Nature's approach toward ring formation and structural diversity in ergot alkaloid biosynthesis
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 2011.
| Main Author: | |
|---|---|
| Other Authors: | |
| Format: | Thesis |
| Language: | eng |
| Published: |
Massachusetts Institute of Technology
2011
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/1721.1/65472 |
| _version_ | 1811085502397284352 |
|---|---|
| author | Cheng, Johnathan Zandrew |
| author2 | Sarah E. O'Connor. |
| author_facet | Sarah E. O'Connor. Cheng, Johnathan Zandrew |
| author_sort | Cheng, Johnathan Zandrew |
| collection | MIT |
| description | Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 2011. |
| first_indexed | 2024-09-23T13:10:36Z |
| format | Thesis |
| id | mit-1721.1/65472 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T13:10:36Z |
| publishDate | 2011 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/654722019-04-10T23:05:00Z Nature's approach toward ring formation and structural diversity in ergot alkaloid biosynthesis Cheng, Johnathan Zandrew Sarah E. O'Connor. Massachusetts Institute of Technology. Dept. of Chemistry. Massachusetts Institute of Technology. Dept. of Chemistry. Chemistry. Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 2011. Vita. Cataloged from PDF version of thesis. Includes bibliographical references. Ergot alkaloids are fungal-derived secondary metabolites well known for a diverse array of pharmacological effects both beneficial and detrimental to human health. Historically, the ergot alkaloids have been known to cause ergotism in populations that consume grain contaminated by ergot alkaloid producing fungus. However, naturally isolated and semi-synthetic derivatives of certain ergot alkaloids have also been used to treat migraines, Parkinsonism, and tumor growth. Different fungal species such as Aspergillusfumigatus, Claviceps purpurea, and Neotyphodium lolii produce ergot alkaloids that have distinct structural features yet share a common tetracyclic ergoline ring scaffold. Mechanistic details regarding the formation of the common ergoline ring are not well understood, though the genes encoding the enzymes that carry out these cyclizations are believed to be well conserved across divergent fungal species. Here we describe in vivo gene disruption experiments in Aspergillusfumigatus that allowed us to identify candidate enzymes that were directly involved with the intramolecular cyclization of the ergoline ring. Additionally, we discuss the cloning and heterologous expression of these genes to further characterize their catalytic function. Old Yellow Enzyme homologues from the ergot gene clusters of Aspergillus fumigatus and Neotyphodium loii were shown to catalyze the formation of the D ring of the ergoline skeleton. These enzymes catalyzed either reductase or isomerase type reactions to yield distinct pathway intermediates. Mutational analysis was used to engineer an Old Yellow Enzyme that displayed both reductase and isomerase activities, thereby elucidating the mechanistic basis behind this switch in enzymatic activity. These findings present a mechanistic rationale behind nature's biosynthetic strategy toward ring cyclization and the introduction of structural diversity into the ergot alkaloid class of natural products. by Johnathan Zandrew Cheng. Ph.D. 2011-08-30T15:39:21Z 2011-08-30T15:39:21Z 2011 2011 Thesis http://hdl.handle.net/1721.1/65472 743301033 eng M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 232 p. application/pdf Massachusetts Institute of Technology |
| spellingShingle | Chemistry. Cheng, Johnathan Zandrew Nature's approach toward ring formation and structural diversity in ergot alkaloid biosynthesis |
| title | Nature's approach toward ring formation and structural diversity in ergot alkaloid biosynthesis |
| title_full | Nature's approach toward ring formation and structural diversity in ergot alkaloid biosynthesis |
| title_fullStr | Nature's approach toward ring formation and structural diversity in ergot alkaloid biosynthesis |
| title_full_unstemmed | Nature's approach toward ring formation and structural diversity in ergot alkaloid biosynthesis |
| title_short | Nature's approach toward ring formation and structural diversity in ergot alkaloid biosynthesis |
| title_sort | nature s approach toward ring formation and structural diversity in ergot alkaloid biosynthesis |
| topic | Chemistry. |
| url | http://hdl.handle.net/1721.1/65472 |
| work_keys_str_mv | AT chengjohnathanzandrew naturesapproachtowardringformationandstructuraldiversityinergotalkaloidbiosynthesis |