Improved Detergent-Based Recovery of Polyhydroxyalkanoates (Phas).
Extracting polyhydroxyalkanoate (PHA) polymer from bacterial cells often involves harsh conditions, including use of environmentally harmful solvents. We evaluated different detergents under various conditions to extract PHA from Ralstonia eutropha and Escherichia coli cells. Most detergents t...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Springer
2011
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| Online Access: | http://hdl.handle.net/1721.1/66917 https://orcid.org/0000-0002-1015-1270 https://orcid.org/0000-0002-6671-5987 |
| _version_ | 1826203044733255680 |
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| author | Yang, Yung-Hun Brigham, Christopher J. Willis, Laura B. Rha, ChoKyun Sinskey, Anthony J. |
| author2 | Harvard University--MIT Division of Health Sciences and Technology |
| author_facet | Harvard University--MIT Division of Health Sciences and Technology Yang, Yung-Hun Brigham, Christopher J. Willis, Laura B. Rha, ChoKyun Sinskey, Anthony J. |
| author_sort | Yang, Yung-Hun |
| collection | MIT |
| description | Extracting polyhydroxyalkanoate (PHA)
polymer from bacterial cells often involves harsh
conditions, including use of environmentally harmful
solvents. We evaluated different detergents under
various conditions to extract PHA from Ralstonia
eutropha and Escherichia coli cells. Most detergents
tested recovered highly pure PHA polymer from cells
in amounts that depended on the percentage of polymer present in the cell. Detergents such as linear
alkylbenzene sulfonic acid (LAS-99) produced a high
yield of high purity polymer, and less detergent was
needed compared to the amount of SDS to produce
comparable yields. LAS-99 also has the advantage of
being biodegradable and environmentally safe.
Chemical extraction of PHA with detergents could
potentially minimize or eliminate the need to use
harsh organic solvents, thus making industrial PHA
production a cleaner technology process. |
| first_indexed | 2024-09-23T12:30:49Z |
| format | Article |
| id | mit-1721.1/66917 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T12:30:49Z |
| publishDate | 2011 |
| publisher | Springer |
| record_format | dspace |
| spelling | mit-1721.1/669172022-09-28T08:11:38Z Improved Detergent-Based Recovery of Polyhydroxyalkanoates (Phas). Yang, Yung-Hun Brigham, Christopher J. Willis, Laura B. Rha, ChoKyun Sinskey, Anthony J. Harvard University--MIT Division of Health Sciences and Technology Massachusetts Institute of Technology. Department of Biology Massachusetts Institute of Technology. Engineering Systems Division Sinskey, Anthony J. Sinskey, Anthony J. Brigham, Christopher J. Willis, Laura Rha, ChoKyun Sinskey, Anthony J. Extracting polyhydroxyalkanoate (PHA) polymer from bacterial cells often involves harsh conditions, including use of environmentally harmful solvents. We evaluated different detergents under various conditions to extract PHA from Ralstonia eutropha and Escherichia coli cells. Most detergents tested recovered highly pure PHA polymer from cells in amounts that depended on the percentage of polymer present in the cell. Detergents such as linear alkylbenzene sulfonic acid (LAS-99) produced a high yield of high purity polymer, and less detergent was needed compared to the amount of SDS to produce comparable yields. LAS-99 also has the advantage of being biodegradable and environmentally safe. Chemical extraction of PHA with detergents could potentially minimize or eliminate the need to use harsh organic solvents, thus making industrial PHA production a cleaner technology process. Malaysian Office of Science, Technology and Innovation 2011-11-03T19:19:21Z 2011-11-03T19:19:21Z 2011-01 Article http://purl.org/eprint/type/JournalArticle 1573-6776 0141-5492 http://hdl.handle.net/1721.1/66917 Yang, Yung-Hun et al. “Improved detergent-based recovery of polyhydroxyalkanoates (PHAs).” Biotechnology Letters 33 (2011): 937-942. Web. 3 Nov. 2011. © 2011 Springer https://orcid.org/0000-0002-1015-1270 https://orcid.org/0000-0002-6671-5987 en_US http://dx.doi.org/10.1007/s10529-010-0513-4 Biotechnology Letters Creative Commons Attribution-Noncommercial-Share Alike 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf Springer Sinskey |
| spellingShingle | Yang, Yung-Hun Brigham, Christopher J. Willis, Laura B. Rha, ChoKyun Sinskey, Anthony J. Improved Detergent-Based Recovery of Polyhydroxyalkanoates (Phas). |
| title | Improved Detergent-Based Recovery of Polyhydroxyalkanoates (Phas). |
| title_full | Improved Detergent-Based Recovery of Polyhydroxyalkanoates (Phas). |
| title_fullStr | Improved Detergent-Based Recovery of Polyhydroxyalkanoates (Phas). |
| title_full_unstemmed | Improved Detergent-Based Recovery of Polyhydroxyalkanoates (Phas). |
| title_short | Improved Detergent-Based Recovery of Polyhydroxyalkanoates (Phas). |
| title_sort | improved detergent based recovery of polyhydroxyalkanoates phas |
| url | http://hdl.handle.net/1721.1/66917 https://orcid.org/0000-0002-1015-1270 https://orcid.org/0000-0002-6671-5987 |
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