Structural studies of metalloenzyme complexes in acetogenic carbon fixation
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 2011.
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| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2011
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| Online Access: | http://hdl.handle.net/1721.1/65474 |
| _version_ | 1826194540442157056 |
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| author | Kung, Yan |
| author2 | Catherine L. Drennan. |
| author_facet | Catherine L. Drennan. Kung, Yan |
| author_sort | Kung, Yan |
| collection | MIT |
| description | Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 2011. |
| first_indexed | 2024-09-23T09:57:20Z |
| format | Thesis |
| id | mit-1721.1/65474 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T09:57:20Z |
| publishDate | 2011 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/654742022-01-13T07:54:21Z Structural studies of metalloenzyme complexes in acetogenic carbon fixation Kung, Yan Catherine L. Drennan. Massachusetts Institute of Technology. Dept. of Chemistry. Massachusetts Institute of Technology. Department of Chemistry Chemistry. Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 2011. Vita. Cataloged from PDF version of thesis. Includes bibliographical references. Acetogenic bacteria use the Wood-Ljungdahl carbon fixation pathway to produce cellular carbon from CO₂. This process requires several metalloenzymes that employ transition metals such as iron, nickel, and cobalt towards the production of acetyl-CoA, the final product. In one stage of the pathway, the cobalt-containing B₁₂ cofactor harbored by the corrinoid iron-sulfur protein (CFeSP) transfers a methyl group from methyltetrahydrofolate (CH₃-H₄folate), which is bound by a methyltransferase enzyme (MeTr), to a nickel-containing metallocluster called the A-cluster of the downstream enzyme, acetyl-CoA synthase (ACS). Such B12-dependent methyl transfer reactions require the construction of large, multimodular enzyme complexes whose threedimensional assemblies are, at present, largely uncharacterized. X-ray crystallography was used to solve the structure of a CFeSP/MeTr complex, the first crystal structure of a B12-dependent methyltransferase to depict all protein domains required for B12 binding, activation, protection, and catalysis. This structure, along with in crystallo activity data, illustrates how conformational movements, which can occur within protein crystals, enable the B12 cofactor to alternate between a sequestered conformation for cofactor protection and an active conformation for catalysis. Small-angle X-ray scattering (SAXS) experiments were also conducted to explore the quaternary composition of the complex in solution and revealed that multiple CFeSP/MeTr complexes can be formed. In another reaction of the Wood-Ljungdahl carbon fixation pathway, a nickel and iron containing metallocluster called the C-cluster of carbon monoxide dehydrogenase (CODH) reduces a second molecule of CO₂ to CO, an intermediate that is channeled to the ACS A-cluster. Although the structure of the C-cluster was first described a decade ago, its catalytic mechanism remained unresolved. To provide mechanistic insight into the chemistry employed at the C-cluster, crystal structures were determined with substrate and inhibitor molecules bound to the C-cluster of the CODH/ACS complex. These structures capture states of the C-cluster at key steps in the reaction and contribute to a consensus model for C-cluster chemistry. With structural descriptions for both CFeSP/MeTr and CODH/ACS complexes, this work has illuminated the molecular details for metalloenzyme complex assembly and catalysis in the acetogenic Wood-Ljungdahl carbon fixation pathway. by Yan Kung. Ph.D. 2011-08-30T15:39:36Z 2011-08-30T15:39:36Z 2011 2011 Thesis http://hdl.handle.net/1721.1/65474 746507963 eng M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 288 p. application/pdf Massachusetts Institute of Technology |
| spellingShingle | Chemistry. Kung, Yan Structural studies of metalloenzyme complexes in acetogenic carbon fixation |
| title | Structural studies of metalloenzyme complexes in acetogenic carbon fixation |
| title_full | Structural studies of metalloenzyme complexes in acetogenic carbon fixation |
| title_fullStr | Structural studies of metalloenzyme complexes in acetogenic carbon fixation |
| title_full_unstemmed | Structural studies of metalloenzyme complexes in acetogenic carbon fixation |
| title_short | Structural studies of metalloenzyme complexes in acetogenic carbon fixation |
| title_sort | structural studies of metalloenzyme complexes in acetogenic carbon fixation |
| topic | Chemistry. |
| url | http://hdl.handle.net/1721.1/65474 |
| work_keys_str_mv | AT kungyan structuralstudiesofmetalloenzymecomplexesinacetogeniccarbonfixation |