Integrated single-cell analysis shows Pichia pastoris secretes protein stochastically.
The production of heterologous proteins by secretion from cellular hosts is an important determinant for the cost of biotherapeutics. A single-cell analytical method called microengraving was used to examine the heterogeneity in secretion by the methylotrophic yeast Pichia pastoris. We show that con...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Article |
| Language: | en_US |
| Published: |
John Wiley & Sons, Inc.
2012
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| Online Access: | http://hdl.handle.net/1721.1/69920 https://orcid.org/0000-0003-0921-3144 |