Mechanism of ceroid formation in atherosclerotic plaque: in situ studies combination of Raman and fluorescence spectroscopy
Accumulation of the lipid-protein complex ceroid is a characteristic of atherosclerotic plaque. The mechanism of ceroid formation has been extensively studied, because the complex is postulated to contribute to plaque irreversibility. Despite intensive research, ceroid deposits are defined through t...
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| Format: | Article |
| Language: | en_US |
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Society of Photo-optical Instrumentation Engineers (SPIE)
2011
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| Online Access: | http://hdl.handle.net/1721.1/64981 |
| _version_ | 1826188919472914432 |
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| author | Haka, Abigail S. Kramer, John R. Dasari, Ramachandra Rao Fitzmaurice, Maryann |
| author2 | Massachusetts Institute of Technology. Spectroscopy Laboratory |
| author_facet | Massachusetts Institute of Technology. Spectroscopy Laboratory Haka, Abigail S. Kramer, John R. Dasari, Ramachandra Rao Fitzmaurice, Maryann |
| author_sort | Haka, Abigail S. |
| collection | MIT |
| description | Accumulation of the lipid-protein complex ceroid is a characteristic of atherosclerotic plaque. The mechanism of ceroid formation has been extensively studied, because the complex is postulated to contribute to plaque irreversibility. Despite intensive research, ceroid deposits are defined through their fluorescence and histochemical staining properties, while their composition remains unknown. Using Raman and fluorescence spectral microscopy, we examine the composition of ceroid in situ in aorta and coronary artery plaque. The synergy of these two types of spectroscopy allows for identification of ceroid via its fluorescence signature and elucidation of its chemical composition through the acquisition of a Raman spectrum. In accordance with in vitro predictions, low density lipoprotein (LDL) appears within the deposits primarily in its peroxidized form. The main forms of modified LDL detected in both coronary artery and aortic plaques are peroxidation products from the Fenton reaction and myeloperoxidase-hypochlorite pathway. These two peroxidation products occur in similar concentrations within the deposits and represent ∼40 and 30% of the total LDL (native and peroxidized) in the aorta and coronary artery deposits, respectively. To our knowledge, this study is the first to successfully employ Raman spectroscopy to unravel a metabolic pathway involved in disease pathogenesis: the formation of ceroid in atherosclerotic plaque. |
| first_indexed | 2024-09-23T08:07:01Z |
| format | Article |
| id | mit-1721.1/64981 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T08:07:01Z |
| publishDate | 2011 |
| publisher | Society of Photo-optical Instrumentation Engineers (SPIE) |
| record_format | dspace |
| spelling | mit-1721.1/649812022-09-23T10:59:45Z Mechanism of ceroid formation in atherosclerotic plaque: in situ studies combination of Raman and fluorescence spectroscopy Haka, Abigail S. Kramer, John R. Dasari, Ramachandra Rao Fitzmaurice, Maryann Massachusetts Institute of Technology. Spectroscopy Laboratory Dasari, Ramachandra Rao Haka, Abigail S. Kramer, John R. Dasari, Ramachandra Rao Accumulation of the lipid-protein complex ceroid is a characteristic of atherosclerotic plaque. The mechanism of ceroid formation has been extensively studied, because the complex is postulated to contribute to plaque irreversibility. Despite intensive research, ceroid deposits are defined through their fluorescence and histochemical staining properties, while their composition remains unknown. Using Raman and fluorescence spectral microscopy, we examine the composition of ceroid in situ in aorta and coronary artery plaque. The synergy of these two types of spectroscopy allows for identification of ceroid via its fluorescence signature and elucidation of its chemical composition through the acquisition of a Raman spectrum. In accordance with in vitro predictions, low density lipoprotein (LDL) appears within the deposits primarily in its peroxidized form. The main forms of modified LDL detected in both coronary artery and aortic plaques are peroxidation products from the Fenton reaction and myeloperoxidase-hypochlorite pathway. These two peroxidation products occur in similar concentrations within the deposits and represent ∼40 and 30% of the total LDL (native and peroxidized) in the aorta and coronary artery deposits, respectively. To our knowledge, this study is the first to successfully employ Raman spectroscopy to unravel a metabolic pathway involved in disease pathogenesis: the formation of ceroid in atherosclerotic plaque. National Center for Research Resources (U.S.) (Grant P41-RR-02594) Pathology Associates of University Hospitals 2011-07-28T20:00:19Z 2011-07-28T20:00:19Z 2011-01 2010-09 Article http://purl.org/eprint/type/JournalArticle 1083-3668 http://hdl.handle.net/1721.1/64981 Haka, Abigail S. et al. “Mechanism of Ceroid Formation in Atherosclerotic Plaque: In Situ Studies Using a Combination of Raman and Fluorescence Spectroscopy.” Journal of Biomedical Optics 16.1 (2011) : 011011. © 2011 Society of Photo-Optical Instrumentation Engineers (SPIE) en_US http://dx.doi.org/10.1117/1.3524304 Journal of Biomedical Optics Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Society of Photo-optical Instrumentation Engineers (SPIE) SPIE |
| spellingShingle | Haka, Abigail S. Kramer, John R. Dasari, Ramachandra Rao Fitzmaurice, Maryann Mechanism of ceroid formation in atherosclerotic plaque: in situ studies combination of Raman and fluorescence spectroscopy |
| title | Mechanism of ceroid formation in atherosclerotic plaque: in situ studies combination of Raman and fluorescence spectroscopy |
| title_full | Mechanism of ceroid formation in atherosclerotic plaque: in situ studies combination of Raman and fluorescence spectroscopy |
| title_fullStr | Mechanism of ceroid formation in atherosclerotic plaque: in situ studies combination of Raman and fluorescence spectroscopy |
| title_full_unstemmed | Mechanism of ceroid formation in atherosclerotic plaque: in situ studies combination of Raman and fluorescence spectroscopy |
| title_short | Mechanism of ceroid formation in atherosclerotic plaque: in situ studies combination of Raman and fluorescence spectroscopy |
| title_sort | mechanism of ceroid formation in atherosclerotic plaque in situ studies combination of raman and fluorescence spectroscopy |
| url | http://hdl.handle.net/1721.1/64981 |
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