Mechanical robustness of Pseudomonas aeruginosa biofilms
Biofilms grow on various surfaces and in many different environments, a phenomenon that constitutes major problems in industry and medicine. Despite their importance little is known about the viscoelastic properties of biofilms and how these depend on the chemical microenvironment. Here, we find tha...
| Auteurs principaux: | , , , |
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| Autres auteurs: | |
| Format: | Article |
| Langue: | en_US |
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Royal Society of Chemistry
2011
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| Accès en ligne: | http://hdl.handle.net/1721.1/67039 |
| _version_ | 1826192190933565440 |
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| author | Lieleg, Oliver Caldara, Marina Baumgartel, Regina Ribbeck, Katharina |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Lieleg, Oliver Caldara, Marina Baumgartel, Regina Ribbeck, Katharina |
| author_sort | Lieleg, Oliver |
| collection | MIT |
| description | Biofilms grow on various surfaces and in many different environments, a phenomenon that constitutes major problems in industry and medicine. Despite their importance little is known about the viscoelastic properties of biofilms and how these depend on the chemical microenvironment. Here, we find that the mechanical properties of Pseudomonas aeruginosa (P.a.) biofilms are highly robust towards chemical perturbations. Specifically, we observe that P.a. biofilms are able to fully regain their initial stiffness after yielding is enforced, even in the presence of chemicals. Moreover, only trivalent ions and citric acid significantly affect the biofilm elasticity, the first of which also alters the texture of the material. Finally, our results indicate that biofilm mechanics and bacteria viability inside the biofilm are not necessarily linked which suggests that targeting bacteria alone might not be sufficient for biofilm removal strategies. |
| first_indexed | 2024-09-23T09:07:38Z |
| format | Article |
| id | mit-1721.1/67039 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T09:07:38Z |
| publishDate | 2011 |
| publisher | Royal Society of Chemistry |
| record_format | dspace |
| spelling | mit-1721.1/670392022-09-26T10:39:45Z Mechanical robustness of Pseudomonas aeruginosa biofilms Lieleg, Oliver Caldara, Marina Baumgartel, Regina Ribbeck, Katharina Massachusetts Institute of Technology. Department of Biological Engineering Ribbeck, Katherina Lieleg, Oliver Caldara, Marina Baumgartel, Regina Grodzinsky, Katharina Biofilms grow on various surfaces and in many different environments, a phenomenon that constitutes major problems in industry and medicine. Despite their importance little is known about the viscoelastic properties of biofilms and how these depend on the chemical microenvironment. Here, we find that the mechanical properties of Pseudomonas aeruginosa (P.a.) biofilms are highly robust towards chemical perturbations. Specifically, we observe that P.a. biofilms are able to fully regain their initial stiffness after yielding is enforced, even in the presence of chemicals. Moreover, only trivalent ions and citric acid significantly affect the biofilm elasticity, the first of which also alters the texture of the material. Finally, our results indicate that biofilm mechanics and bacteria viability inside the biofilm are not necessarily linked which suggests that targeting bacteria alone might not be sufficient for biofilm removal strategies. National Institute of Mental Health (U.S.) (P50-GM068763) National Institute of Mental Health (U.S.) (P30-ES002109) German Academic Exchange Service (DAAD) 2011-11-16T21:18:40Z 2011-11-16T21:18:40Z 2011-02 2010-12 Article http://purl.org/eprint/type/JournalArticle 1744-6848 1744-683X http://hdl.handle.net/1721.1/67039 Lieleg, Oliver et al. “Mechanical robustness of Pseudomonasaeruginosa biofilms.” Soft Matter 7 (2011): 3307. Web. 16 Nov. 2011. © 2011 Royal Society of Chemistry en_US http://dx.doi.org/10.1039/c0sm01467b Soft Matter Creative Commons Attribution-Noncommercial-Share Alike 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf Royal Society of Chemistry PubMed Central |
| spellingShingle | Lieleg, Oliver Caldara, Marina Baumgartel, Regina Ribbeck, Katharina Mechanical robustness of Pseudomonas aeruginosa biofilms |
| title | Mechanical robustness of Pseudomonas aeruginosa biofilms |
| title_full | Mechanical robustness of Pseudomonas aeruginosa biofilms |
| title_fullStr | Mechanical robustness of Pseudomonas aeruginosa biofilms |
| title_full_unstemmed | Mechanical robustness of Pseudomonas aeruginosa biofilms |
| title_short | Mechanical robustness of Pseudomonas aeruginosa biofilms |
| title_sort | mechanical robustness of pseudomonas aeruginosa biofilms |
| url | http://hdl.handle.net/1721.1/67039 |
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