Monitoring protein interactions and dynamics with solvatochromic fluorophores
Solvatochromic fluorophores possess emission properties that are sensitive to the nature of the local microenvironment. These dyes have been exploited in applications ranging from the study of protein structural dynamics to the detection of protein-binding interactions. Although the solvatochromic i...
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| Format: | Article |
| Language: | en_US |
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Elsevier B.V.
2012
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| Online Access: | http://hdl.handle.net/1721.1/69814 https://orcid.org/0000-0002-5749-7869 |
| _version_ | 1826205509279023104 |
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| author | Loving, Galen S. Sainlos, Matthieu Imperiali, Barbara |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Loving, Galen S. Sainlos, Matthieu Imperiali, Barbara |
| author_sort | Loving, Galen S. |
| collection | MIT |
| description | Solvatochromic fluorophores possess emission properties that are sensitive to the nature of the local microenvironment. These dyes have been exploited in applications ranging from the study of protein structural dynamics to the detection of protein-binding interactions. Although the solvatochromic indole fluorophore of tryptophan has been utilized extensively for in vitro studies to advance our understanding of basic protein biochemistry, the emergence of new extrinsic synthetic dyes with improved properties, in conjunction with recent developments in site-selective methods to incorporate these chemical tools into proteins, now open the way for studies in more complex systems. Herein, we discuss recent technological advancements and their application in the design of powerful reporters, which serve critical roles in modern cell biology and assay development. |
| first_indexed | 2024-09-23T13:14:12Z |
| format | Article |
| id | mit-1721.1/69814 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T13:14:12Z |
| publishDate | 2012 |
| publisher | Elsevier B.V. |
| record_format | dspace |
| spelling | mit-1721.1/698142022-09-28T12:49:38Z Monitoring protein interactions and dynamics with solvatochromic fluorophores Loving, Galen S. Sainlos, Matthieu Imperiali, Barbara Massachusetts Institute of Technology. Department of Biology Massachusetts Institute of Technology. Department of Chemistry Imperiali, Barbara Imperiali, Barbara Sainlos, Matthieu Loving, Galen S. Solvatochromic fluorophores possess emission properties that are sensitive to the nature of the local microenvironment. These dyes have been exploited in applications ranging from the study of protein structural dynamics to the detection of protein-binding interactions. Although the solvatochromic indole fluorophore of tryptophan has been utilized extensively for in vitro studies to advance our understanding of basic protein biochemistry, the emergence of new extrinsic synthetic dyes with improved properties, in conjunction with recent developments in site-selective methods to incorporate these chemical tools into proteins, now open the way for studies in more complex systems. Herein, we discuss recent technological advancements and their application in the design of powerful reporters, which serve critical roles in modern cell biology and assay development. National Institutes of Health (U.S.) (NIH Cell Migration Consortium (GM064346)) National Science Foundation (U.S.) (CHE-0414243) National Institutes of Health (U.S.) (Interdepartmental Biotechnology Training Grant T32 GM08334) European Commission (Marie Curie Postdoctoral Fellowship Program) 2012-03-16T16:36:23Z 2012-03-16T16:36:23Z 2010-02 Article http://purl.org/eprint/type/JournalArticle 0167-7799 http://hdl.handle.net/1721.1/69814 Loving, Galen S., Matthieu Sainlos, and Barbara Imperiali. “Monitoring Protein Interactions and Dynamics with Solvatochromic Fluorophores.” Trends in Biotechnology 28.2 (2010): 73–83. https://orcid.org/0000-0002-5749-7869 en_US http://dx.doi.org/10.1016/j.tibtech.2009.11.002 Trends in Biotechnology Creative Commons Attribution-Noncommercial-Share Alike 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf Elsevier B.V. Prof. Imperiali via Erja Kajosalo |
| spellingShingle | Loving, Galen S. Sainlos, Matthieu Imperiali, Barbara Monitoring protein interactions and dynamics with solvatochromic fluorophores |
| title | Monitoring protein interactions and dynamics with solvatochromic fluorophores |
| title_full | Monitoring protein interactions and dynamics with solvatochromic fluorophores |
| title_fullStr | Monitoring protein interactions and dynamics with solvatochromic fluorophores |
| title_full_unstemmed | Monitoring protein interactions and dynamics with solvatochromic fluorophores |
| title_short | Monitoring protein interactions and dynamics with solvatochromic fluorophores |
| title_sort | monitoring protein interactions and dynamics with solvatochromic fluorophores |
| url | http://hdl.handle.net/1721.1/69814 https://orcid.org/0000-0002-5749-7869 |
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