An improved method for large-scale preparation of negatively and positively supercoiled plasmid DNA
A rigorous understanding of the biological function of superhelical tension in cellular DNA requires the development of new tools and model systems for study. To this end, an ethidium bromide–free method has been developed to prepare large quantities of either negatively or positively super-coiled p...
Main Authors: | Barth, Marita C., Dederich, Debra A., Dedon, Peter C. |
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Other Authors: | Massachusetts Institute of Technology. Center for Environmental Health Sciences |
Format: | Article |
Language: | en_US |
Published: |
Informa Healthcare
2011
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Online Access: | http://hdl.handle.net/1721.1/67309 https://orcid.org/0000-0003-0011-3067 |
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