Video-rate tomographic phase microscopy
Tomographic phase microscopy measures the 3-D refractive index distribution of cells and tissues by combining the information from a series of angle-dependent interferometric phase images. In the original device, the frame rate was limited to 0.1 frames per second (fps) by the technique used to ac...
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| Format: | Article |
| Language: | en_US |
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SPIE
2011
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| Online Access: | http://hdl.handle.net/1721.1/64965 https://orcid.org/0000-0001-7117-5009 https://orcid.org/0000-0002-4568-3218 |
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| author | Fang-Yen, Chris Choi, Wonshik Sung, Yongjin Holbrow, Charles J. Dasari, Ramachandra Rao Feld, Michael S. |
| author2 | Massachusetts Institute of Technology. Spectroscopy Laboratory |
| author_facet | Massachusetts Institute of Technology. Spectroscopy Laboratory Fang-Yen, Chris Choi, Wonshik Sung, Yongjin Holbrow, Charles J. Dasari, Ramachandra Rao Feld, Michael S. |
| author_sort | Fang-Yen, Chris |
| collection | MIT |
| description | Tomographic phase microscopy measures the 3-D refractive index distribution of cells and tissues
by combining the information from a series of angle-dependent interferometric phase images. In the original
device, the frame rate was limited to 0.1 frames per second (fps) by the technique used to acquire phase images,
preventing measurements of moving or rapidly changing samples. We describe an improved tomographic phase
microscope in which phase images are acquired via a spatial fringe pattern demodulation method, enabling a full
tomogram acquisition rate of 30 fps. In addition, in this system the refractive index is calculated by a diffraction
tomography algorithm that accounts for the effects of diffraction in the 3-D reconstruction.We use the instrument to
quantitatively monitor rapid changes in refractive index within defined subregions of cells due to exposure to acetic
acid or changes in medium osmolarity. |
| first_indexed | 2024-09-23T14:09:15Z |
| format | Article |
| id | mit-1721.1/64965 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T14:09:15Z |
| publishDate | 2011 |
| publisher | SPIE |
| record_format | dspace |
| spelling | mit-1721.1/649652022-09-28T18:53:33Z Video-rate tomographic phase microscopy Fang-Yen, Chris Choi, Wonshik Sung, Yongjin Holbrow, Charles J. Dasari, Ramachandra Rao Feld, Michael S. Massachusetts Institute of Technology. Spectroscopy Laboratory Dasari, Ramachandra Rao Fang-Yen, Chris Sung, Yongjin Holbrow, Charles J. Dasari, Ramachandra Rao Feld, Michael S. Tomographic phase microscopy measures the 3-D refractive index distribution of cells and tissues by combining the information from a series of angle-dependent interferometric phase images. In the original device, the frame rate was limited to 0.1 frames per second (fps) by the technique used to acquire phase images, preventing measurements of moving or rapidly changing samples. We describe an improved tomographic phase microscope in which phase images are acquired via a spatial fringe pattern demodulation method, enabling a full tomogram acquisition rate of 30 fps. In addition, in this system the refractive index is calculated by a diffraction tomography algorithm that accounts for the effects of diffraction in the 3-D reconstruction.We use the instrument to quantitatively monitor rapid changes in refractive index within defined subregions of cells due to exposure to acetic acid or changes in medium osmolarity. National Institutes of Health (U.S.) (grant P41-RR02594-18) National Science Foundation (U.S.) (grant DBI-0754339) Hamamatsu Corporation 2011-07-28T15:20:24Z 2011-07-28T15:20:24Z 2011-01 2010-06 Article http://purl.org/eprint/type/JournalArticle 1083-3668 http://hdl.handle.net/1721.1/64965 Christopher Fang-Yen, Wonshik Choi, Yongjin Sung, Charles J. Holbrow, Ramachandra R. Dasari and Michael S. Feld, "Video-rate tomographic phase microscopy", J. Biomed. Opt. 16, 011005 (Jan 14, 2011); doi:10.1117/1.3522506 © 2011 Society of Photo-Optical Instrumentation Engineers https://orcid.org/0000-0001-7117-5009 https://orcid.org/0000-0002-4568-3218 en_US http://dx.doi.org/10.1117/1.3522506 Journal of Biomedical Optics Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf SPIE SPIE |
| spellingShingle | Fang-Yen, Chris Choi, Wonshik Sung, Yongjin Holbrow, Charles J. Dasari, Ramachandra Rao Feld, Michael S. Video-rate tomographic phase microscopy |
| title | Video-rate tomographic phase microscopy |
| title_full | Video-rate tomographic phase microscopy |
| title_fullStr | Video-rate tomographic phase microscopy |
| title_full_unstemmed | Video-rate tomographic phase microscopy |
| title_short | Video-rate tomographic phase microscopy |
| title_sort | video rate tomographic phase microscopy |
| url | http://hdl.handle.net/1721.1/64965 https://orcid.org/0000-0001-7117-5009 https://orcid.org/0000-0002-4568-3218 |
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