Hot embossing for fabrication of a microfluidic 3D cell culture
Clinically relevant studies of cell function in vitro require a physiologically-representative microenvironment possessing aspects such as a 3D extracellular matrix (ECM) and controlled biochemical and biophysical parameters. A polydimethylsiloxane (PDMS) microfluidic system with a 3D collagen gel h...
| Автори: | , , , |
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| Інші автори: | |
| Формат: | Стаття |
| Мова: | en_US |
| Опубліковано: |
Springer Netherlands
2012
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| Онлайн доступ: | http://hdl.handle.net/1721.1/69104 https://orcid.org/0000-0002-7232-304X |
| _version_ | 1826210749308993536 |
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| author | Jeon, Jessie S. Chung, Seok Kamm, Roger Dale Charest, Joseph L. |
| author2 | Massachusetts Institute of Technology. Department of Mechanical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Mechanical Engineering Jeon, Jessie S. Chung, Seok Kamm, Roger Dale Charest, Joseph L. |
| author_sort | Jeon, Jessie S. |
| collection | MIT |
| description | Clinically relevant studies of cell function in vitro require a physiologically-representative microenvironment possessing aspects such as a 3D extracellular matrix (ECM) and controlled biochemical and biophysical parameters. A polydimethylsiloxane (PDMS) microfluidic system with a 3D collagen gel has previously served for analysis of factors inducing different responses of cells in a 3D microenvironment under controlled biochemical and biophysical parameters. In the present study, applying the known commercially-viable manufacturing methods to a cyclic olefin copolymer (COC) material resulted in a microfluidic device with enhanced 3D gel capabilities, controlled surface properties, and improved potential to serve high-volume applications. Hot embossing and roller lamination molded and sealed the microfluidic device. A combination of oxygen plasma and thermal treatments enhanced the sealing, ensured proper placement of the 3D gel, and created controlled and stable surface properties within the device. Culture of cells in the new device indicated no adverse effects of the COC material or processing as compared to previous PDMS devices. The results demonstrate a methodology to transition microfludic devices for 3D cell culture from scientific research to high-volume applications with broad clinical impact. |
| first_indexed | 2024-09-23T14:55:06Z |
| format | Article |
| id | mit-1721.1/69104 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T14:55:06Z |
| publishDate | 2012 |
| publisher | Springer Netherlands |
| record_format | dspace |
| spelling | mit-1721.1/691042022-09-29T11:23:09Z Hot embossing for fabrication of a microfluidic 3D cell culture Jeon, Jessie S. Chung, Seok Kamm, Roger Dale Charest, Joseph L. Massachusetts Institute of Technology. Department of Mechanical Engineering Kamm, Roger Dale Jeon, Jessie S. Kamm, Roger Dale Clinically relevant studies of cell function in vitro require a physiologically-representative microenvironment possessing aspects such as a 3D extracellular matrix (ECM) and controlled biochemical and biophysical parameters. A polydimethylsiloxane (PDMS) microfluidic system with a 3D collagen gel has previously served for analysis of factors inducing different responses of cells in a 3D microenvironment under controlled biochemical and biophysical parameters. In the present study, applying the known commercially-viable manufacturing methods to a cyclic olefin copolymer (COC) material resulted in a microfluidic device with enhanced 3D gel capabilities, controlled surface properties, and improved potential to serve high-volume applications. Hot embossing and roller lamination molded and sealed the microfluidic device. A combination of oxygen plasma and thermal treatments enhanced the sealing, ensured proper placement of the 3D gel, and created controlled and stable surface properties within the device. Culture of cells in the new device indicated no adverse effects of the COC material or processing as compared to previous PDMS devices. The results demonstrate a methodology to transition microfludic devices for 3D cell culture from scientific research to high-volume applications with broad clinical impact. National Cancer Institute (U.S.) (award R21CA140096) Charles Stark Draper Laboratory (IR&D Grant) 2012-02-15T13:53:11Z 2012-02-15T13:53:11Z 2010-11 Article http://purl.org/eprint/type/JournalArticle 1387-2176 1572-8781 http://hdl.handle.net/1721.1/69104 Jeon, Jessie S. et al. “Hot embossing for fabrication of a microfluidic 3D cell culture platform.” Biomedical Microdevices 13.2 (2010): 325-333. https://orcid.org/0000-0002-7232-304X en_US http://dx.doi.org/10.1007/s10544-010-9496-0 Biomedical Microdevices Creative Commons Attribution-Noncommercial-Share Alike 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf Springer Netherlands Prof. Kamm via Angie Locknar |
| spellingShingle | Jeon, Jessie S. Chung, Seok Kamm, Roger Dale Charest, Joseph L. Hot embossing for fabrication of a microfluidic 3D cell culture |
| title | Hot embossing for fabrication of a microfluidic 3D cell culture |
| title_full | Hot embossing for fabrication of a microfluidic 3D cell culture |
| title_fullStr | Hot embossing for fabrication of a microfluidic 3D cell culture |
| title_full_unstemmed | Hot embossing for fabrication of a microfluidic 3D cell culture |
| title_short | Hot embossing for fabrication of a microfluidic 3D cell culture |
| title_sort | hot embossing for fabrication of a microfluidic 3d cell culture |
| url | http://hdl.handle.net/1721.1/69104 https://orcid.org/0000-0002-7232-304X |
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