Integrated single-cell analysis shows Pichia pastoris secretes protein stochastically.
The production of heterologous proteins by secretion from cellular hosts is an important determinant for the cost of biotherapeutics. A single-cell analytical method called microengraving was used to examine the heterogeneity in secretion by the methylotrophic yeast Pichia pastoris. We show that con...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
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John Wiley & Sons, Inc.
2012
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| Online Access: | http://hdl.handle.net/1721.1/69920 https://orcid.org/0000-0003-0921-3144 |
| _version_ | 1826217717696299008 |
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| author | Love, Kerry Routenberg Panagiotou, Vasiliki Jiang, Bo Stadheim, Terrance A. Love, J. Christopher |
| author2 | Massachusetts Institute of Technology. Department of Chemical Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Chemical Engineering Love, Kerry Routenberg Panagiotou, Vasiliki Jiang, Bo Stadheim, Terrance A. Love, J. Christopher |
| author_sort | Love, Kerry Routenberg |
| collection | MIT |
| description | The production of heterologous proteins by secretion from cellular hosts is an important determinant for the cost of biotherapeutics. A single-cell analytical method called microengraving was used to examine the heterogeneity in secretion by the methylotrophic yeast Pichia pastoris. We show that constitutive secretion of a human Fc fragment by P. pastoris is not cell-cycle dependent, but rather fluctuates between states of high and low productivity in a stochastic manner. |
| first_indexed | 2024-09-23T17:08:04Z |
| format | Article |
| id | mit-1721.1/69920 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T17:08:04Z |
| publishDate | 2012 |
| publisher | John Wiley & Sons, Inc. |
| record_format | dspace |
| spelling | mit-1721.1/699202022-09-29T23:52:48Z Integrated single-cell analysis shows Pichia pastoris secretes protein stochastically. Love, Kerry Routenberg Panagiotou, Vasiliki Jiang, Bo Stadheim, Terrance A. Love, J. Christopher Massachusetts Institute of Technology. Department of Chemical Engineering Love, J. Christopher Love, J. Christopher Panagiotou, Vasiliki Love, Kerry Routenberg The production of heterologous proteins by secretion from cellular hosts is an important determinant for the cost of biotherapeutics. A single-cell analytical method called microengraving was used to examine the heterogeneity in secretion by the methylotrophic yeast Pichia pastoris. We show that constitutive secretion of a human Fc fragment by P. pastoris is not cell-cycle dependent, but rather fluctuates between states of high and low productivity in a stochastic manner. Merck Research Laboratories 2012-04-04T14:56:52Z 2012-04-04T14:56:52Z 2010-06 2010-12 Article http://purl.org/eprint/type/JournalArticle 0006-3592 1097-0290 http://hdl.handle.net/1721.1/69920 Love, Kerry Routenberg et al. “Integrated Single-cell Analysis Shows Pichia Pastoris Secretes Protein Stochastically.” Biotechnology and Bioengineering, 106.2 (2010): 319–325. https://orcid.org/0000-0003-0921-3144 en_US http://dx.doi.org/10.1002/bit.22688 Biotechnology and Bioengineering Creative Commons Attribution-Noncommercial-Share Alike 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf John Wiley & Sons, Inc. Prof. Love via Angie Locknar |
| spellingShingle | Love, Kerry Routenberg Panagiotou, Vasiliki Jiang, Bo Stadheim, Terrance A. Love, J. Christopher Integrated single-cell analysis shows Pichia pastoris secretes protein stochastically. |
| title | Integrated single-cell analysis shows Pichia pastoris secretes protein stochastically. |
| title_full | Integrated single-cell analysis shows Pichia pastoris secretes protein stochastically. |
| title_fullStr | Integrated single-cell analysis shows Pichia pastoris secretes protein stochastically. |
| title_full_unstemmed | Integrated single-cell analysis shows Pichia pastoris secretes protein stochastically. |
| title_short | Integrated single-cell analysis shows Pichia pastoris secretes protein stochastically. |
| title_sort | integrated single cell analysis shows pichia pastoris secretes protein stochastically |
| url | http://hdl.handle.net/1721.1/69920 https://orcid.org/0000-0003-0921-3144 |
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