Computational identification and experimental validation of PPRE motifs in NHE1 and MnSOD genes of Human

Computational identification and experimental validation of PPRE motifs in NHE1 and MnSOD genes of Human

Background: Activation of PPARs has been reported to inhibit the proliferation of malignant cells from different lineages. They are involved in transcription regulation of genes upon activation by a ligand. The binding of PPARs to the promoter sequence either represses or activates the gene. Henc...

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Bibliographic Details
Main Authors: Venkatachalam, Gireedhar, Kumar, Alan Prem, Yue, Loo Ser, Pervaiz, Shazib, Clement, Marie Veronique, Sakharkar, Meena Kishore
Other Authors: Singapore-MIT Alliance in Research and Technology (SMART)
Format: Article
Language:en_US
Published: BioMed Central 2011
Online Access:http://hdl.handle.net/1721.1/64781
Description
Summary:Background: Activation of PPARs has been reported to inhibit the proliferation of malignant cells from different lineages. They are involved in transcription regulation of genes upon activation by a ligand. The binding of PPARs to the promoter sequence either represses or activates the gene. Hence, PPARs represent promising targets for cancer treatment because of their anti-proliferative and pro-apoptotic activities. Here we computationally identified PPAR binding regions in NHE1 and MnSOD. We further validated the predictions in vitro. Results: Our results computationally predicted the presence of 2 PPRE motifs in NHE1 and 3 PPRE motifs in MnSOD. We experimentally confirmed the true motifs and their regulation by PPAR. Conclusion: Our results suggest that both NHE1 and MnSOD have PPRE binding motif in their upstream/promoter region and hence are regulated by PPAR upon ligand binding.

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