Development and quantitative analyses of a universal rRNA-subtraction protocol for microbial metatranscriptomics
Metatranscriptomes generated by pyrosequencing hold significant potential for describing functional processes in complex microbial communities. Meeting this potential requires protocols that maximize mRNA recovery by reducing the relative abundance of ribosomal RNA, as well as systematic comparisons...
Main Authors: | Stewart, Frank J., Ottesen, Elizabeth, DeLong, Edward |
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Other Authors: | Massachusetts Institute of Technology. Department of Civil and Environmental Engineering |
Format: | Article |
Language: | en_US |
Published: |
Nature Publishing Group
2012
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Online Access: | http://hdl.handle.net/1721.1/69861 |
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