Cell biomechanics of the central nervous system
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biological Engineering, 2011.
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| Other Authors: | |
| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2011
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| Online Access: | http://hdl.handle.net/1721.1/67202 |
| _version_ | 1811077764254531584 |
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| author | Bernick, Kristin Briana |
| author2 | Simona Socrate and Roger Kamm. |
| author_facet | Simona Socrate and Roger Kamm. Bernick, Kristin Briana |
| author_sort | Bernick, Kristin Briana |
| collection | MIT |
| description | Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biological Engineering, 2011. |
| first_indexed | 2024-09-23T10:48:02Z |
| format | Thesis |
| id | mit-1721.1/67202 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T10:48:02Z |
| publishDate | 2011 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/672022019-04-14T07:08:32Z Cell biomechanics of the central nervous system Bernick, Kristin Briana Simona Socrate and Roger Kamm. Massachusetts Institute of Technology. Dept. of Biological Engineering. Massachusetts Institute of Technology. Dept. of Biological Engineering. Biological Engineering. Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biological Engineering, 2011. Cataloged from PDF version of thesis. Includes bibliographical references (p. 133-153). Traumatic brain injury (TBI) is a significant cause of death and morbidity in both the civilian and military populations. The major causes of TBI, such as motor vehicle accidents, falls, sports concussions, and ballistic and explosive blast threats for military personnel, are well established and extensively characterized; however, there remains much to be learned about the specific mechanisms of damage leading to brain injury, especially at the cellular level. In order to understand how cells of the central nervous system (CNS) respond to mechanical insults and stimuli, a combined modeling/experimental approach was adopted. A computational framework was developed to accurately model how cells deform under various macroscopically imposed loading conditions. In addition, in vitro (cell culture) models were established to investigate damage responses to biologically relevant mechanical insults. In order to develop computational models of cell response to mechanical loading, it is essential to have accurate material properties for all cells of interest. In this work, the mechanical responses of neurons and astrocytes were quantified using atomic force microscopy (AFM) at three different loading rates and under relaxation to enable characterization of both the elastic and viscous components of the cell response. AFM data were used to calibrate an eight-parameter rheological model implemented in the framework of a commercial finite element package (Abaqus). Model parameters fit to the measured responses of neurons and astrocytes provide a quantitative measure of homogenized nonlinear viscoelastic properties for each cell type. In order to ensure that the measured responses could be considered representative of cell populations in their physiological environment, cells were also grown and tested on substrates of various stiffness, with the softest substrate mimicking the stiffness of brain tissue. Results of this study showed both the morphology and measured force response of astrocytes to be significantly affected by the stiffness of their substrate, with cells becoming increasingly rounded on soft substrates. Results of simulations suggested that changes in cell morphology were able to account for the observed changes in AFM force response, without significant changes to the cell material properties. In contrast, no significant changes in cell morphology were observed for neurons. These results highlight the importance of growing cells in a biologically relevant environment when studying mechanically mediated responses, such as TBI. To address this requirement, we developed two model systems with CNS cells grown in soft, 3D gels to investigate damage arising from dynamic compressive loading and from a shock pressure wave. These damage protocols, coupled with the single cell computational models, provide a new tool set for characterizing damage mechanisms in CNS cells and for studying TBI in highly controllable in vitro conditions. by Kristin Briana Bernick. Ph.D. 2011-11-18T21:00:06Z 2011-11-18T21:00:06Z 2011 2011 Thesis http://hdl.handle.net/1721.1/67202 758862907 eng MIT theses are protected by copyright. They may be viewed, downloaded, or printed from this source but further reproduction or distribution in any format is prohibited without written permission. http://dspace.mit.edu/handle/1721.1/7582 153 p. application/pdf Massachusetts Institute of Technology |
| spellingShingle | Biological Engineering. Bernick, Kristin Briana Cell biomechanics of the central nervous system |
| title | Cell biomechanics of the central nervous system |
| title_full | Cell biomechanics of the central nervous system |
| title_fullStr | Cell biomechanics of the central nervous system |
| title_full_unstemmed | Cell biomechanics of the central nervous system |
| title_short | Cell biomechanics of the central nervous system |
| title_sort | cell biomechanics of the central nervous system |
| topic | Biological Engineering. |
| url | http://hdl.handle.net/1721.1/67202 |
| work_keys_str_mv | AT bernickkristinbriana cellbiomechanicsofthecentralnervoussystem |