Rapid Estimation of Binding Activity of Influenza Virus Hemagglutinin to Human and Avian Receptors
A critical step for avian influenza viruses to infect human hosts and cause epidemics or pandemics is acquisition of the ability of the viral hemagglutinin (HA) to bind to human receptors. However, current global influenza surveillance does not monitor HA binding specificity due to a lack of rapid a...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Public Library of Science
2011
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| Online Access: | http://hdl.handle.net/1721.1/65932 https://orcid.org/0000-0002-5687-6154 |
| _version_ | 1826216235055972352 |
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| author | Cao, Yang Koh, Xiaoying Dong, Libo Du, Xiangjun Wu, Aiping Ding, Xilai Deng, Hongyu Shu, Yuelong Chen, Jianzhu Jiang, Taijiao |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Cao, Yang Koh, Xiaoying Dong, Libo Du, Xiangjun Wu, Aiping Ding, Xilai Deng, Hongyu Shu, Yuelong Chen, Jianzhu Jiang, Taijiao |
| author_sort | Cao, Yang |
| collection | MIT |
| description | A critical step for avian influenza viruses to infect human hosts and cause epidemics or pandemics is acquisition of the ability of the viral hemagglutinin (HA) to bind to human receptors. However, current global influenza surveillance does not monitor HA binding specificity due to a lack of rapid and reliable assays. Here we report a computational method that uses an effective scoring function to quantify HA-receptor binding activities with high accuracy and speed. Application of this method reveals receptor specificity changes and its temporal relationship with antigenicity changes during the evolution of human H3N2 viruses. The method predicts that two amino acid differences at 222 and 225 between HAs of A/Fujian/411/02 and A/Panama/2007/99 viruses account for their differences in binding to both avian and human receptors; this prediction was verified experimentally. The new computational method could provide an urgently needed tool for rapid and large-scale analysis of HA receptor specificities for global influenza surveillance. |
| first_indexed | 2024-09-23T16:44:26Z |
| format | Article |
| id | mit-1721.1/65932 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T16:44:26Z |
| publishDate | 2011 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | mit-1721.1/659322022-10-03T07:56:15Z Rapid Estimation of Binding Activity of Influenza Virus Hemagglutinin to Human and Avian Receptors Cao, Yang Koh, Xiaoying Dong, Libo Du, Xiangjun Wu, Aiping Ding, Xilai Deng, Hongyu Shu, Yuelong Chen, Jianzhu Jiang, Taijiao Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Biology Chen, Jianzhu Koh, Xiaoying Chen, Jianzhu A critical step for avian influenza viruses to infect human hosts and cause epidemics or pandemics is acquisition of the ability of the viral hemagglutinin (HA) to bind to human receptors. However, current global influenza surveillance does not monitor HA binding specificity due to a lack of rapid and reliable assays. Here we report a computational method that uses an effective scoring function to quantify HA-receptor binding activities with high accuracy and speed. Application of this method reveals receptor specificity changes and its temporal relationship with antigenicity changes during the evolution of human H3N2 viruses. The method predicts that two amino acid differences at 222 and 225 between HAs of A/Fujian/411/02 and A/Panama/2007/99 viruses account for their differences in binding to both avian and human receptors; this prediction was verified experimentally. The new computational method could provide an urgently needed tool for rapid and large-scale analysis of HA receptor specificities for global influenza surveillance. National Key Project (2008ZX10004-013) National Institutes of Health (U.S.) (grant AI07443) Singapore-MIT Alliance for Research and Technology Massachusetts Institute of Technology. International Science and Technology Initiatives Global Seed Fund National Basic Research Program (973 Program) (2009CB918503) National Basic Research Program (973 Program) (2006CB911002) 2011-09-22T14:09:53Z 2011-09-22T14:09:53Z 2011-04 2010-12 Article http://purl.org/eprint/type/JournalArticle 1932-6203 http://hdl.handle.net/1721.1/65932 Cao, Yang et al. “Rapid Estimation of Binding Activity of Influenza Virus Hemagglutinin to Human and Avian Receptors.” Ed. Christian Schönbach. PLoS ONE 6.4 (2011) : e18664. https://orcid.org/0000-0002-5687-6154 en_US http://dx.doi.org/10.1371/journal.pone.0018664 PLoS One Creative Commons Attribution http://creativecommons.org/licenses/by/2.5/ application/pdf Public Library of Science PLoS |
| spellingShingle | Cao, Yang Koh, Xiaoying Dong, Libo Du, Xiangjun Wu, Aiping Ding, Xilai Deng, Hongyu Shu, Yuelong Chen, Jianzhu Jiang, Taijiao Rapid Estimation of Binding Activity of Influenza Virus Hemagglutinin to Human and Avian Receptors |
| title | Rapid Estimation of Binding Activity of Influenza Virus Hemagglutinin to Human and Avian Receptors |
| title_full | Rapid Estimation of Binding Activity of Influenza Virus Hemagglutinin to Human and Avian Receptors |
| title_fullStr | Rapid Estimation of Binding Activity of Influenza Virus Hemagglutinin to Human and Avian Receptors |
| title_full_unstemmed | Rapid Estimation of Binding Activity of Influenza Virus Hemagglutinin to Human and Avian Receptors |
| title_short | Rapid Estimation of Binding Activity of Influenza Virus Hemagglutinin to Human and Avian Receptors |
| title_sort | rapid estimation of binding activity of influenza virus hemagglutinin to human and avian receptors |
| url | http://hdl.handle.net/1721.1/65932 https://orcid.org/0000-0002-5687-6154 |
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