Direct Detection of Nitroxyl in Aqueous Solution using a Tripodal Copper(II) BODIPY Complex
Nitric oxide (NO) mediates both physiological and pathological processes.1,2 In addition to cardiovascular signaling, NO has been invoked to play a neurochemical role in learning and memory, and it is a powerful necrotic agent wielded by macrophages of the immune system. Whereas considerable eff...
| Main Authors: | , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
American Chemical Society
2011
|
| Online Access: | http://hdl.handle.net/1721.1/64771 https://orcid.org/0000-0002-2693-4982 |
| Summary: | Nitric oxide (NO) mediates both physiological and pathological
processes.1,2 In addition to cardiovascular signaling, NO has been
invoked to play a neurochemical role in learning and memory, and
it is a powerful necrotic agent wielded by macrophages of the
immune system. Whereas considerable effort has been invested to
develop metal-based3-5 and other6,7 probes for detecting nitric
oxide, there has been significantly less progress in the synthesis of
platforms capable of detecting other reactive nitrogen species
(RNS).8 Of the nitrogen oxides relevant to biology, nitroxyl
(HNO), the one electron reduced, protonated analog of nitric
oxide,9 is among the least thoroughly investigated.10 Interest in
nitroxyl has grown with the accumulation of evidence that HNO,
which has a pKa of 11.4 and exists primarily in the protonated
form under physiological conditions,9 displays important
biological roles with potential pharmacological applications
distinct from those of nitric oxide.11-13 For example, HNO reacts
directly with thiols,14 is resistant to scavenging by superoxide,15
and can activate voltage-dependent K+ channels in mammalian
vascular systems.16,17 Moreover, biochemical studies suggest that
HNO can be formed directly from nitric oxide synthase under
appropriate conditions10,18 and that NO and HNO may be able to
interconvert in the presence of superoxide dismutase (SOD).19
Despite accumulating evidence of the biological importance of
HNO, studies have been hampered by the lack of a biologically
compatible probe for the molecule. Only recently have chemical
systems capable of discerning HNO from NO been reported, but
the constructs are not suitable for work with biological
samples. |
|---|