Fabrication and Characterization of an Integrated Microsystem for Protein Preconcentration and Sensing
We report on a fabrication and packaging process for a microsystem consisting of a mass-based protein detector and a fully integrated preconcentrator. Preconcentration of protein is achieved by means of a nanofluidic concentrator (NC), which takes advantage of fast nonlinear electroosmotic flow near...
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| Format: | Article |
| Language: | en_US |
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Institute of Electrical and Electronics Engineers
2012
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| Online Access: | http://hdl.handle.net/1721.1/69557 https://orcid.org/0000-0001-5223-9433 https://orcid.org/0000-0001-7215-1439 |
| _version_ | 1826205407723388928 |
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| author | Dextras, Philip Payer, Kristofor Robert Burg, Thomas P. Shen, Wenjiang Wang, Ying-Chih Han, Jongyoon Manalis, Scott R. |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Dextras, Philip Payer, Kristofor Robert Burg, Thomas P. Shen, Wenjiang Wang, Ying-Chih Han, Jongyoon Manalis, Scott R. |
| author_sort | Dextras, Philip |
| collection | MIT |
| description | We report on a fabrication and packaging process for a microsystem consisting of a mass-based protein detector and a fully integrated preconcentrator. Preconcentration of protein is achieved by means of a nanofluidic concentrator (NC), which takes advantage of fast nonlinear electroosmotic flow near a nanochannel-microchannel junction to concentrate charged molecules inside a volume of fluid on the order of 1 pL. Detection of preconcentrated protein samples is accomplished by passing them through a suspended microchannel resonator (SMR), which is a hollow resonant cantilever serially connected to the NC on the same device. The transit of a preconcentrated sample produces a transient shift in the cantilever's resonance frequency that is proportional to the density of the sample and, hence, the concentration of protein contained in it. A device containing both NC and SMR structures was produced using a novel fabrication process which simultaneously satisfies the separate packaging requirements of the two structures. The initial testing of this prototype device has demonstrated that the integrated SMR can accurately measure the concentration of a bovine serum albumin solution, that was preconcentrated using the integrated NC. Future improvements in the fabrication process will allow site-specific surface modification of the device and compatibility with separation methods, which will create opportunities for its application to immunoassays and universal detection. |
| first_indexed | 2024-09-23T13:12:12Z |
| format | Article |
| id | mit-1721.1/69557 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T13:12:12Z |
| publishDate | 2012 |
| publisher | Institute of Electrical and Electronics Engineers |
| record_format | dspace |
| spelling | mit-1721.1/695572022-10-01T13:44:57Z Fabrication and Characterization of an Integrated Microsystem for Protein Preconcentration and Sensing Dextras, Philip Payer, Kristofor Robert Burg, Thomas P. Shen, Wenjiang Wang, Ying-Chih Han, Jongyoon Manalis, Scott R. Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science Massachusetts Institute of Technology. Department of Mechanical Engineering Massachusetts Institute of Technology. Microsystems Technology Laboratories Manalis, Scott R. Manalis, Scott R. Dextras, Philip Payer, Kristofor Robert Burg, Thomas P. Wang, Ying-Chih Han, Jongyoon We report on a fabrication and packaging process for a microsystem consisting of a mass-based protein detector and a fully integrated preconcentrator. Preconcentration of protein is achieved by means of a nanofluidic concentrator (NC), which takes advantage of fast nonlinear electroosmotic flow near a nanochannel-microchannel junction to concentrate charged molecules inside a volume of fluid on the order of 1 pL. Detection of preconcentrated protein samples is accomplished by passing them through a suspended microchannel resonator (SMR), which is a hollow resonant cantilever serially connected to the NC on the same device. The transit of a preconcentrated sample produces a transient shift in the cantilever's resonance frequency that is proportional to the density of the sample and, hence, the concentration of protein contained in it. A device containing both NC and SMR structures was produced using a novel fabrication process which simultaneously satisfies the separate packaging requirements of the two structures. The initial testing of this prototype device has demonstrated that the integrated SMR can accurately measure the concentration of a bovine serum albumin solution, that was preconcentrated using the integrated NC. Future improvements in the fabrication process will allow site-specific surface modification of the device and compatibility with separation methods, which will create opportunities for its application to immunoassays and universal detection. National Cancer Institute (U.S.) (Contract R01CA119402) National Institutes of Health (U.S.) (NIH Cell Decision Process Center P50-GM68762) 2012-03-01T21:27:50Z 2012-03-01T21:27:50Z 2011-02 2010-09 Article http://purl.org/eprint/type/JournalArticle 1057-7157 INSPEC Accession Number: 11804841 http://hdl.handle.net/1721.1/69557 Dextras, Philip et al. “Fabrication and Characterization of an Integrated Microsystem for Protein Preconcentration and Sensing.” Journal of Microelectromechanical Systems 20.1 (2011): 221–230. https://orcid.org/0000-0001-5223-9433 https://orcid.org/0000-0001-7215-1439 en_US http://dx.doi.org/10.1109/JMEMS.2010.2093563 Journal of Microelectromechanical Systems Creative Commons Attribution-Noncommercial-Share Alike 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf Institute of Electrical and Electronics Engineers Prof. Manalis via Howard Silver |
| spellingShingle | Dextras, Philip Payer, Kristofor Robert Burg, Thomas P. Shen, Wenjiang Wang, Ying-Chih Han, Jongyoon Manalis, Scott R. Fabrication and Characterization of an Integrated Microsystem for Protein Preconcentration and Sensing |
| title | Fabrication and Characterization of an Integrated Microsystem for Protein Preconcentration and Sensing |
| title_full | Fabrication and Characterization of an Integrated Microsystem for Protein Preconcentration and Sensing |
| title_fullStr | Fabrication and Characterization of an Integrated Microsystem for Protein Preconcentration and Sensing |
| title_full_unstemmed | Fabrication and Characterization of an Integrated Microsystem for Protein Preconcentration and Sensing |
| title_short | Fabrication and Characterization of an Integrated Microsystem for Protein Preconcentration and Sensing |
| title_sort | fabrication and characterization of an integrated microsystem for protein preconcentration and sensing |
| url | http://hdl.handle.net/1721.1/69557 https://orcid.org/0000-0001-5223-9433 https://orcid.org/0000-0001-7215-1439 |
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