kulig, Katherine M. Vacanti, Joseph P. Langer, Robert Borenstein, Jeffrey T. |
| author2 | Charles Stark Draper Laboratory |
| author_facet | Charles Stark Draper Laboratory Bettinger, Christopher J. Kulig, Katherine M. Vacanti, Joseph P. Langer, Robert Borenstein, Jeffrey T. |
| author_sort | Bettinger, Christopher J. |
| collection | MIT |
| description | Synthetic substrates that mimic the properties of extracellular matrix proteins hold significant promise for use in systems designed for tissue engineering applications. In this report, we designed a synthetic polymeric substrate that is intended to mimic chemical, mechanical, and topological characteristics of collagen. We found that elastomeric poly(ester amide) substrates modified with replica-molded nanotopographic features enhanced initial attachment, spreading, and adhesion of primary rat hepatocytes. Further, hepatocytes cultured on nanotopographic substrates also demonstrated reduced albumin secretion and urea synthesis, which is indicative of strongly adherent hepatocytes. These results suggest that these engineered substrates can function as synthetic collagen analogs for in vitro cell culture. |
| first_indexed | 2024-09-23T11:47:58Z |
| format | Article |
| id | mit-1721.1/61681 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T11:47:58Z |
| publishDate | 2011 |
| publisher | Mary Ann Liebert, Inc. |
| record_format | dspace |
| spelling | mit-1721.1/616812022-09-27T21:58:12Z Nanofabricated collagen-inspired synthetic elastomers for primary rat hepatocyte culture Bettinger, Christopher J. Kulig, Katherine M. Vacanti, Joseph P. Langer, Robert Borenstein, Jeffrey T. Charles Stark Draper Laboratory Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Chemical Engineering Massachusetts Institute of Technology. Department of Materials Science and Engineering Langer, Robert Bettinger, Christopher J. Langer, Robert Borenstein, Jeffrey T. Synthetic substrates that mimic the properties of extracellular matrix proteins hold significant promise for use in systems designed for tissue engineering applications. In this report, we designed a synthetic polymeric substrate that is intended to mimic chemical, mechanical, and topological characteristics of collagen. We found that elastomeric poly(ester amide) substrates modified with replica-molded nanotopographic features enhanced initial attachment, spreading, and adhesion of primary rat hepatocytes. Further, hepatocytes cultured on nanotopographic substrates also demonstrated reduced albumin secretion and urea synthesis, which is indicative of strongly adherent hepatocytes. These results suggest that these engineered substrates can function as synthetic collagen analogs for in vitro cell culture. 2011-03-11T20:26:07Z 2011-03-11T20:26:07Z 2009-07 2008-02 Article http://purl.org/eprint/type/JournalArticle 1937-3341 1937-335X http://hdl.handle.net/1721.1/61681 Bettinger, Christopher J. et al. “Nanofabricated Collagen-Inspired Synthetic Elastomers for Primary Rat Hepatocyte Culture.” Tissue Engineering Part A 15.6 (2009): 1321-1329. ©2009 Mary Ann Liebert, Inc. https://orcid.org/0000-0003-4255-0492 en_US http://dx.doi.org/10.1089/ten.tea.2008.0134 Tissue Engineering. Part A Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Mary Ann Liebert, Inc. Mary Ann Liebert |
| spellingShingle | Bettinger, Christopher J. Kulig, Katherine M. Vacanti, Joseph P. Langer, Robert Borenstein, Jeffrey T. Nanofabricated collagen-inspired synthetic elastomers for primary rat hepatocyte culture |
| title | Nanofabricated collagen-inspired synthetic elastomers for primary rat hepatocyte culture |
| title_full | Nanofabricated collagen-inspired synthetic elastomers for primary rat hepatocyte culture |
| title_fullStr | Nanofabricated collagen-inspired synthetic elastomers for primary rat hepatocyte culture |
| title_full_unstemmed | Nanofabricated collagen-inspired synthetic elastomers for primary rat hepatocyte culture |
| title_short | Nanofabricated collagen-inspired synthetic elastomers for primary rat hepatocyte culture |
| title_sort | nanofabricated collagen inspired synthetic elastomers for primary rat hepatocyte culture |
| url | http://hdl.handle.net/1721.1/61681 https://orcid.org/0000-0003-4255-0492 |
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