Dynamic Imaging of the Effector Immune Response to Listeria Infection In Vivo
Host defense against the intracellular pathogen Listeria monocytogenes (Lm) requires innate and adaptive immunity. Here, we directly imaged immune cell dynamics at Lm foci established by dendritic cells in the subcapsular red pulp (scDC) using intravital microscopy. Blood borne Lm rapidly associated...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Public Library of Science
2011
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| Online Access: | http://hdl.handle.net/1721.1/66113 |
| _version_ | 1826215172573757440 |
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| author | Waite, Janelle C. Leiner, Ingrid Lauer, Peter Rae, Chris S. Barbet, Gaetan Zheng, Huan Portnoy, Daniel A. Pamer, Eric G. Dustin, Michael L. |
| author2 | Massachusetts Institute of Technology. Department of Chemical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Chemical Engineering Waite, Janelle C. Leiner, Ingrid Lauer, Peter Rae, Chris S. Barbet, Gaetan Zheng, Huan Portnoy, Daniel A. Pamer, Eric G. Dustin, Michael L. |
| author_sort | Waite, Janelle C. |
| collection | MIT |
| description | Host defense against the intracellular pathogen Listeria monocytogenes (Lm) requires innate and adaptive immunity. Here, we directly imaged immune cell dynamics at Lm foci established by dendritic cells in the subcapsular red pulp (scDC) using intravital microscopy. Blood borne Lm rapidly associated with scDC. Myelomonocytic cells (MMC) swarmed around non-motile scDC forming foci from which blood flow was excluded. The depletion of scDC after foci were established resulted in a 10-fold reduction in viable Lm, while graded depletion of MMC resulted in 30–1000 fold increase in viable Lm in foci with enhanced blood flow. Effector CD8+ [CD8 superscript +] T cells at sites of infection displayed a two-tiered reduction in motility with antigen independent and antigen dependent components, including stable interactions with infected and non-infected scDC. Thus, swarming MMC contribute to control of Lm prior to development of T cell immunity by direct killing and sequestration from blood flow, while scDC appear to promote Lm survival while preferentially interacting with CD8+ [CD8 superscript +] T cells in effector sites. |
| first_indexed | 2024-09-23T16:17:44Z |
| format | Article |
| id | mit-1721.1/66113 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T16:17:44Z |
| publishDate | 2011 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | mit-1721.1/661132022-09-29T19:26:39Z Dynamic Imaging of the Effector Immune Response to Listeria Infection In Vivo Waite, Janelle C. Leiner, Ingrid Lauer, Peter Rae, Chris S. Barbet, Gaetan Zheng, Huan Portnoy, Daniel A. Pamer, Eric G. Dustin, Michael L. Massachusetts Institute of Technology. Department of Chemical Engineering Zheng, Huan Zheng, Huan Host defense against the intracellular pathogen Listeria monocytogenes (Lm) requires innate and adaptive immunity. Here, we directly imaged immune cell dynamics at Lm foci established by dendritic cells in the subcapsular red pulp (scDC) using intravital microscopy. Blood borne Lm rapidly associated with scDC. Myelomonocytic cells (MMC) swarmed around non-motile scDC forming foci from which blood flow was excluded. The depletion of scDC after foci were established resulted in a 10-fold reduction in viable Lm, while graded depletion of MMC resulted in 30–1000 fold increase in viable Lm in foci with enhanced blood flow. Effector CD8+ [CD8 superscript +] T cells at sites of infection displayed a two-tiered reduction in motility with antigen independent and antigen dependent components, including stable interactions with infected and non-infected scDC. Thus, swarming MMC contribute to control of Lm prior to development of T cell immunity by direct killing and sequestration from blood flow, while scDC appear to promote Lm survival while preferentially interacting with CD8+ [CD8 superscript +] T cells in effector sites. National Institutes of Health (U.S.) (Grant P01AI-071195) 2011-09-28T21:00:20Z 2011-09-28T21:00:20Z 2011-03 2010-08 Article http://purl.org/eprint/type/JournalArticle 1553-7366 1553-7374 http://hdl.handle.net/1721.1/66113 Waite, Janelle C. et al. “Dynamic Imaging of the Effector Immune Response to Listeria Infection In Vivo.” Ed. David S. Schneider. PLoS Pathogens 7 (3) (2011): e1001326. © 2011 Waite et al. en_US http://dx.doi.org/10.1371/journal.ppat.1001326 PLoS Pathogens Creative Commons Attribution http://creativecommons.org/licenses/by/2.5/ application/pdf Public Library of Science PLoS |
| spellingShingle | Waite, Janelle C. Leiner, Ingrid Lauer, Peter Rae, Chris S. Barbet, Gaetan Zheng, Huan Portnoy, Daniel A. Pamer, Eric G. Dustin, Michael L. Dynamic Imaging of the Effector Immune Response to Listeria Infection In Vivo |
| title | Dynamic Imaging of the Effector Immune Response to Listeria Infection In Vivo |
| title_full | Dynamic Imaging of the Effector Immune Response to Listeria Infection In Vivo |
| title_fullStr | Dynamic Imaging of the Effector Immune Response to Listeria Infection In Vivo |
| title_full_unstemmed | Dynamic Imaging of the Effector Immune Response to Listeria Infection In Vivo |
| title_short | Dynamic Imaging of the Effector Immune Response to Listeria Infection In Vivo |
| title_sort | dynamic imaging of the effector immune response to listeria infection in vivo |
| url | http://hdl.handle.net/1721.1/66113 |
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