An improved method for large-scale preparation of negatively and positively supercoiled plasmid DNA
A rigorous understanding of the biological function of superhelical tension in cellular DNA requires the development of new tools and model systems for study. To this end, an ethidium bromide–free method has been developed to prepare large quantities of either negatively or positively super-coiled p...
| Autori principali: | , , |
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| Natura: | Articolo |
| Lingua: | en_US |
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Informa Healthcare
2011
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| Accesso online: | http://hdl.handle.net/1721.1/67309 https://orcid.org/0000-0003-0011-3067 |