dlx and sp6-9 Control Optic Cup Regeneration in a Prototypic Eye
Optic cups are a structural feature of diverse eyes, from simple pit eyes to camera eyes of vertebrates and cephalopods. We used the planarian prototypic eye as a model to study the genetic control of optic cup formation and regeneration. We identified two genes encoding transcription factors, sp6-9...
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| Format: | Article |
| Language: | en_US |
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Public Library of Science
2012
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| Online Access: | http://hdl.handle.net/1721.1/69087 https://orcid.org/0000-0002-5569-333X |
| _version_ | 1826199662101528576 |
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| author | Reddien, Peter Lapan, Sylvain W. |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Reddien, Peter Lapan, Sylvain W. |
| author_sort | Reddien, Peter |
| collection | MIT |
| description | Optic cups are a structural feature of diverse eyes, from simple pit eyes to camera eyes of vertebrates and cephalopods. We used the planarian prototypic eye as a model to study the genetic control of optic cup formation and regeneration. We identified two genes encoding transcription factors, sp6-9 and dlx, that were expressed in the eye specifically in the optic cup and not the photoreceptor neurons. RNAi of these genes prevented formation of visible optic cups during regeneration. Planarian regeneration requires an adult proliferative cell population with stem cell-like properties called the neoblasts. We found that optic cup formation occurred only after migration of progressively differentiating progenitor cells from the neoblast population. The eye regeneration defect caused by dlx and sp6-9 RNAi can be explained by a failure to generate these early optic cup progenitors. Dlx and Sp6-9 genes function as a module during the development of diverse animal appendages, including vertebrate and insect limbs. Our work reveals a novel function for this gene pair in the development of a fundamental eye component, and it utilizes these genes to demonstrate a mechanism for total organ regeneration in which extensive cell movement separates new cell specification from organ morphogenesis. |
| first_indexed | 2024-09-23T11:23:30Z |
| format | Article |
| id | mit-1721.1/69087 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T11:23:30Z |
| publishDate | 2012 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | mit-1721.1/690872022-09-27T19:14:02Z dlx and sp6-9 Control Optic Cup Regeneration in a Prototypic Eye Reddien, Peter Lapan, Sylvain W. Massachusetts Institute of Technology. Department of Biology Whitehead Institute for Biomedical Research Reddien, Peter W. Lapan, Sylvain William Reddien, Peter Optic cups are a structural feature of diverse eyes, from simple pit eyes to camera eyes of vertebrates and cephalopods. We used the planarian prototypic eye as a model to study the genetic control of optic cup formation and regeneration. We identified two genes encoding transcription factors, sp6-9 and dlx, that were expressed in the eye specifically in the optic cup and not the photoreceptor neurons. RNAi of these genes prevented formation of visible optic cups during regeneration. Planarian regeneration requires an adult proliferative cell population with stem cell-like properties called the neoblasts. We found that optic cup formation occurred only after migration of progressively differentiating progenitor cells from the neoblast population. The eye regeneration defect caused by dlx and sp6-9 RNAi can be explained by a failure to generate these early optic cup progenitors. Dlx and Sp6-9 genes function as a module during the development of diverse animal appendages, including vertebrate and insect limbs. Our work reveals a novel function for this gene pair in the development of a fundamental eye component, and it utilizes these genes to demonstrate a mechanism for total organ regeneration in which extensive cell movement separates new cell specification from organ morphogenesis. United States. National Institutes of Health (NIH R01GM080639) W. M. Keck Foundation Howard Hughes Medical Institute 2012-02-10T19:21:09Z 2012-02-10T19:21:09Z 2011-08 2011-03 Article http://purl.org/eprint/type/JournalArticle 1553-7390 1553-7404 http://hdl.handle.net/1721.1/69087 Lapan, Sylvain W., and Peter W. Reddien. “Dlx and Sp6-9 Control Optic Cup Regeneration in a Prototypic Eye.” Ed. Claude Desplan. PLoS Genetics 7.8 (2011): e1002226. Web. 10 Feb. 2012. https://orcid.org/0000-0002-5569-333X en_US http://dx.doi.org/10.1371/journal.pgen.1002226 PLoS Genetics Creative Commons Attribution http://creativecommons.org/licenses/by/2.5/ application/pdf Public Library of Science PLoS |
| spellingShingle | Reddien, Peter Lapan, Sylvain W. dlx and sp6-9 Control Optic Cup Regeneration in a Prototypic Eye |
| title | dlx and sp6-9 Control Optic Cup Regeneration in a Prototypic Eye |
| title_full | dlx and sp6-9 Control Optic Cup Regeneration in a Prototypic Eye |
| title_fullStr | dlx and sp6-9 Control Optic Cup Regeneration in a Prototypic Eye |
| title_full_unstemmed | dlx and sp6-9 Control Optic Cup Regeneration in a Prototypic Eye |
| title_short | dlx and sp6-9 Control Optic Cup Regeneration in a Prototypic Eye |
| title_sort | dlx and sp6 9 control optic cup regeneration in a prototypic eye |
| url | http://hdl.handle.net/1721.1/69087 https://orcid.org/0000-0002-5569-333X |
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