PCR - Ligation Assembly Standard for BioBrick Parts

This Request for Comments (RFC) describes a novel method for the assembly of standard BioBrick parts. This assembly method for BioBrick parts is an improvement upon the conventional methods of BioBrick part assembly. This assembly method will involve the use of standard BioBrick restriction enzyme s...

Full description

Bibliographic Details
Main Authors: He, Tony PeiYuan, Campbell, Alexander, Zhou, Stefanie Yifan
Format: Technical Report
Language:English
Published: 2011
Subjects:
Online Access:http://hdl.handle.net/1721.1/67700
Description
Summary:This Request for Comments (RFC) describes a novel method for the assembly of standard BioBrick parts. This assembly method for BioBrick parts is an improvement upon the conventional methods of BioBrick part assembly. This assembly method will involve the use of standard BioBrick restriction enzyme systems, PCR purification kit, ligase system, and a PCR polymerase system.