Coupling among growth rate response, metabolic cycle, and cell division cycle in yeast
We studied the steady-state responses to changes in growth rate of yeast when ethanol is the sole source of carbon and energy. Analysis of these data, together with data from studies where glucose was the carbon source, allowed us to distinguish a “universal” growth rate response (GRR) common to all...
| Main Authors: | , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
American Society for Cell Biology
2011
|
| Online Access: | http://hdl.handle.net/1721.1/65834 https://orcid.org/0000-0003-2035-1820 |
| _version_ | 1826202158689681408 |
|---|---|
| author | Slavov, Nikolai G. Botstein, David |
| author2 | MIT Materials Research Laboratory |
| author_facet | MIT Materials Research Laboratory Slavov, Nikolai G. Botstein, David |
| author_sort | Slavov, Nikolai G. |
| collection | MIT |
| description | We studied the steady-state responses to changes in growth rate of yeast when ethanol is the sole source of carbon and energy. Analysis of these data, together with data from studies where glucose was the carbon source, allowed us to distinguish a “universal” growth rate response (GRR) common to all media studied from a GRR specific to the carbon source. Genes with positive universal GRR include ribosomal, translation, and mitochondrial genes, and those with negative GRR include autophagy, vacuolar, and stress response genes. The carbon source–specific GRR genes control mitochondrial function, peroxisomes, and synthesis of vitamins and cofactors, suggesting this response may reflect the intensity of oxidative metabolism. All genes with universal GRR, which comprise 25% of the genome, are expressed periodically in the yeast metabolic cycle (YMC). We propose that the universal GRR may be accounted for by changes in the relative durations of the YMC phases. This idea is supported by oxygen consumption data from metabolically synchronized cultures with doubling times ranging from 5 to 14 h. We found that the high oxygen consumption phase of the YMC can coincide exactly with the S phase of the cell division cycle, suggesting that oxidative metabolism and DNA replication are not incompatible. |
| first_indexed | 2024-09-23T12:02:47Z |
| format | Article |
| id | mit-1721.1/65834 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T12:02:47Z |
| publishDate | 2011 |
| publisher | American Society for Cell Biology |
| record_format | dspace |
| spelling | mit-1721.1/658342022-10-01T07:49:09Z Coupling among growth rate response, metabolic cycle, and cell division cycle in yeast Slavov, Nikolai G. Botstein, David MIT Materials Research Laboratory Slavov, Nikolai G. Slavov, Nikolai G. We studied the steady-state responses to changes in growth rate of yeast when ethanol is the sole source of carbon and energy. Analysis of these data, together with data from studies where glucose was the carbon source, allowed us to distinguish a “universal” growth rate response (GRR) common to all media studied from a GRR specific to the carbon source. Genes with positive universal GRR include ribosomal, translation, and mitochondrial genes, and those with negative GRR include autophagy, vacuolar, and stress response genes. The carbon source–specific GRR genes control mitochondrial function, peroxisomes, and synthesis of vitamins and cofactors, suggesting this response may reflect the intensity of oxidative metabolism. All genes with universal GRR, which comprise 25% of the genome, are expressed periodically in the yeast metabolic cycle (YMC). We propose that the universal GRR may be accounted for by changes in the relative durations of the YMC phases. This idea is supported by oxygen consumption data from metabolically synchronized cultures with doubling times ranging from 5 to 14 h. We found that the high oxygen consumption phase of the YMC can coincide exactly with the S phase of the cell division cycle, suggesting that oxidative metabolism and DNA replication are not incompatible. National Institutes of Health (U.S.) (GM046406) National Institute of General Medical Sciences (U.S.). Center for Quantitative Biology (GM071508) 2011-09-14T15:31:04Z 2011-09-14T15:31:04Z 2011-06 2011-04 Article http://purl.org/eprint/type/JournalArticle 1059-1524 1939-4586 http://hdl.handle.net/1721.1/65834 Slavov, N., and D. Botstein. “Coupling among growth rate response, metabolic cycle, and cell division cycle in yeast.” Molecular Biology of the Cell 22 (2011): 1997-2009. https://orcid.org/0000-0003-2035-1820 en_US http://dx.doi.org/10.1091/mbc.E11-02-0132 Molecular Biology of the Cell Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf American Society for Cell Biology ASCB |
| spellingShingle | Slavov, Nikolai G. Botstein, David Coupling among growth rate response, metabolic cycle, and cell division cycle in yeast |
| title | Coupling among growth rate response, metabolic cycle, and cell division cycle in yeast |
| title_full | Coupling among growth rate response, metabolic cycle, and cell division cycle in yeast |
| title_fullStr | Coupling among growth rate response, metabolic cycle, and cell division cycle in yeast |
| title_full_unstemmed | Coupling among growth rate response, metabolic cycle, and cell division cycle in yeast |
| title_short | Coupling among growth rate response, metabolic cycle, and cell division cycle in yeast |
| title_sort | coupling among growth rate response metabolic cycle and cell division cycle in yeast |
| url | http://hdl.handle.net/1721.1/65834 https://orcid.org/0000-0003-2035-1820 |
| work_keys_str_mv | AT slavovnikolaig couplingamonggrowthrateresponsemetaboliccycleandcelldivisioncycleinyeast AT botsteindavid couplingamonggrowthrateresponsemetaboliccycleandcelldivisioncycleinyeast |