K-ras Mutation Targeted to Gastric Tissue Progenitor Cells Results in Chronic Inflammation, an Altered Microenvironment, and Progression to Intraepithelial
Chronic infectious diseases, such as Helicobacter pylori infection, can promote cancer in a large part through induction of chronic inflammation. Oncogenic K-ras mutation in epithelial cells activates inflammatory pathways, which could compensate for a lack of infectious stimulus. Gastric histopatho...
| Main Authors: | , , , , , , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
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American Association for Cancer Research
2011
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| Online Access: | http://hdl.handle.net/1721.1/67285 https://orcid.org/0000-0001-9307-6116 |
| _version_ | 1811078544700211200 |
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| author | Okumura, Tomoyuki Ericksen, Russell E. Takaishi, Shigeo Wang, Sophie S. W. Dubeykovskaya, Zinaida Shibata, Wataru Betz, Kelly S. Muthupalani, Sureshkumar Rogers, Arlin B. Fox, James G. Rustgi, Anil K. Wang, Timothy C. |
| author2 | Massachusetts Institute of Technology. Division of Comparative Medicine |
| author_facet | Massachusetts Institute of Technology. Division of Comparative Medicine Okumura, Tomoyuki Ericksen, Russell E. Takaishi, Shigeo Wang, Sophie S. W. Dubeykovskaya, Zinaida Shibata, Wataru Betz, Kelly S. Muthupalani, Sureshkumar Rogers, Arlin B. Fox, James G. Rustgi, Anil K. Wang, Timothy C. |
| author_sort | Okumura, Tomoyuki |
| collection | MIT |
| description | Chronic infectious diseases, such as Helicobacter pylori infection, can promote cancer in a large part through induction of chronic inflammation. Oncogenic K-ras mutation in epithelial cells activates inflammatory pathways, which could compensate for a lack of infectious stimulus. Gastric histopathology and putative progenitor markers [doublecortin and calcium/calmodulin-dependent protein kinase-like 1 (Dcamkl1) and keratin 19 (K19)] in K19-K-ras-V12 (K19-kras) transgenic mice were assessed at 3, 6, 12, and 18 months of age, in comparison with Helicobacter felis–infected wild-type littermates. Inflammation was evaluated by reverse transcription–PCR of proinflammatory cytokines, and K19-kras mice were transplanted with green fluorescent protein (GFP)–labeled bone marrow. Both H. felis infection and K-ras mutation induced upregulation of proinflammatory cytokines, expansion of Dcamkl1+ cells, and progression to oxyntic atrophy, metaplasia, hyperplasia, and high-grade dysplasia. K19-kras transgenic mice uniquely displayed mucous metaplasia as early as 3 months and progressed to high-grade dysplasia and invasive intramucosal carcinoma by 20 months. In bone marrow–transplanted K19-kras mice that progressed to dysplasia, a large proportion of stromal cells were GFP+ and bone marrow–derived, but only rare GFP+ epithelial cells were observed. GFP+ bone marrow–derived cells included leukocytes and CD45− stromal cells that expressed vimentin or α smooth muscle actin and were often found surrounding clusters of Dcamkl1+ cells at the base of gastric glands. In conclusion, the expression of mutant K-ras in K19+ gastric epithelial cells can induce chronic inflammation and promote the development of dysplasia. |
| first_indexed | 2024-09-23T11:01:47Z |
| format | Article |
| id | mit-1721.1/67285 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T11:01:47Z |
| publishDate | 2011 |
| publisher | American Association for Cancer Research |
| record_format | dspace |
| spelling | mit-1721.1/672852022-10-01T00:37:26Z K-ras Mutation Targeted to Gastric Tissue Progenitor Cells Results in Chronic Inflammation, an Altered Microenvironment, and Progression to Intraepithelial Okumura, Tomoyuki Ericksen, Russell E. Takaishi, Shigeo Wang, Sophie S. W. Dubeykovskaya, Zinaida Shibata, Wataru Betz, Kelly S. Muthupalani, Sureshkumar Rogers, Arlin B. Fox, James G. Rustgi, Anil K. Wang, Timothy C. Massachusetts Institute of Technology. Division of Comparative Medicine Fox, James G. Muthupalani, Sureshkumar Fox, James G. Chronic infectious diseases, such as Helicobacter pylori infection, can promote cancer in a large part through induction of chronic inflammation. Oncogenic K-ras mutation in epithelial cells activates inflammatory pathways, which could compensate for a lack of infectious stimulus. Gastric histopathology and putative progenitor markers [doublecortin and calcium/calmodulin-dependent protein kinase-like 1 (Dcamkl1) and keratin 19 (K19)] in K19-K-ras-V12 (K19-kras) transgenic mice were assessed at 3, 6, 12, and 18 months of age, in comparison with Helicobacter felis–infected wild-type littermates. Inflammation was evaluated by reverse transcription–PCR of proinflammatory cytokines, and K19-kras mice were transplanted with green fluorescent protein (GFP)–labeled bone marrow. Both H. felis infection and K-ras mutation induced upregulation of proinflammatory cytokines, expansion of Dcamkl1+ cells, and progression to oxyntic atrophy, metaplasia, hyperplasia, and high-grade dysplasia. K19-kras transgenic mice uniquely displayed mucous metaplasia as early as 3 months and progressed to high-grade dysplasia and invasive intramucosal carcinoma by 20 months. In bone marrow–transplanted K19-kras mice that progressed to dysplasia, a large proportion of stromal cells were GFP+ and bone marrow–derived, but only rare GFP+ epithelial cells were observed. GFP+ bone marrow–derived cells included leukocytes and CD45− stromal cells that expressed vimentin or α smooth muscle actin and were often found surrounding clusters of Dcamkl1+ cells at the base of gastric glands. In conclusion, the expression of mutant K-ras in K19+ gastric epithelial cells can induce chronic inflammation and promote the development of dysplasia. National Institutes of Health (U.S.) (Grant NIH 5R01 CA120979-02) National Institutes of Health (U.S.) (Grant R01 DK060694) National Institutes of Health (U.S.) (Grant U01 CA143056) National Institutes of Health (U.S.) (Grant P30 DK050306) Uehara Memorial Foundation 2011-11-21T21:57:31Z 2011-11-21T21:57:31Z 2010-11 2010-08 Article http://purl.org/eprint/type/JournalArticle 0008-5472 1538-7445 http://hdl.handle.net/1721.1/67285 Okumura, T. et al. “K-ras Mutation Targeted to Gastric Tissue Progenitor Cells Results in Chronic Inflammation, an Altered Microenvironment, and Progression to Intraepithelial Neoplasia.” Cancer Research 70 (2010): 8435-8445. https://orcid.org/0000-0001-9307-6116 en_US http://dx.doi.org/10.1158/0008-5472.can-10-1506 Cancer Research Creative Commons Attribution-Noncommercial-Share Alike 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf American Association for Cancer Research PubMed Central |
| spellingShingle | Okumura, Tomoyuki Ericksen, Russell E. Takaishi, Shigeo Wang, Sophie S. W. Dubeykovskaya, Zinaida Shibata, Wataru Betz, Kelly S. Muthupalani, Sureshkumar Rogers, Arlin B. Fox, James G. Rustgi, Anil K. Wang, Timothy C. K-ras Mutation Targeted to Gastric Tissue Progenitor Cells Results in Chronic Inflammation, an Altered Microenvironment, and Progression to Intraepithelial |
| title | K-ras Mutation Targeted to Gastric Tissue Progenitor Cells Results in Chronic Inflammation, an Altered Microenvironment, and Progression to Intraepithelial |
| title_full | K-ras Mutation Targeted to Gastric Tissue Progenitor Cells Results in Chronic Inflammation, an Altered Microenvironment, and Progression to Intraepithelial |
| title_fullStr | K-ras Mutation Targeted to Gastric Tissue Progenitor Cells Results in Chronic Inflammation, an Altered Microenvironment, and Progression to Intraepithelial |
| title_full_unstemmed | K-ras Mutation Targeted to Gastric Tissue Progenitor Cells Results in Chronic Inflammation, an Altered Microenvironment, and Progression to Intraepithelial |
| title_short | K-ras Mutation Targeted to Gastric Tissue Progenitor Cells Results in Chronic Inflammation, an Altered Microenvironment, and Progression to Intraepithelial |
| title_sort | k ras mutation targeted to gastric tissue progenitor cells results in chronic inflammation an altered microenvironment and progression to intraepithelial |
| url | http://hdl.handle.net/1721.1/67285 https://orcid.org/0000-0001-9307-6116 |
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