Three-Dimensional Hydrogel Model Using Adipose-Derived Stem Cells for Vocal Fold Augmentation
Adipose-derived stem cells (ASCs) may provide a clinical option for rebuilding damaged superficial lamina propria of the vocal fold. We investigated the effects of five hydrogels (hyaluronic acid [HA], collagen, fibrin, and cogels of fibrin–collagen and fibrin–HA) on the differentiation of ASCs, wit...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Mary Ann Liebert, Inc.
2011
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| Online Access: | http://hdl.handle.net/1721.1/62175 https://orcid.org/0000-0002-9765-446X https://orcid.org/0000-0003-4255-0492 |
| _version_ | 1826199700828585984 |
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| author | Park, Hyoungshin Karajanagi, Sandeep S. Wolak, Kathryn Aanestad, Jon Daheron, Laurence Kobler, James B. Lopez-Guerra, Gerardo Heaton, James T. Zeitels, Steven M. Langer, Robert S |
| author2 | Massachusetts Institute of Technology. Department of Chemical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Chemical Engineering Park, Hyoungshin Karajanagi, Sandeep S. Wolak, Kathryn Aanestad, Jon Daheron, Laurence Kobler, James B. Lopez-Guerra, Gerardo Heaton, James T. Zeitels, Steven M. Langer, Robert S |
| author_sort | Park, Hyoungshin |
| collection | MIT |
| description | Adipose-derived stem cells (ASCs) may provide a clinical option for rebuilding damaged superficial lamina propria of the vocal fold. We investigated the effects of five hydrogels (hyaluronic acid [HA], collagen, fibrin, and cogels of fibrin–collagen and fibrin–HA) on the differentiation of ASCs, with the long-term goal of establishing the conditions necessary for controlling the differentiation of ASC into the functional equivalent of superficial lamina propria fibroblasts. Human ASCs were isolated and characterized by fluorescence-activated cell sorting and real-time polymerase chain reaction. According to fluorescence-activated cell sorting and gene analysis, over 90% of isolated ASCs expressed adult stem cell surface markers and expressed adult stem cell genes. Scaffold-specific gene expression and morphology were assessed by culturing the ASCs in three-dimensional hydrogels. Twofold higher amounts of total DNA were detected in fibrin and cogel cultures than in collagen and HA cultures. Elastin expression was significantly higher in cells grown in fibrin-based gels than in cells grown in other gels. Cells grown in the cogels showed elongated morphology, expressed decorin marker, and exhibited glycosaminoglycan synthesis, which indicate ASC differentiation. Our data suggest that it may be possible to control the differentiation of ASCs using scaffolds appropriate for vocal fold tissue engineering applications. In particular, cogels of HA or collagen with fibrin enhanced proliferation, differentiation, and elastin expression. |
| first_indexed | 2024-09-23T11:24:12Z |
| format | Article |
| id | mit-1721.1/62175 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T11:24:12Z |
| publishDate | 2011 |
| publisher | Mary Ann Liebert, Inc. |
| record_format | dspace |
| spelling | mit-1721.1/621752022-10-01T03:23:00Z Three-Dimensional Hydrogel Model Using Adipose-Derived Stem Cells for Vocal Fold Augmentation Park, Hyoungshin Karajanagi, Sandeep S. Wolak, Kathryn Aanestad, Jon Daheron, Laurence Kobler, James B. Lopez-Guerra, Gerardo Heaton, James T. Zeitels, Steven M. Langer, Robert S Massachusetts Institute of Technology. Department of Chemical Engineering Koch Institute for Integrative Cancer Research at MIT Langer, Robert Langer, Robert Karajanagi, Sandeep S. Adipose-derived stem cells (ASCs) may provide a clinical option for rebuilding damaged superficial lamina propria of the vocal fold. We investigated the effects of five hydrogels (hyaluronic acid [HA], collagen, fibrin, and cogels of fibrin–collagen and fibrin–HA) on the differentiation of ASCs, with the long-term goal of establishing the conditions necessary for controlling the differentiation of ASC into the functional equivalent of superficial lamina propria fibroblasts. Human ASCs were isolated and characterized by fluorescence-activated cell sorting and real-time polymerase chain reaction. According to fluorescence-activated cell sorting and gene analysis, over 90% of isolated ASCs expressed adult stem cell surface markers and expressed adult stem cell genes. Scaffold-specific gene expression and morphology were assessed by culturing the ASCs in three-dimensional hydrogels. Twofold higher amounts of total DNA were detected in fibrin and cogel cultures than in collagen and HA cultures. Elastin expression was significantly higher in cells grown in fibrin-based gels than in cells grown in other gels. Cells grown in the cogels showed elongated morphology, expressed decorin marker, and exhibited glycosaminoglycan synthesis, which indicate ASC differentiation. Our data suggest that it may be possible to control the differentiation of ASCs using scaffolds appropriate for vocal fold tissue engineering applications. In particular, cogels of HA or collagen with fibrin enhanced proliferation, differentiation, and elastin expression. Eugene B. Casey Foundation Institute of Laryngology and Voice Restoration 2011-04-08T16:45:11Z 2011-04-08T16:45:11Z 2010-02 Article http://purl.org/eprint/type/JournalArticle 1937-3341 1937-335X http://hdl.handle.net/1721.1/62175 Park, Hyoungshin et al. “Three-Dimensional Hydrogel Model Using Adipose-Derived Stem Cells for Vocal Fold Augmentation.” Tissue Engineering Part A 16.2 (2010) : 535-543. © 2010 Mary Ann Liebert, Inc., publishers . https://orcid.org/0000-0002-9765-446X https://orcid.org/0000-0003-4255-0492 en_US http://dx.doi.org/10.1089/ten.tea.2009.0029 Tissue Engineering, Part A Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Mary Ann Liebert, Inc. Mary Ann Liebert |
| spellingShingle | Park, Hyoungshin Karajanagi, Sandeep S. Wolak, Kathryn Aanestad, Jon Daheron, Laurence Kobler, James B. Lopez-Guerra, Gerardo Heaton, James T. Zeitels, Steven M. Langer, Robert S Three-Dimensional Hydrogel Model Using Adipose-Derived Stem Cells for Vocal Fold Augmentation |
| title | Three-Dimensional Hydrogel Model Using Adipose-Derived Stem Cells for Vocal Fold Augmentation |
| title_full | Three-Dimensional Hydrogel Model Using Adipose-Derived Stem Cells for Vocal Fold Augmentation |
| title_fullStr | Three-Dimensional Hydrogel Model Using Adipose-Derived Stem Cells for Vocal Fold Augmentation |
| title_full_unstemmed | Three-Dimensional Hydrogel Model Using Adipose-Derived Stem Cells for Vocal Fold Augmentation |
| title_short | Three-Dimensional Hydrogel Model Using Adipose-Derived Stem Cells for Vocal Fold Augmentation |
| title_sort | three dimensional hydrogel model using adipose derived stem cells for vocal fold augmentation |
| url | http://hdl.handle.net/1721.1/62175 https://orcid.org/0000-0002-9765-446X https://orcid.org/0000-0003-4255-0492 |
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