The role of mir-290-295 in murine embryonic and germ cell development
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, September 2011.
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| Format: | Thesis |
| Language: | eng |
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Massachusetts Institute of Technology
2012
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| Online Access: | http://hdl.handle.net/1721.1/68429 |
| _version_ | 1826202221884211200 |
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| author | Medeiros, Lea A. (Lea Ann) |
| author2 | Rudolf Jaenisch. |
| author_facet | Rudolf Jaenisch. Medeiros, Lea A. (Lea Ann) |
| author_sort | Medeiros, Lea A. (Lea Ann) |
| collection | MIT |
| description | Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, September 2011. |
| first_indexed | 2024-09-23T12:04:06Z |
| format | Thesis |
| id | mit-1721.1/68429 |
| institution | Massachusetts Institute of Technology |
| language | eng |
| last_indexed | 2024-09-23T12:04:06Z |
| publishDate | 2012 |
| publisher | Massachusetts Institute of Technology |
| record_format | dspace |
| spelling | mit-1721.1/684292019-04-11T01:00:05Z The role of mir-290-295 in murine embryonic and germ cell development Medeiros, Lea A. (Lea Ann) Rudolf Jaenisch. Massachusetts Institute of Technology. Dept. of Biology. Massachusetts Institute of Technology. Dept. of Biology. Biology. Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, September 2011. Cataloged from PDF version of thesis. "September 2011." Includes bibliographical references. MicroRNAs, 22nt long RNAs derived from hairpin transcripts, are important regulators of gene expression and have been shown to participate in regulation of every biological process known to date. Mir-290 through mir-295 (mir-290-295) is a mammalian-specific miRNA cluster that, in the mouse, is expressed specifically in early embryos and embryonic germ cells. This thesis examines the in vivo consequences of targeted deletion of mir-290-295 in the developing mouse embryo. Mir-290-295 deficiency results in a partially penetrant embryonic lethality. Mir-290-295 is not required for early preimplantation development but is instead required for later postimplantation development. In surviving mir-290-295 deficient embryos, female but not male fertility is compromised. This impairment in fertility arises from a defect in migrating primordial germ cells and occurs equally in male and female mutant animals. Male mir-290-295-- mice, due to the extended proliferative lifespan of their germ cells, are able to recover from this initial germ cell loss and are fertile. Female mir-290-295-/- mice are unable to recover and are sterile due to premature ovarian failure. by Lea A. Medeiros. Ph.D. 2012-01-12T19:26:56Z 2012-01-12T19:26:56Z 2011 Thesis http://hdl.handle.net/1721.1/68429 768827284 eng M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 141 p. application/pdf Massachusetts Institute of Technology |
| spellingShingle | Biology. Medeiros, Lea A. (Lea Ann) The role of mir-290-295 in murine embryonic and germ cell development |
| title | The role of mir-290-295 in murine embryonic and germ cell development |
| title_full | The role of mir-290-295 in murine embryonic and germ cell development |
| title_fullStr | The role of mir-290-295 in murine embryonic and germ cell development |
| title_full_unstemmed | The role of mir-290-295 in murine embryonic and germ cell development |
| title_short | The role of mir-290-295 in murine embryonic and germ cell development |
| title_sort | role of mir 290 295 in murine embryonic and germ cell development |
| topic | Biology. |
| url | http://hdl.handle.net/1721.1/68429 |
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