Effects of 810-nm Laser on Murine Bone-Marrow-Derived Dendritic Cells
Objective: The purpose of this study was to Investigate the effect of 810-nm low level laser therapy (LLLT) on dendritic cells (DC) in vitro. Background data: LLLT can enhance wound healing and increase cell proliferation and survival, and is used to treat inflammatory conditions. However there are...
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| Format: | Article |
| Language: | en_US |
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Mary Ann Liebert, Inc.
2011
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| Online Access: | http://hdl.handle.net/1721.1/66561 |
| _version_ | 1811074786624798720 |
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| author | Chen, Aaron Chih-Hao Huang, Ying-Ying Sharma, Sulbha K. Hamblin, Michael R. |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Chen, Aaron Chih-Hao Huang, Ying-Ying Sharma, Sulbha K. Hamblin, Michael R. |
| author_sort | Chen, Aaron Chih-Hao |
| collection | MIT |
| description | Objective: The purpose of this study was to Investigate the effect of 810-nm low level laser therapy (LLLT) on dendritic cells (DC) in vitro. Background data: LLLT can enhance wound healing and increase cell proliferation and survival, and is used to treat inflammatory conditions. However there are reports that LLLT can stimulate leukocytes and could therefore be pro-inflammatory. Recently, DC have been found to play an important role in inflammation and immune response. Methods: Murine bone-marrow-derived DC were isolated, stimulated with lipopolysaccharide (LPS) or CpG oligodeoxynucleotide and treated with 810-nm laser, using fluences of 0.3, 3, and 30 J/cm2 delivered at irradiances of 1, 10, and 100 mW/cm2 respectively. Confocal microscopy, flow cytometry for DC markers, viability using propidium iodide, enzyme-linked immunosorbent assays (ELISA) for secreted interleukin-12 (IL-12), and bioluminescence measurements in cells transduced with a reporter for toll-like receptor (TLR)-9/nuclear factor kappa B (NF-κB) activation, were performed. Results: LLLT changed the morphology of LPS-stimulated DC, increased their viability, and altered the balance of DC activation markers (major histocompatibility complex [MHC] class 2 up and CD86 down). LLLT reduced IL-12 secretion from DC stimulated by either LPS or CpG. LLLT reduced NF-κB activation in reporter cells stimulated with CpG. There was no obvious light dose response observed. Conclusions: Taken together, these data suggest that 810-nm LLLT has an anti-inflammatory effect on activated DC, possibly mediated by cyclic adenosine monophosphate (cAMP) and reduced NF-κB signaling. |
| first_indexed | 2024-09-23T09:55:29Z |
| format | Article |
| id | mit-1721.1/66561 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T09:55:29Z |
| publishDate | 2011 |
| publisher | Mary Ann Liebert, Inc. |
| record_format | dspace |
| spelling | mit-1721.1/665612022-09-30T17:43:16Z Effects of 810-nm Laser on Murine Bone-Marrow-Derived Dendritic Cells Chen, Aaron Chih-Hao Huang, Ying-Ying Sharma, Sulbha K. Hamblin, Michael R. Harvard University--MIT Division of Health Sciences and Technology Hamblin, Michael R. Hamblin, Michael R. Objective: The purpose of this study was to Investigate the effect of 810-nm low level laser therapy (LLLT) on dendritic cells (DC) in vitro. Background data: LLLT can enhance wound healing and increase cell proliferation and survival, and is used to treat inflammatory conditions. However there are reports that LLLT can stimulate leukocytes and could therefore be pro-inflammatory. Recently, DC have been found to play an important role in inflammation and immune response. Methods: Murine bone-marrow-derived DC were isolated, stimulated with lipopolysaccharide (LPS) or CpG oligodeoxynucleotide and treated with 810-nm laser, using fluences of 0.3, 3, and 30 J/cm2 delivered at irradiances of 1, 10, and 100 mW/cm2 respectively. Confocal microscopy, flow cytometry for DC markers, viability using propidium iodide, enzyme-linked immunosorbent assays (ELISA) for secreted interleukin-12 (IL-12), and bioluminescence measurements in cells transduced with a reporter for toll-like receptor (TLR)-9/nuclear factor kappa B (NF-κB) activation, were performed. Results: LLLT changed the morphology of LPS-stimulated DC, increased their viability, and altered the balance of DC activation markers (major histocompatibility complex [MHC] class 2 up and CD86 down). LLLT reduced IL-12 secretion from DC stimulated by either LPS or CpG. LLLT reduced NF-κB activation in reporter cells stimulated with CpG. There was no obvious light dose response observed. Conclusions: Taken together, these data suggest that 810-nm LLLT has an anti-inflammatory effect on activated DC, possibly mediated by cyclic adenosine monophosphate (cAMP) and reduced NF-κB signaling. National Institutes of Health (U.S.) (NIH grant R01AI050875) Center for Integration of Medicine and Innovative Technology (DAMD17-02-2-0006) United States. Dept. of Defense. Congressionally Directed Medical Research Programs (TBI, W81XWH-09-1-0514) United States. Air Force Office of Scientific Research (FA9950-04-1-0079) 2011-10-24T20:18:13Z 2011-10-24T20:18:13Z 2011-06 Article http://purl.org/eprint/type/JournalArticle 1549-5418 1557-8550 http://hdl.handle.net/1721.1/66561 Chen, Aaron C.-H. et al. “Effects of 810-nm Laser on Murine Bone-Marrow-Derived Dendritic Cells.” Photomedicine and Laser Surgery 29 (2011): 383-389. ©2011 Mary Ann Liebert, Inc. en_US http://dx.doi.org/10.1089/pho.2010.2837 Photomedicine and Laser Surgery Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Mary Ann Liebert, Inc. Mary Ann Liebert |
| spellingShingle | Chen, Aaron Chih-Hao Huang, Ying-Ying Sharma, Sulbha K. Hamblin, Michael R. Effects of 810-nm Laser on Murine Bone-Marrow-Derived Dendritic Cells |
| title | Effects of 810-nm Laser on Murine Bone-Marrow-Derived Dendritic Cells |
| title_full | Effects of 810-nm Laser on Murine Bone-Marrow-Derived Dendritic Cells |
| title_fullStr | Effects of 810-nm Laser on Murine Bone-Marrow-Derived Dendritic Cells |
| title_full_unstemmed | Effects of 810-nm Laser on Murine Bone-Marrow-Derived Dendritic Cells |
| title_short | Effects of 810-nm Laser on Murine Bone-Marrow-Derived Dendritic Cells |
| title_sort | effects of 810 nm laser on murine bone marrow derived dendritic cells |
| url | http://hdl.handle.net/1721.1/66561 |
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