Receptor-type guanylate cyclase is required for carbon dioxide sensation by Caenorhabditis elegans
CO2 [CO subscript 2] is both a critical regulator of animal physiology and an important sensory cue for many animals for host detection, food location, and mate finding. The free-living soil nematode Caenorhabditis elegans shows CO2 [CO subscript 2] avoidance behavior, which requires a pair of cilia...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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National Academy of Sciences (U.S.)
2011
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| Online Access: | http://hdl.handle.net/1721.1/64949 https://orcid.org/0000-0002-9964-9613 |
| _version_ | 1826206154479370240 |
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| author | Hallem, Elissa A. Spencer, W. Clay McWhirter, Rebecca D. Zeller, Georg Henz, Stefan R. Rätsch, Gunnar Miller, David M., III Sternberg, Paul W. Ringstad, Niels Horvitz, Howard Robert |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Hallem, Elissa A. Spencer, W. Clay McWhirter, Rebecca D. Zeller, Georg Henz, Stefan R. Rätsch, Gunnar Miller, David M., III Sternberg, Paul W. Ringstad, Niels Horvitz, Howard Robert |
| author_sort | Hallem, Elissa A. |
| collection | MIT |
| description | CO2 [CO subscript 2] is both a critical regulator of animal physiology and an important sensory cue for many animals for host detection, food location, and mate finding. The free-living soil nematode Caenorhabditis elegans shows CO2 [CO subscript 2] avoidance behavior, which requires a pair of ciliated sensory neurons, the BAG neurons. Using in vivo calcium imaging, we show that CO2 [CO subscript 2] specifically activates the BAG neurons and that the CO2-sensing function of BAG neurons requires TAX-2/TAX-4 cyclic nucleotide-gated ion channels and the receptor-type guanylate cyclase GCY-9. Our results delineate a molecular pathway for CO2 [CO subscript 2] sensing and suggest that activation of a receptor-type guanylate cyclase is an evolutionarily conserved mechanism by which animals detect environmental CO2 [CO subscript 2]. |
| first_indexed | 2024-09-23T13:24:57Z |
| format | Article |
| id | mit-1721.1/64949 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T13:24:57Z |
| publishDate | 2011 |
| publisher | National Academy of Sciences (U.S.) |
| record_format | dspace |
| spelling | mit-1721.1/649492022-09-28T13:59:00Z Receptor-type guanylate cyclase is required for carbon dioxide sensation by Caenorhabditis elegans Hallem, Elissa A. Spencer, W. Clay McWhirter, Rebecca D. Zeller, Georg Henz, Stefan R. Rätsch, Gunnar Miller, David M., III Sternberg, Paul W. Ringstad, Niels Horvitz, Howard Robert Massachusetts Institute of Technology. Department of Biology McGovern Institute for Brain Research at MIT Horvitz, H. Robert Horvitz, H. Robert Ringstad, Niels CO2 [CO subscript 2] is both a critical regulator of animal physiology and an important sensory cue for many animals for host detection, food location, and mate finding. The free-living soil nematode Caenorhabditis elegans shows CO2 [CO subscript 2] avoidance behavior, which requires a pair of ciliated sensory neurons, the BAG neurons. Using in vivo calcium imaging, we show that CO2 [CO subscript 2] specifically activates the BAG neurons and that the CO2-sensing function of BAG neurons requires TAX-2/TAX-4 cyclic nucleotide-gated ion channels and the receptor-type guanylate cyclase GCY-9. Our results delineate a molecular pathway for CO2 [CO subscript 2] sensing and suggest that activation of a receptor-type guanylate cyclase is an evolutionarily conserved mechanism by which animals detect environmental CO2 [CO subscript 2]. National Institutes of Health (U.S.) (Grant U01 HG004263) National Institutes of Health (U.S.) (Grant R01 NS26115) National Institutes of Health (U.S.) (Grant R01 GM24663) Helen Hay Whitney Foundation National Institutes of Health (U.S.). Pathway to Independence Award Howard Hughes Medical Institute Whitehead Institute for Biomedical Research Helen L. and Martin S. Kimmel Center for Biology and Medicine 2011-07-22T16:31:42Z 2011-07-22T16:31:42Z 2010-12 2010-08 Article http://purl.org/eprint/type/JournalArticle 0027-8424 1091-6490 http://hdl.handle.net/1721.1/64949 Hallem, E. A. et al. “Receptor-type Guanylate Cyclase Is Required for Carbon Dioxide Sensation by Caenorhabditis Elegans.” Proceedings of the National Academy of Sciences 108.1 (2010) : 254-259. https://orcid.org/0000-0002-9964-9613 en_US http://dx.doi.org/10.1073/pnas.1017354108 Proceedings of the National Academy of Sciences of the United States of America Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf National Academy of Sciences (U.S.) PNAS |
| spellingShingle | Hallem, Elissa A. Spencer, W. Clay McWhirter, Rebecca D. Zeller, Georg Henz, Stefan R. Rätsch, Gunnar Miller, David M., III Sternberg, Paul W. Ringstad, Niels Horvitz, Howard Robert Receptor-type guanylate cyclase is required for carbon dioxide sensation by Caenorhabditis elegans |
| title | Receptor-type guanylate cyclase is required for carbon dioxide sensation by Caenorhabditis elegans |
| title_full | Receptor-type guanylate cyclase is required for carbon dioxide sensation by Caenorhabditis elegans |
| title_fullStr | Receptor-type guanylate cyclase is required for carbon dioxide sensation by Caenorhabditis elegans |
| title_full_unstemmed | Receptor-type guanylate cyclase is required for carbon dioxide sensation by Caenorhabditis elegans |
| title_short | Receptor-type guanylate cyclase is required for carbon dioxide sensation by Caenorhabditis elegans |
| title_sort | receptor type guanylate cyclase is required for carbon dioxide sensation by caenorhabditis elegans |
| url | http://hdl.handle.net/1721.1/64949 https://orcid.org/0000-0002-9964-9613 |
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