Synthesis and Characterization of Tunable PEG - Gelatin Methacrylate Hydrogels
Poly(ethylene glycol) (PEG) hydrogels are popular for cell culture and tissue-engineering applications because they are nontoxic and exhibit favorable hydration and nutrient transport properties. However, cells cannot adhere to, remodel, proliferate within, or degrade PEG hydrogels. Methacrylated ge...
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| Format: | Article |
| Language: | en_US |
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Mary Ann Liebert
2011
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| Online Access: | http://hdl.handle.net/1721.1/64976 https://orcid.org/0000-0003-2836-5813 |
| _version_ | 1826200402749554688 |
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| author | Hutson, Che B. Nichol, Jason W. Aubin, Hug Bae, Hojae Yamanlar, Seda Al-Haque, Mohd. Shahed Koshy, Sandeep Tharian Khademhosseini, Ali |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Hutson, Che B. Nichol, Jason W. Aubin, Hug Bae, Hojae Yamanlar, Seda Al-Haque, Mohd. Shahed Koshy, Sandeep Tharian Khademhosseini, Ali |
| author_sort | Hutson, Che B. |
| collection | MIT |
| description | Poly(ethylene glycol) (PEG) hydrogels are popular for cell culture and tissue-engineering applications because they are nontoxic and exhibit favorable hydration and nutrient transport properties. However, cells cannot adhere to, remodel, proliferate within, or degrade PEG hydrogels. Methacrylated gelatin (GelMA), derived from denatured collagen, yields an enzymatically degradable, photocrosslinkable hydrogel that cells can degrade, adhere to and spread within. To combine the desirable features of each of these materials we synthesized PEG-GelMA composite hydrogels, hypothesizing that copolymerization would enable adjustable cell binding, mechanical, and degradation properties. The addition of GelMA to PEG resulted in a composite hydrogel that exhibited tunable mechanical and biological profiles. Adding GelMA (5%–15% w/v) to PEG (5% and 10% w/v) proportionally increased fibroblast surface binding and spreading as compared to PEG hydrogels (p<0.05). Encapsulated fibroblasts were also able to form 3D cellular networks 7 days after photoencapsulation only within composite hydrogels as compared to PEG alone. Additionally, PEG-GelMA hydrogels displayed tunable enzymatic degradation and stiffness profiles. PEG-GelMA composite hydrogels show great promise as tunable, cell-responsive hydrogels for 3D cell culture and regenerative medicine applications. |
| first_indexed | 2024-09-23T11:35:50Z |
| format | Article |
| id | mit-1721.1/64976 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T11:35:50Z |
| publishDate | 2011 |
| publisher | Mary Ann Liebert |
| record_format | dspace |
| spelling | mit-1721.1/649762022-10-01T04:42:11Z Synthesis and Characterization of Tunable PEG - Gelatin Methacrylate Hydrogels Hutson, Che B. Nichol, Jason W. Aubin, Hug Bae, Hojae Yamanlar, Seda Al-Haque, Mohd. Shahed Koshy, Sandeep Tharian Khademhosseini, Ali Harvard University--MIT Division of Health Sciences and Technology Khademhosseini, Ali Nichol, Jason W. Aubin, Hug Al-Haque, Mohd. Shahed Koshy, Sandeep Tharian Khademhosseini, Ali Poly(ethylene glycol) (PEG) hydrogels are popular for cell culture and tissue-engineering applications because they are nontoxic and exhibit favorable hydration and nutrient transport properties. However, cells cannot adhere to, remodel, proliferate within, or degrade PEG hydrogels. Methacrylated gelatin (GelMA), derived from denatured collagen, yields an enzymatically degradable, photocrosslinkable hydrogel that cells can degrade, adhere to and spread within. To combine the desirable features of each of these materials we synthesized PEG-GelMA composite hydrogels, hypothesizing that copolymerization would enable adjustable cell binding, mechanical, and degradation properties. The addition of GelMA to PEG resulted in a composite hydrogel that exhibited tunable mechanical and biological profiles. Adding GelMA (5%–15% w/v) to PEG (5% and 10% w/v) proportionally increased fibroblast surface binding and spreading as compared to PEG hydrogels (p<0.05). Encapsulated fibroblasts were also able to form 3D cellular networks 7 days after photoencapsulation only within composite hydrogels as compared to PEG alone. Additionally, PEG-GelMA hydrogels displayed tunable enzymatic degradation and stiffness profiles. PEG-GelMA composite hydrogels show great promise as tunable, cell-responsive hydrogels for 3D cell culture and regenerative medicine applications. National Institutes of Health (U.S.) (DE019024) National Institutes of Health (U.S.) (HL099073) National Institutes of Health (U.S.) (EB012597) National Institutes of Health (U.S.) (AR057837) National Institutes of Health (U.S.) (HL092836) National Science Foundation (U.S.) (CAREER award DMR0847287) United States. Office of Naval Research Engineer Research and Development Center (U.S.) Construction Engineering Research Laboratories (U.S.) 2011-07-28T17:25:30Z 2011-07-28T17:25:30Z 2011-04 2010-11 Article http://purl.org/eprint/type/JournalArticle 1937-3341 1937-335X http://hdl.handle.net/1721.1/64976 Hutson, Che B. et al. “Synthesis and Characterization of Tunable Poly(Ethylene Glycol): Gelatin Methacrylate Composite Hydrogels.” Tissue Engineering Part A 17.13-14 (2011) : 1713-1723. Copyright © 2011, Mary Ann Liebert, Inc. publishers https://orcid.org/0000-0003-2836-5813 en_US http://dx.doi.org/10.1089/ten.TEA.2010.0666 Tissue Engineering, Part A. Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Mary Ann Liebert Mary Ann Liebert |
| spellingShingle | Hutson, Che B. Nichol, Jason W. Aubin, Hug Bae, Hojae Yamanlar, Seda Al-Haque, Mohd. Shahed Koshy, Sandeep Tharian Khademhosseini, Ali Synthesis and Characterization of Tunable PEG - Gelatin Methacrylate Hydrogels |
| title | Synthesis and Characterization of Tunable PEG - Gelatin Methacrylate Hydrogels |
| title_full | Synthesis and Characterization of Tunable PEG - Gelatin Methacrylate Hydrogels |
| title_fullStr | Synthesis and Characterization of Tunable PEG - Gelatin Methacrylate Hydrogels |
| title_full_unstemmed | Synthesis and Characterization of Tunable PEG - Gelatin Methacrylate Hydrogels |
| title_short | Synthesis and Characterization of Tunable PEG - Gelatin Methacrylate Hydrogels |
| title_sort | synthesis and characterization of tunable peg gelatin methacrylate hydrogels |
| url | http://hdl.handle.net/1721.1/64976 https://orcid.org/0000-0003-2836-5813 |
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