Designer Lipid-Like Peptides: A Class of Detergents for Studying Functional Olfactory Receptors Using Commercial Cell-Free Systems
A crucial bottleneck in membrane protein studies, particularly G-protein coupled receptors, is the notorious difficulty of finding an optimal detergent that can solubilize them and maintain their stability and function. Here we report rapid production of 12 unique mammalian olfactory receptors using...
| Main Authors: | , , , , , , , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
Public Library of Science
2012
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| Online Access: | http://hdl.handle.net/1721.1/69044 |
| _version_ | 1826205871016771584 |
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| author | Baaske, Philipp Wienken, Christoph J. Jerabek-Willemsen, Moran Duhr, Stefan Luo, Yuan Braun, Dieter Corin, Karolina A. Ravel, Deepali B. Song, Junyao Brown, Emily E. Wang, Xiaoqiang Zhang, Shuguang |
| author2 | Massachusetts Institute of Technology. Center for Biomedical Engineering |
| author_facet | Massachusetts Institute of Technology. Center for Biomedical Engineering Baaske, Philipp Wienken, Christoph J. Jerabek-Willemsen, Moran Duhr, Stefan Luo, Yuan Braun, Dieter Corin, Karolina A. Ravel, Deepali B. Song, Junyao Brown, Emily E. Wang, Xiaoqiang Zhang, Shuguang |
| author_sort | Baaske, Philipp |
| collection | MIT |
| description | A crucial bottleneck in membrane protein studies, particularly G-protein coupled receptors, is the notorious difficulty of finding an optimal detergent that can solubilize them and maintain their stability and function. Here we report rapid production of 12 unique mammalian olfactory receptors using short designer lipid-like peptides as detergents. The peptides were able to solubilize and stabilize each receptor. Circular dichroism showed that the purified olfactory receptors had alpha-helical secondary structures. Microscale thermophoresis suggested that the receptors were functional and bound their odorants. Blot intensity measurements indicated that milligram quantities of each olfactory receptor could be produced with at least one peptide detergent. The peptide detergents' capability was comparable to that of the detergent Brij-35. The ability of 10 peptide detergents to functionally solubilize 12 olfactory receptors demonstrates their usefulness as a new class of detergents for olfactory receptors, and possibly other G-protein coupled receptors and membrane proteins. |
| first_indexed | 2024-09-23T13:20:25Z |
| format | Article |
| id | mit-1721.1/69044 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T13:20:25Z |
| publishDate | 2012 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | mit-1721.1/690442022-09-28T13:31:09Z Designer Lipid-Like Peptides: A Class of Detergents for Studying Functional Olfactory Receptors Using Commercial Cell-Free Systems Baaske, Philipp Wienken, Christoph J. Jerabek-Willemsen, Moran Duhr, Stefan Luo, Yuan Braun, Dieter Corin, Karolina A. Ravel, Deepali B. Song, Junyao Brown, Emily E. Wang, Xiaoqiang Zhang, Shuguang Massachusetts Institute of Technology. Center for Biomedical Engineering Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Biology Zhang, Shuguang Corin, Karolina A. Ravel, Deepali B. Song, Junyao Brown, Emily E. Wang, Xiaoqiang Zhang, Shuguang A crucial bottleneck in membrane protein studies, particularly G-protein coupled receptors, is the notorious difficulty of finding an optimal detergent that can solubilize them and maintain their stability and function. Here we report rapid production of 12 unique mammalian olfactory receptors using short designer lipid-like peptides as detergents. The peptides were able to solubilize and stabilize each receptor. Circular dichroism showed that the purified olfactory receptors had alpha-helical secondary structures. Microscale thermophoresis suggested that the receptors were functional and bound their odorants. Blot intensity measurements indicated that milligram quantities of each olfactory receptor could be produced with at least one peptide detergent. The peptide detergents' capability was comparable to that of the detergent Brij-35. The ability of 10 peptide detergents to functionally solubilize 12 olfactory receptors demonstrates their usefulness as a new class of detergents for olfactory receptors, and possibly other G-protein coupled receptors and membrane proteins. United States. Defense Advanced Research Projects Agency (DARPA-HR0011-09-C-0012) Massachusetts Institute of Technology. Undergraduate Research Opportunities Program 2012-02-08T18:41:37Z 2012-02-08T18:41:37Z 2011-11 2011-04 Article http://purl.org/eprint/type/JournalArticle 1932-6203 http://hdl.handle.net/1721.1/69044 Corin, Karolina et al. “Designer Lipid-Like Peptides: A Class of Detergents for Studying Functional Olfactory Receptors Using Commercial Cell-Free Systems.” Ed. Fabrizio Gelain. PLoS ONE 6.11 (2011): e25067. Web. 8 Feb. 2012. en_US http://dx.doi.org/10.1371/journal.pone.0025067 PLoS ONE Creative Commons Attribution http://creativecommons.org/licenses/by/2.5/ application/pdf Public Library of Science PLoS |
| spellingShingle | Baaske, Philipp Wienken, Christoph J. Jerabek-Willemsen, Moran Duhr, Stefan Luo, Yuan Braun, Dieter Corin, Karolina A. Ravel, Deepali B. Song, Junyao Brown, Emily E. Wang, Xiaoqiang Zhang, Shuguang Designer Lipid-Like Peptides: A Class of Detergents for Studying Functional Olfactory Receptors Using Commercial Cell-Free Systems |
| title | Designer Lipid-Like Peptides: A Class of Detergents for Studying Functional Olfactory Receptors Using Commercial Cell-Free Systems |
| title_full | Designer Lipid-Like Peptides: A Class of Detergents for Studying Functional Olfactory Receptors Using Commercial Cell-Free Systems |
| title_fullStr | Designer Lipid-Like Peptides: A Class of Detergents for Studying Functional Olfactory Receptors Using Commercial Cell-Free Systems |
| title_full_unstemmed | Designer Lipid-Like Peptides: A Class of Detergents for Studying Functional Olfactory Receptors Using Commercial Cell-Free Systems |
| title_short | Designer Lipid-Like Peptides: A Class of Detergents for Studying Functional Olfactory Receptors Using Commercial Cell-Free Systems |
| title_sort | designer lipid like peptides a class of detergents for studying functional olfactory receptors using commercial cell free systems |
| url | http://hdl.handle.net/1721.1/69044 |
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