Activation of mGluR5 Induces Rapid and Long-Lasting Protein Kinase D Phosphorylation in Hippocampal Neurons
Metabotropic glutamate receptors (mGluRs), including mGluR5, play a central role in regulating the strength and plasticity of synaptic connections in the brain. However, the signaling pathways that connect mGluRs to their downstream effectors are not yet fully understood. Here, we report that stimul...
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| 语言: | en_US |
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Springer Science + Business Media
2012
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| 在线阅读: | http://hdl.handle.net/1721.1/69224 https://orcid.org/0000-0003-0582-2284 |
| _version_ | 1826192632875843584 |
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| author | Krueger, Dilja Osterweil, Emily Bear, Mark |
| author2 | Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences |
| author_facet | Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Krueger, Dilja Osterweil, Emily Bear, Mark |
| author_sort | Krueger, Dilja |
| collection | MIT |
| description | Metabotropic glutamate receptors (mGluRs), including mGluR5, play a central role in regulating the strength and plasticity of synaptic connections in the brain. However, the signaling pathways that connect mGluRs to their downstream effectors are not yet fully understood. Here, we report that stimulation of mGluR5 in hippocampal cultures and slices results in phosphorylation of protein kinase D (PKD) at the autophosphorylation site Ser-916. This phosphorylation event occurs within 30 s of stimulation, persists for at least 24 h, and is dependent on activation of phospholipase C and protein kinase C. Our data suggest that activation of PKD may represent a novel signaling pathway linking mGluR5 to its downstream targets. These findings have important implications for the study of the molecular mechanisms underlying mGluR-dependent synaptic plasticity. |
| first_indexed | 2024-09-23T09:25:04Z |
| format | Article |
| id | mit-1721.1/69224 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T09:25:04Z |
| publishDate | 2012 |
| publisher | Springer Science + Business Media |
| record_format | dspace |
| spelling | mit-1721.1/692242022-09-26T11:24:19Z Activation of mGluR5 Induces Rapid and Long-Lasting Protein Kinase D Phosphorylation in Hippocampal Neurons Krueger, Dilja Osterweil, Emily Bear, Mark Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Picower Institute for Learning and Memory Bear, Mark Bear, Mark Krueger, Dilja Osterweil, Emily Metabotropic glutamate receptors (mGluRs), including mGluR5, play a central role in regulating the strength and plasticity of synaptic connections in the brain. However, the signaling pathways that connect mGluRs to their downstream effectors are not yet fully understood. Here, we report that stimulation of mGluR5 in hippocampal cultures and slices results in phosphorylation of protein kinase D (PKD) at the autophosphorylation site Ser-916. This phosphorylation event occurs within 30 s of stimulation, persists for at least 24 h, and is dependent on activation of phospholipase C and protein kinase C. Our data suggest that activation of PKD may represent a novel signaling pathway linking mGluR5 to its downstream targets. These findings have important implications for the study of the molecular mechanisms underlying mGluR-dependent synaptic plasticity. Howard Hughes Medical Institute FRAXA Research Foundation National Institute of Mental Health (U.S.) Eunice Kennedy Shriver National Institute of Child Health and Human Development (U.S.) 2012-02-28T16:03:05Z 2012-02-28T16:03:05Z 2010-09 2010-01 Article http://purl.org/eprint/type/JournalArticle 0895-8696 1559-1166 http://hdl.handle.net/1721.1/69224 Krueger, Dilja D., Emily K. Osterweil, and Mark F. Bear. “Activation of mGluR5 Induces Rapid and Long-Lasting Protein Kinase D Phosphorylation in Hippocampal Neurons.” Journal of Molecular Neuroscience 42.1 (2010): 1–8. https://orcid.org/0000-0003-0582-2284 en_US http://dx.doi.org/10.1007/s12031-010-9338-9 Journal of Molecular Neuroscience Creative Commons Attribution-Noncommercial-Share Alike 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf Springer Science + Business Media PubMed Central |
| spellingShingle | Krueger, Dilja Osterweil, Emily Bear, Mark Activation of mGluR5 Induces Rapid and Long-Lasting Protein Kinase D Phosphorylation in Hippocampal Neurons |
| title | Activation of mGluR5 Induces Rapid and Long-Lasting Protein Kinase D Phosphorylation in Hippocampal Neurons |
| title_full | Activation of mGluR5 Induces Rapid and Long-Lasting Protein Kinase D Phosphorylation in Hippocampal Neurons |
| title_fullStr | Activation of mGluR5 Induces Rapid and Long-Lasting Protein Kinase D Phosphorylation in Hippocampal Neurons |
| title_full_unstemmed | Activation of mGluR5 Induces Rapid and Long-Lasting Protein Kinase D Phosphorylation in Hippocampal Neurons |
| title_short | Activation of mGluR5 Induces Rapid and Long-Lasting Protein Kinase D Phosphorylation in Hippocampal Neurons |
| title_sort | activation of mglur5 induces rapid and long lasting protein kinase d phosphorylation in hippocampal neurons |
| url | http://hdl.handle.net/1721.1/69224 https://orcid.org/0000-0003-0582-2284 |
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