A Robust and Rapid Method of Producing Soluble, Stable, and Functional G-Protein Coupled Receptors

A Robust and Rapid Method of Producing Soluble, Stable, and Functional G-Protein Coupled Receptors

Membrane proteins, particularly G-protein coupled receptors (GPCRs), are notoriously difficult to express. Using commercial E.coli cell-free systems with the detergent Brij-35, we could rapidly produce milligram quantities of 13 unique GPCRs. Immunoaffinity purification yielded receptors at >90%...

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Bibliographic Details
Main Authors: Baaske, Philipp, Geissler, Sandra, Wienken, Christoph J., Jerabek-Willemsen, Moran, Duhr, Stefan, Braun, Dieter, Corin, Karolina A., Ravel, Deepali B., Song, Junyao, Brown, Emily E., Wang, Xiaoqiang, Zhang, Shuguang
Other Authors: Massachusetts Institute of Technology. Center for Biomedical Engineering
Format: Article
Language:en_US
Published: Public Library of Science 2012
Online Access:http://hdl.handle.net/1721.1/69080

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