A Secreted Enzyme Reporter System for MRI
An important goal in modern biology is to understand how molecular processes commonly studied at the cellular level give rise to physiological functions in complex tissues and organisms. Non-invasive imaging of gene-expression patterns in whole animals could provide information critical to this end,...
| Main Authors: | , , |
|---|---|
| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
John Wiley & Sons, Inc.
2011
|
| Online Access: | http://hdl.handle.net/1721.1/66597 https://orcid.org/0000-0002-2834-6359 |
| _version_ | 1826212079030239232 |
|---|---|
| author | Durocher, Yves Westmeyer, Gil Gregor Jasanoff, Alan Pradip |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Durocher, Yves Westmeyer, Gil Gregor Jasanoff, Alan Pradip |
| author_sort | Durocher, Yves |
| collection | MIT |
| description | An important goal in modern biology is to understand how molecular processes commonly studied at the cellular level give rise to physiological functions in complex tissues and organisms. Non-invasive imaging of gene-expression patterns in whole animals could provide information critical to this end, but current methods lack sensitivity and spatiotemporal precision. Enzymatic reporter systems detectable by magnetic resonance imaging (MRI) address these limitations by combining the relatively high spatial and temporal resolution of MRI with the ability of each genetically expressed enzyme to generate many MRI-detectable product molecules.1, 2 A challenge with the imaging-based detection of some of the most popular reporter enzymes is the need to deliver MRI probes to their sites of action within cells. Herein we describe a new reporter-gene system for MRI that relieves this problem by harnessing an extracellular enzyme, the mammalian secreted alkaline phosphatase (SEAP). |
| first_indexed | 2024-09-23T15:15:46Z |
| format | Article |
| id | mit-1721.1/66597 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T15:15:46Z |
| publishDate | 2011 |
| publisher | John Wiley & Sons, Inc. |
| record_format | dspace |
| spelling | mit-1721.1/665972022-10-02T01:45:38Z A Secreted Enzyme Reporter System for MRI Durocher, Yves Westmeyer, Gil Gregor Jasanoff, Alan Pradip Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Massachusetts Institute of Technology. Department of Nuclear Science and Engineering Jasanoff, Alan Pradip Westmeyer, Gil Gregor Jasanoff, Alan Pradip An important goal in modern biology is to understand how molecular processes commonly studied at the cellular level give rise to physiological functions in complex tissues and organisms. Non-invasive imaging of gene-expression patterns in whole animals could provide information critical to this end, but current methods lack sensitivity and spatiotemporal precision. Enzymatic reporter systems detectable by magnetic resonance imaging (MRI) address these limitations by combining the relatively high spatial and temporal resolution of MRI with the ability of each genetically expressed enzyme to generate many MRI-detectable product molecules.1, 2 A challenge with the imaging-based detection of some of the most popular reporter enzymes is the need to deliver MRI probes to their sites of action within cells. Herein we describe a new reporter-gene system for MRI that relieves this problem by harnessing an extracellular enzyme, the mammalian secreted alkaline phosphatase (SEAP). National Institutes of Health (U.S.) (grant DP2-OD002114) 2011-10-26T21:07:47Z 2011-10-26T21:07:47Z 2010-04 Article http://purl.org/eprint/type/JournalArticle 0570-0833 http://hdl.handle.net/1721.1/66597 Westmeyer, Gil G., Yves Durocher, and Alan Jasanoff. “A Secreted Enzyme Reporter System for MRI.” Angewandte Chemie International Edition (2010): NA-NA. Web. 26 Oct. 2011. © 2010 John Wiley & Sons, Inc. https://orcid.org/0000-0002-2834-6359 en_US http://dx.doi.org/10.1002/anie.200906712 Angewandte Chemie International Edition Creative Commons Attribution-Noncommercial-Share Alike 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf John Wiley & Sons, Inc. PubMed Central |
| spellingShingle | Durocher, Yves Westmeyer, Gil Gregor Jasanoff, Alan Pradip A Secreted Enzyme Reporter System for MRI |
| title | A Secreted Enzyme Reporter System for MRI |
| title_full | A Secreted Enzyme Reporter System for MRI |
| title_fullStr | A Secreted Enzyme Reporter System for MRI |
| title_full_unstemmed | A Secreted Enzyme Reporter System for MRI |
| title_short | A Secreted Enzyme Reporter System for MRI |
| title_sort | secreted enzyme reporter system for mri |
| url | http://hdl.handle.net/1721.1/66597 https://orcid.org/0000-0002-2834-6359 |
| work_keys_str_mv | AT durocheryves asecretedenzymereportersystemformri AT westmeyergilgregor asecretedenzymereportersystemformri AT jasanoffalanpradip asecretedenzymereportersystemformri AT durocheryves secretedenzymereportersystemformri AT westmeyergilgregor secretedenzymereportersystemformri AT jasanoffalanpradip secretedenzymereportersystemformri |