Contributions to the analysis of proteins
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2011.
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| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2011
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| Online Access: | http://hdl.handle.net/1721.1/67599 |
| _version_ | 1826205895676133376 |
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| author | Sharifi Sedeh, Reza |
| author2 | Klaus-Jürgen Bathe and Mark Bathe. |
| author_facet | Klaus-Jürgen Bathe and Mark Bathe. Sharifi Sedeh, Reza |
| author_sort | Sharifi Sedeh, Reza |
| collection | MIT |
| description | Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2011. |
| first_indexed | 2024-09-23T13:20:45Z |
| format | Thesis |
| id | mit-1721.1/67599 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T13:20:45Z |
| publishDate | 2011 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/675992019-04-12T12:45:48Z Contributions to the analysis of proteins Sharifi Sedeh, Reza Klaus-Jürgen Bathe and Mark Bathe. Massachusetts Institute of Technology. Dept. of Mechanical Engineering. Massachusetts Institute of Technology. Dept. of Mechanical Engineering. Mechanical Engineering. Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2011. Cataloged from PDF version of thesis. Includes bibliographical references (p. 131-145). Proteins are essential to organisms and play a central role in almost every biological process. The analysis of the conformational dynamics and mechanics of proteins using numerical methods, such as normal mode analysis (NMA), provides insight into their functional mechanisms. However, despite the fact that much effort has been focused on improving NMA over the last few decades, the analysis of large-scale protein motions is still infeasible due to computational limitations. In this work, first, we identify the usefulness and effectiveness of the subspace iteration (SSI) procedure, otherwise widely used in structural engineering, for the analysis of proteins. We also develop a novel technique for the selection of iteration vectors in protein NMA, which significantly increases the effectiveness of the method. The SSI procedure also lends itself naturally to efficient NMA of multiple neighboring macromolecular conformations, as demonstrated in a conformational change pathway analysis of adenylate kinase. Next, we present a new algorithm to account for the effects of solvent-damping on slow protein conformational dynamics. The algorithm proves to be an effective approach to calculating the diffusion coefficients of proteins with various molecular weights, as well as their Langevin modes and corresponding relaxation times, as demonstrated for the small molecule crambin. Finally, the structure of Homo sapiens fascin-1, an actin-binding protein that is present predominantly in filopodia, is examined and described in detail. Application of a sequence conservation analysis to the protein indicates highly conserved surface patches near the putative actin-binding domains of fascin. A novel conformational dynamics analysis suggests that these domains are coupled via an allosteric mechanism that may have important functional implications for F-actin bundling by fascin. by Reza Sharifi Sedeh. Ph.D. 2011-12-09T21:30:16Z 2011-12-09T21:30:16Z 2011 2011 Thesis http://hdl.handle.net/1721.1/67599 764449130 eng M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 145 p. application/pdf Massachusetts Institute of Technology |
| spellingShingle | Mechanical Engineering. Sharifi Sedeh, Reza Contributions to the analysis of proteins |
| title | Contributions to the analysis of proteins |
| title_full | Contributions to the analysis of proteins |
| title_fullStr | Contributions to the analysis of proteins |
| title_full_unstemmed | Contributions to the analysis of proteins |
| title_short | Contributions to the analysis of proteins |
| title_sort | contributions to the analysis of proteins |
| topic | Mechanical Engineering. |
| url | http://hdl.handle.net/1721.1/67599 |
| work_keys_str_mv | AT sharifisedehreza contributionstotheanalysisofproteins |