Evidence for In Vivo Growth Potential and Vascular Remodeling of Tissue-Engineered Artery
Nondegradable synthetic polymer vascular grafts currently used in cardiovascular surgery have no growth potential. Tissue-engineered vascular grafts (TEVGs) may solve this problem. In this study, we developed a TEVG using autologous bone marrow–derived cells (BMCs) and decellularized tissue matric...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Mary Ann Liebert
2011
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| Online Access: | http://hdl.handle.net/1721.1/61684 |
| _version_ | 1826205025596080128 |
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| author | Cho, Seung Woo Kim, Il-Kwon Kang, Jin Muk Song, Kang Won Kim, Hong Sik Park, Chang Hwan Yoo, Kyung Jong Kim, Byung-Soo |
| author2 | Massachusetts Institute of Technology. Department of Chemical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Chemical Engineering Cho, Seung Woo Kim, Il-Kwon Kang, Jin Muk Song, Kang Won Kim, Hong Sik Park, Chang Hwan Yoo, Kyung Jong Kim, Byung-Soo |
| author_sort | Cho, Seung Woo |
| collection | MIT |
| description | Nondegradable synthetic polymer vascular grafts currently used in cardiovascular surgery have no growth
potential. Tissue-engineered vascular grafts (TEVGs) may solve this problem. In this study, we developed a TEVG
using autologous bone marrow–derived cells (BMCs) and decellularized tissue matrices, and tested whether
the TEVGs exhibit growth potential and vascular remodeling in vivo. Vascular smooth muscle–like cells and
endothelial-like cells were differentiated from bone marrow mononuclear cells in vitro. TEVGs were fabricated by
seeding these cells onto decellularized porcine abdominal aortas and implanted into the abdominal aortas of
4-month-old, bone marrow donor pigs (n¼4). Eighteen weeks after implantation, the dimensions of TEVGs were
measured and compared with those of native abdominal aortas. Expression of molecules associated with vascular
remodeling was examined with reverse transcription–polymerase chain reaction assay and immunohistochemistry.
Eighteen weeks after implantation, all TEVGs were patent with no sign of thrombus formation, dilatation, or
stenosis. Histological and immunohistochemical analyses of the retrieved TEVGs revealed regeneration of endothelium
and smooth muscle and the presence of collagen and elastin. The outer diameter of three of the four
TEVGs increased in proportion to increases in body weight and outer native aorta diameter. Considerable extents
of expression of molecules associated with extracellular matrix (ECM) degradation (i.e., matrix metalloproteinase
and tissue inhibitor of matrix metalloproteinase) and ECM precursors (i.e., procollagen I, procollagen III, and
tropoelastin) occurred in the TEVGs, indicating vascular remodeling associated with degradation of exogenous
ECMs (implanted decellularized matrices) and synthesis of autologous ECMs. This study demonstrates that the
TEVGs with autologous BMCs and decellularized tissue matrices exhibit growth potential and vascular remodeling
in vivo of tissue-engineered artery. |
| first_indexed | 2024-09-23T13:05:34Z |
| format | Article |
| id | mit-1721.1/61684 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T13:05:34Z |
| publishDate | 2011 |
| publisher | Mary Ann Liebert |
| record_format | dspace |
| spelling | mit-1721.1/616842022-09-28T11:58:06Z Evidence for In Vivo Growth Potential and Vascular Remodeling of Tissue-Engineered Artery Cho, Seung Woo Kim, Il-Kwon Kang, Jin Muk Song, Kang Won Kim, Hong Sik Park, Chang Hwan Yoo, Kyung Jong Kim, Byung-Soo Massachusetts Institute of Technology. Department of Chemical Engineering Cho, Seung Woo Cho, Seung Woo Nondegradable synthetic polymer vascular grafts currently used in cardiovascular surgery have no growth potential. Tissue-engineered vascular grafts (TEVGs) may solve this problem. In this study, we developed a TEVG using autologous bone marrow–derived cells (BMCs) and decellularized tissue matrices, and tested whether the TEVGs exhibit growth potential and vascular remodeling in vivo. Vascular smooth muscle–like cells and endothelial-like cells were differentiated from bone marrow mononuclear cells in vitro. TEVGs were fabricated by seeding these cells onto decellularized porcine abdominal aortas and implanted into the abdominal aortas of 4-month-old, bone marrow donor pigs (n¼4). Eighteen weeks after implantation, the dimensions of TEVGs were measured and compared with those of native abdominal aortas. Expression of molecules associated with vascular remodeling was examined with reverse transcription–polymerase chain reaction assay and immunohistochemistry. Eighteen weeks after implantation, all TEVGs were patent with no sign of thrombus formation, dilatation, or stenosis. Histological and immunohistochemical analyses of the retrieved TEVGs revealed regeneration of endothelium and smooth muscle and the presence of collagen and elastin. The outer diameter of three of the four TEVGs increased in proportion to increases in body weight and outer native aorta diameter. Considerable extents of expression of molecules associated with extracellular matrix (ECM) degradation (i.e., matrix metalloproteinase and tissue inhibitor of matrix metalloproteinase) and ECM precursors (i.e., procollagen I, procollagen III, and tropoelastin) occurred in the TEVGs, indicating vascular remodeling associated with degradation of exogenous ECMs (implanted decellularized matrices) and synthesis of autologous ECMs. This study demonstrates that the TEVGs with autologous BMCs and decellularized tissue matrices exhibit growth potential and vascular remodeling in vivo of tissue-engineered artery. Korea. Ministry of Health and Welfare (Grant A050082) (Grant 02-PJ10- PG8-EC01-0016) 2011-03-11T21:05:48Z 2011-03-11T21:05:48Z 2008-09 Article http://purl.org/eprint/type/JournalArticle 1937-3341 1937-335X http://hdl.handle.net/1721.1/61684 Cho, Seung-Woo et al. "Evidence for <i>In Vivo</i> Growth Potential and Vascular Remodeling of Tissue-Engineered Artery." Tissue Engineering Part A 15.4 (2009): 901-912. © 2009, Mary Ann Liebert, Inc. en_US http://dx.doi.org/10.1089/ten.tea.2008.0172 Tissue Engineering. Part A Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Mary Ann Liebert Mary Ann Liebert |
| spellingShingle | Cho, Seung Woo Kim, Il-Kwon Kang, Jin Muk Song, Kang Won Kim, Hong Sik Park, Chang Hwan Yoo, Kyung Jong Kim, Byung-Soo Evidence for In Vivo Growth Potential and Vascular Remodeling of Tissue-Engineered Artery |
| title | Evidence for In Vivo Growth Potential and Vascular Remodeling of Tissue-Engineered Artery |
| title_full | Evidence for In Vivo Growth Potential and Vascular Remodeling of Tissue-Engineered Artery |
| title_fullStr | Evidence for In Vivo Growth Potential and Vascular Remodeling of Tissue-Engineered Artery |
| title_full_unstemmed | Evidence for In Vivo Growth Potential and Vascular Remodeling of Tissue-Engineered Artery |
| title_short | Evidence for In Vivo Growth Potential and Vascular Remodeling of Tissue-Engineered Artery |
| title_sort | evidence for in vivo growth potential and vascular remodeling of tissue engineered artery |
| url | http://hdl.handle.net/1721.1/61684 |
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