In-Silico Patterning of Vascular Mesenchymal Cells in Three Dimensions
Cells organize in complex three-dimensional patterns by interacting with proteins along with the surrounding extracellular matrix. This organization provides the mechanical and chemical cues that ultimately influence a cell's differentiation and function. Here, we computationally investigate th...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | en_US |
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Public Library of Science
2011
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| Online Access: | http://hdl.handle.net/1721.1/66267 https://orcid.org/0000-0002-1293-2097 |
| _version_ | 1811077909958361088 |
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| author | Danino, Tal Volfson, Dmitri Bhatia, Sangeeta N. Tsimring, Lev Hasty, Jeff |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Danino, Tal Volfson, Dmitri Bhatia, Sangeeta N. Tsimring, Lev Hasty, Jeff |
| author_sort | Danino, Tal |
| collection | MIT |
| description | Cells organize in complex three-dimensional patterns by interacting with proteins along with the surrounding extracellular matrix. This organization provides the mechanical and chemical cues that ultimately influence a cell's differentiation and function. Here, we computationally investigate the pattern formation process of vascular mesenchymal cells arising from their interaction with Bone Morphogenic Protein-2 (BMP-2) and its inhibitor, Matrix Gla Protein (MGP). Using a first-principles approach, we derive a reaction-diffusion model based on the biochemical interactions of BMP-2, MGP and cells. Simulations of the model exhibit a wide variety of three-dimensional patterns not observed in a two-dimensional analysis. We demonstrate the emergence of three types of patterns: spheres, tubes, and sheets, and show that the patterns can be tuned by modifying parameters in the model such as the degradation rates of proteins and chemotactic coefficient of cells. Our model may be useful for improved engineering of three-dimensional tissue structures as well as for understanding three dimensional microenvironments in developmental processes. |
| first_indexed | 2024-09-23T10:50:13Z |
| format | Article |
| id | mit-1721.1/66267 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T10:50:13Z |
| publishDate | 2011 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | mit-1721.1/662672022-09-30T23:21:36Z In-Silico Patterning of Vascular Mesenchymal Cells in Three Dimensions Danino, Tal Volfson, Dmitri Bhatia, Sangeeta N. Tsimring, Lev Hasty, Jeff Harvard University--MIT Division of Health Sciences and Technology Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Bhatia, Sangeeta N. Bhatia, Sangeeta N. Cells organize in complex three-dimensional patterns by interacting with proteins along with the surrounding extracellular matrix. This organization provides the mechanical and chemical cues that ultimately influence a cell's differentiation and function. Here, we computationally investigate the pattern formation process of vascular mesenchymal cells arising from their interaction with Bone Morphogenic Protein-2 (BMP-2) and its inhibitor, Matrix Gla Protein (MGP). Using a first-principles approach, we derive a reaction-diffusion model based on the biochemical interactions of BMP-2, MGP and cells. Simulations of the model exhibit a wide variety of three-dimensional patterns not observed in a two-dimensional analysis. We demonstrate the emergence of three types of patterns: spheres, tubes, and sheets, and show that the patterns can be tuned by modifying parameters in the model such as the degradation rates of proteins and chemotactic coefficient of cells. Our model may be useful for improved engineering of three-dimensional tissue structures as well as for understanding three dimensional microenvironments in developmental processes. National Institutes of Health (U.S.) (GM69811) United States. Dept. of Energy (DOE CSGF fellowship) 2011-10-17T14:33:20Z 2011-10-17T14:33:20Z 2011-05 2010-10 Article http://purl.org/eprint/type/JournalArticle 1932-6203 http://hdl.handle.net/1721.1/66267 Danino, Tal et al. “In-Silico Patterning of Vascular Mesenchymal Cells in Three Dimensions.” Ed. Mukund Thattai. PLoS ONE 6 (2011): e20182. https://orcid.org/0000-0002-1293-2097 en_US http://dx.doi.org/10.1371/journal.pone.0020182 PLoS ONE Creative Commons Attribution http://creativecommons.org/licenses/by/2.5/ application/pdf Public Library of Science PLoS |
| spellingShingle | Danino, Tal Volfson, Dmitri Bhatia, Sangeeta N. Tsimring, Lev Hasty, Jeff In-Silico Patterning of Vascular Mesenchymal Cells in Three Dimensions |
| title | In-Silico Patterning of Vascular Mesenchymal Cells in Three Dimensions |
| title_full | In-Silico Patterning of Vascular Mesenchymal Cells in Three Dimensions |
| title_fullStr | In-Silico Patterning of Vascular Mesenchymal Cells in Three Dimensions |
| title_full_unstemmed | In-Silico Patterning of Vascular Mesenchymal Cells in Three Dimensions |
| title_short | In-Silico Patterning of Vascular Mesenchymal Cells in Three Dimensions |
| title_sort | in silico patterning of vascular mesenchymal cells in three dimensions |
| url | http://hdl.handle.net/1721.1/66267 https://orcid.org/0000-0002-1293-2097 |
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