Computational identification and experimental validation of PPRE motifs in NHE1 and MnSOD genes of Human
Background: Activation of PPARs has been reported to inhibit the proliferation of malignant cells from different lineages. They are involved in transcription regulation of genes upon activation by a ligand. The binding of PPARs to the promoter sequence either represses or activates the gene. Henc...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | en_US |
| Published: |
BioMed Central
2011
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| Online Access: | http://hdl.handle.net/1721.1/64781 |
| _version_ | 1826205288141684736 |
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| author | Venkatachalam, Gireedhar Kumar, Alan Prem Yue, Loo Ser Pervaiz, Shazib Clement, Marie Veronique Sakharkar, Meena Kishore |
| author2 | Singapore-MIT Alliance in Research and Technology (SMART) |
| author_facet | Singapore-MIT Alliance in Research and Technology (SMART) Venkatachalam, Gireedhar Kumar, Alan Prem Yue, Loo Ser Pervaiz, Shazib Clement, Marie Veronique Sakharkar, Meena Kishore |
| author_sort | Venkatachalam, Gireedhar |
| collection | MIT |
| description | Background: Activation of PPARs has been reported to inhibit the proliferation of malignant
cells from different lineages. They are involved in transcription regulation of genes upon activation
by a ligand. The binding of PPARs to the promoter sequence either represses or activates the gene.
Hence, PPARs represent promising targets for cancer treatment because of their anti-proliferative
and pro-apoptotic activities. Here we computationally identified PPAR binding regions in NHE1 and
MnSOD. We further validated the predictions in vitro.
Results: Our results computationally predicted the presence of 2 PPRE motifs in NHE1 and 3
PPRE motifs in MnSOD. We experimentally confirmed the true motifs and their regulation by
PPAR.
Conclusion: Our results suggest that both NHE1 and MnSOD have PPRE binding motif in their
upstream/promoter region and hence are regulated by PPAR upon ligand binding. |
| first_indexed | 2024-09-23T13:10:35Z |
| format | Article |
| id | mit-1721.1/64781 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T13:10:35Z |
| publishDate | 2011 |
| publisher | BioMed Central |
| record_format | dspace |
| spelling | mit-1721.1/647812022-10-01T13:31:14Z Computational identification and experimental validation of PPRE motifs in NHE1 and MnSOD genes of Human Venkatachalam, Gireedhar Kumar, Alan Prem Yue, Loo Ser Pervaiz, Shazib Clement, Marie Veronique Sakharkar, Meena Kishore Singapore-MIT Alliance in Research and Technology (SMART) Pervaiz, Shazib Pervaiz, Shazib Background: Activation of PPARs has been reported to inhibit the proliferation of malignant cells from different lineages. They are involved in transcription regulation of genes upon activation by a ligand. The binding of PPARs to the promoter sequence either represses or activates the gene. Hence, PPARs represent promising targets for cancer treatment because of their anti-proliferative and pro-apoptotic activities. Here we computationally identified PPAR binding regions in NHE1 and MnSOD. We further validated the predictions in vitro. Results: Our results computationally predicted the presence of 2 PPRE motifs in NHE1 and 3 PPRE motifs in MnSOD. We experimentally confirmed the true motifs and their regulation by PPAR. Conclusion: Our results suggest that both NHE1 and MnSOD have PPRE binding motif in their upstream/promoter region and hence are regulated by PPAR upon ligand binding. Singapore. National Medical Research Council (Grant R-183-000-204-213) 2011-07-08T20:50:05Z 2011-07-08T20:50:05Z 2009-12 Article http://purl.org/eprint/type/JournalArticle 1471-2164 http://hdl.handle.net/1721.1/64781 Venkatachalam, Gireedhar et al. “Computational Identification and Experimental Validation of PPRE Motifs in NHE1 and MnSOD Genes of Human.” BMC Genomics 10.Suppl 3 (2009) : S5. © 2009 Venkatachalam et al; licensee BioMed Central Ltd. en_US http://dx.doi.org/10.1186/1471-2164-10-s3-s5 BMC Genomics Creative Commons Attribution http://creativecommons.org/licenses/by/2.0/ application/pdf BioMed Central BMC |
| spellingShingle | Venkatachalam, Gireedhar Kumar, Alan Prem Yue, Loo Ser Pervaiz, Shazib Clement, Marie Veronique Sakharkar, Meena Kishore Computational identification and experimental validation of PPRE motifs in NHE1 and MnSOD genes of Human |
| title | Computational identification and experimental validation of PPRE motifs in NHE1 and MnSOD genes of Human |
| title_full | Computational identification and experimental validation of PPRE motifs in NHE1 and MnSOD genes of Human |
| title_fullStr | Computational identification and experimental validation of PPRE motifs in NHE1 and MnSOD genes of Human |
| title_full_unstemmed | Computational identification and experimental validation of PPRE motifs in NHE1 and MnSOD genes of Human |
| title_short | Computational identification and experimental validation of PPRE motifs in NHE1 and MnSOD genes of Human |
| title_sort | computational identification and experimental validation of ppre motifs in nhe1 and mnsod genes of human |
| url | http://hdl.handle.net/1721.1/64781 |
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