Microarchitecture of Three-Dimensional Scaffolds Influences Cell Migration Behavior via Junction Interactions
Cell migration plays a critical role in a wide variety of physiological and pathological phenomena as well as in scaffold-based tissue engineering. Cell migration behavior is known to be governed by biochemical stimuli and cellular interactions. Biophysical processes associated with interactions bet...
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| Format: | Article |
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Elsevier B.V. for the Biophysical Society
2011
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| Online Access: | http://hdl.handle.net/1721.1/67449 https://orcid.org/0000-0003-0151-708X https://orcid.org/0000-0001-5458-154X https://orcid.org/0000-0001-7559-7815 |
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| author | Harley, Brendan A. C. Kim, Hyung-Do Zaman, Muhammad H. Yannas, Ioannis V. Lauffenburger, Douglas A. Gibson, Lorna |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Harley, Brendan A. C. Kim, Hyung-Do Zaman, Muhammad H. Yannas, Ioannis V. Lauffenburger, Douglas A. Gibson, Lorna |
| author_sort | Harley, Brendan A. C. |
| collection | MIT |
| description | Cell migration plays a critical role in a wide variety of physiological and pathological phenomena as well as in scaffold-based tissue engineering. Cell migration behavior is known to be governed by biochemical stimuli and cellular interactions. Biophysical processes associated with interactions between the cell and its surrounding extracellular matrix may also play a significant role in regulating migration. Although biophysical properties of two-dimensional substrates have been shown to significantly influence cell migration, elucidating factors governing migration in a three-dimensional environment is a relatively new avenue of research. Here, we investigate the effect of the three-dimensional microstructure, specifically the pore size and Young's modulus, of collagen-glycosaminoglycan scaffolds on the migratory behavior of individual mouse fibroblasts. We observe that the fibroblast migration, characterized by motile fraction as well as locomotion speed, decreases as scaffold pore size increases across a range from 90 to 150 μm. Directly testing the effects of varying strut Young's modulus on cell motility showed a biphasic relationship between cell speed and strut modulus and also indicated that mechanical factors were not responsible for the observed effect of scaffold pore size on cell motility. Instead, in-depth analysis of cell locomotion paths revealed that the distribution of junction points between scaffold struts strongly modulates motility. Strut junction interactions affect local directional persistence as well as cell speed at and away from the junctions, providing a new biophysical mechanism for the governance of cell motility by the extracellular microstructure. |
| first_indexed | 2024-09-23T12:32:40Z |
| format | Article |
| id | mit-1721.1/67449 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T12:32:40Z |
| publishDate | 2011 |
| publisher | Elsevier B.V. for the Biophysical Society |
| record_format | dspace |
| spelling | mit-1721.1/674492022-10-01T09:39:44Z Microarchitecture of Three-Dimensional Scaffolds Influences Cell Migration Behavior via Junction Interactions Harley, Brendan A. C. Kim, Hyung-Do Zaman, Muhammad H. Yannas, Ioannis V. Lauffenburger, Douglas A. Gibson, Lorna Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Materials Science and Engineering Massachusetts Institute of Technology. Department of Mechanical Engineering Gibson, Lorna Harley, Brendan A. C. Kim, Hyung-Do Yannas, Ioannis V. Lauffenburger, Douglas A. Gibson, Lorna Cell migration plays a critical role in a wide variety of physiological and pathological phenomena as well as in scaffold-based tissue engineering. Cell migration behavior is known to be governed by biochemical stimuli and cellular interactions. Biophysical processes associated with interactions between the cell and its surrounding extracellular matrix may also play a significant role in regulating migration. Although biophysical properties of two-dimensional substrates have been shown to significantly influence cell migration, elucidating factors governing migration in a three-dimensional environment is a relatively new avenue of research. Here, we investigate the effect of the three-dimensional microstructure, specifically the pore size and Young's modulus, of collagen-glycosaminoglycan scaffolds on the migratory behavior of individual mouse fibroblasts. We observe that the fibroblast migration, characterized by motile fraction as well as locomotion speed, decreases as scaffold pore size increases across a range from 90 to 150 μm. Directly testing the effects of varying strut Young's modulus on cell motility showed a biphasic relationship between cell speed and strut modulus and also indicated that mechanical factors were not responsible for the observed effect of scaffold pore size on cell motility. Instead, in-depth analysis of cell locomotion paths revealed that the distribution of junction points between scaffold struts strongly modulates motility. Strut junction interactions affect local directional persistence as well as cell speed at and away from the junctions, providing a new biophysical mechanism for the governance of cell motility by the extracellular microstructure. National Institute of General Medical Sciences (U.S.) (Cell Migration Consortium NIH U54-GM064346) Cambridge-MIT Institute Massachusetts Institute of Technology. Dept. of Materials Science and Engineering (Matoula S. Salapatas Professorship) Massachusetts Institute of Technology (Whitaker Health Science Fund Fellowship) Sokol Foundation 2011-12-05T22:05:53Z 2011-12-05T22:05:53Z 2008-10 2007-09 Article http://purl.org/eprint/type/JournalArticle 0006-3495 1542-0086 http://hdl.handle.net/1721.1/67449 Harley, Brendan A.C. et al. “Microarchitecture of Three-Dimensional Scaffolds Influences Cell Migration Behavior via Junction Interactions.” Biophysical Journal 95.8 (2008): 4013-4024.© 2008 The Biophysical Society. https://orcid.org/0000-0003-0151-708X https://orcid.org/0000-0001-5458-154X https://orcid.org/0000-0001-7559-7815 en_US http://dx.doi.org/10.1529/biophysj.107.122598 Biophysical Journal Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf Elsevier B.V. for the Biophysical Society Biophysical Society |
| spellingShingle | Harley, Brendan A. C. Kim, Hyung-Do Zaman, Muhammad H. Yannas, Ioannis V. Lauffenburger, Douglas A. Gibson, Lorna Microarchitecture of Three-Dimensional Scaffolds Influences Cell Migration Behavior via Junction Interactions |
| title | Microarchitecture of Three-Dimensional Scaffolds Influences Cell Migration Behavior via Junction Interactions |
| title_full | Microarchitecture of Three-Dimensional Scaffolds Influences Cell Migration Behavior via Junction Interactions |
| title_fullStr | Microarchitecture of Three-Dimensional Scaffolds Influences Cell Migration Behavior via Junction Interactions |
| title_full_unstemmed | Microarchitecture of Three-Dimensional Scaffolds Influences Cell Migration Behavior via Junction Interactions |
| title_short | Microarchitecture of Three-Dimensional Scaffolds Influences Cell Migration Behavior via Junction Interactions |
| title_sort | microarchitecture of three dimensional scaffolds influences cell migration behavior via junction interactions |
| url | http://hdl.handle.net/1721.1/67449 https://orcid.org/0000-0003-0151-708X https://orcid.org/0000-0001-5458-154X https://orcid.org/0000-0001-7559-7815 |
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