A novel mechanism of intracellular transport: sieving by an anchored homogeneously contracting F-actin meshwork
Summary Actin-based contractility orchestrates changes in cell shape underlying cellular functions ranging from division to migration and wound healing [ [1], [2], [3], [4] and [5]]. Actin also functions in intracellular transport, with the prevailing view that filamentous actin (F-actin) cables...
| Main Authors: | , , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
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Elsevier Ltd.
2012
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| Online Access: | http://hdl.handle.net/1721.1/69075 https://orcid.org/0000-0002-6199-6855 |
| _version_ | 1826198252999933952 |
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| author | Mori, Masashi Monnier, Nilah Daigle, Nathalie Bathe, Mark Ellenberg, Jan Lenart, Peter |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Mori, Masashi Monnier, Nilah Daigle, Nathalie Bathe, Mark Ellenberg, Jan Lenart, Peter |
| author_sort | Mori, Masashi |
| collection | MIT |
| description | Summary
Actin-based contractility orchestrates changes in cell shape underlying cellular functions ranging from division to migration and wound healing [ [1], [2], [3], [4] and [5]]. Actin also functions in intracellular transport, with the prevailing view that filamentous actin (F-actin) cables serve as tracks for motor-driven transport of cargo [ [1] and [6]]. We recently discovered an alternate mode of intracellular transport in starfish oocytes involving a contractile F-actin meshwork that mediates chromosome congression [7]. The mechanisms by which this meshwork contracts and translates its contractile activity into directional transport of chromosomes remained open questions. Here, we use live-cell imaging with quantitative analysis of chromosome trajectories and meshwork velocities to show that the 3D F-actin meshwork contracts homogeneously and isotropically throughout the nuclear space. Centrifugation experiments reveal that this homogeneous contraction is translated into asymmetric, directional transport by mechanical anchoring of the meshwork to the cell cortex. Finally, by injecting inert particles of different sizes, we show that this directional transport activity is size-selective and transduced to chromosomal cargo at least in part by steric trapping or “sieving.” Taken together, these results reveal mechanistic design principles of a novel and potentially versatile mode of intracellular transport based on sieving by an anchored homogeneously contracting F-actin meshwork. |
| first_indexed | 2024-09-23T11:01:48Z |
| format | Article |
| id | mit-1721.1/69075 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T11:01:48Z |
| publishDate | 2012 |
| publisher | Elsevier Ltd. |
| record_format | dspace |
| spelling | mit-1721.1/690752022-09-27T16:39:01Z A novel mechanism of intracellular transport: sieving by an anchored homogeneously contracting F-actin meshwork Intracellular Transport by an Anchored Homogeneously Contracting F-Actin Meshwork Mori, Masashi Monnier, Nilah Daigle, Nathalie Bathe, Mark Ellenberg, Jan Lenart, Peter Massachusetts Institute of Technology. Department of Biological Engineering Bathe, Mark Bathe, Mark Monnier, Nilah Summary Actin-based contractility orchestrates changes in cell shape underlying cellular functions ranging from division to migration and wound healing [ [1], [2], [3], [4] and [5]]. Actin also functions in intracellular transport, with the prevailing view that filamentous actin (F-actin) cables serve as tracks for motor-driven transport of cargo [ [1] and [6]]. We recently discovered an alternate mode of intracellular transport in starfish oocytes involving a contractile F-actin meshwork that mediates chromosome congression [7]. The mechanisms by which this meshwork contracts and translates its contractile activity into directional transport of chromosomes remained open questions. Here, we use live-cell imaging with quantitative analysis of chromosome trajectories and meshwork velocities to show that the 3D F-actin meshwork contracts homogeneously and isotropically throughout the nuclear space. Centrifugation experiments reveal that this homogeneous contraction is translated into asymmetric, directional transport by mechanical anchoring of the meshwork to the cell cortex. Finally, by injecting inert particles of different sizes, we show that this directional transport activity is size-selective and transduced to chromosomal cargo at least in part by steric trapping or “sieving.” Taken together, these results reveal mechanistic design principles of a novel and potentially versatile mode of intracellular transport based on sieving by an anchored homogeneously contracting F-actin meshwork. 2012-02-10T16:01:01Z 2012-02-10T16:01:01Z 2011-04 2010-10 Article http://purl.org/eprint/type/JournalArticle 0960-9822 http://hdl.handle.net/1721.1/69075 Mori, Masashi et al. “Intracellular Transport by an Anchored Homogeneously Contracting F-Actin Meshwork.” Current Biology 21.7 (2011): 606-611. https://orcid.org/0000-0002-6199-6855 en_US http://dx.doi.org/10.1016/j.cub.2011.03.002 Current Biology Creative Commons Attribution-Noncommercial-Share Alike 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf Elsevier Ltd. Prof. Bathe via Howard Silver |
| spellingShingle | Mori, Masashi Monnier, Nilah Daigle, Nathalie Bathe, Mark Ellenberg, Jan Lenart, Peter A novel mechanism of intracellular transport: sieving by an anchored homogeneously contracting F-actin meshwork |
| title | A novel mechanism of intracellular transport: sieving by an anchored homogeneously contracting F-actin meshwork |
| title_full | A novel mechanism of intracellular transport: sieving by an anchored homogeneously contracting F-actin meshwork |
| title_fullStr | A novel mechanism of intracellular transport: sieving by an anchored homogeneously contracting F-actin meshwork |
| title_full_unstemmed | A novel mechanism of intracellular transport: sieving by an anchored homogeneously contracting F-actin meshwork |
| title_short | A novel mechanism of intracellular transport: sieving by an anchored homogeneously contracting F-actin meshwork |
| title_sort | novel mechanism of intracellular transport sieving by an anchored homogeneously contracting f actin meshwork |
| url | http://hdl.handle.net/1721.1/69075 https://orcid.org/0000-0002-6199-6855 |
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