Effect of Gold Nanorod Surface Chemistry on Cellular Response
Gold nanorods (GNRs) stabilized with cetyltrimethylammonium bromide (CTAB) and GNR functionalized via a ligand exchange method with either thiolated polyethylene glycol (PEG5000) or mercaptohexadecanoic acid (MHDA) were investigated for their stability in biological media and subsequent toxicologica...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | en_US |
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American Chemical Society
2012
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| Online Access: | http://hdl.handle.net/1721.1/69944 |
| _version_ | 1826190916981882880 |
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| author | Grabinski, Christin Schaeublin, Nicole Wijaya, Andy D’Couto, Helen Baxamusa, Salmaan H. Hamad-Schifferli, Kimberly Hussain, Saber M. |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Grabinski, Christin Schaeublin, Nicole Wijaya, Andy D’Couto, Helen Baxamusa, Salmaan H. Hamad-Schifferli, Kimberly Hussain, Saber M. |
| author_sort | Grabinski, Christin |
| collection | MIT |
| description | Gold nanorods (GNRs) stabilized with cetyltrimethylammonium bromide (CTAB) and GNR functionalized via a ligand exchange method with either thiolated polyethylene glycol (PEG5000) or mercaptohexadecanoic acid (MHDA) were investigated for their stability in biological media and subsequent toxicological effects to HaCaT cells. GNR-PEG and GNR-MHDA exhibited minimal effects on cell proliferation, whereas GNR-CTAB reduced cell proliferation significantly due to the inherent toxicity of the cationic surfactant to cells. Cell uptake studies indicated relatively low uptake for GNR-PEG and high uptake for GNR-MHDA. Reverse transcriptase polymerase chain reaction (RT-PCR) revealed that GNR-PEG induced less significant and unique changes in the transcription levels of 84 genes related to stress and toxicity compared to GNR-MHDA. The results demonstrate that, although cell proliferation was not affected by both particles, there is a significant difference in gene expression in GNR-MHDA exposed cells, suggesting long-term implications for chronic exposure. |
| first_indexed | 2024-09-23T08:47:17Z |
| format | Article |
| id | mit-1721.1/69944 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T08:47:17Z |
| publishDate | 2012 |
| publisher | American Chemical Society |
| record_format | dspace |
| spelling | mit-1721.1/699442022-09-23T14:31:36Z Effect of Gold Nanorod Surface Chemistry on Cellular Response Grabinski, Christin Schaeublin, Nicole Wijaya, Andy D’Couto, Helen Baxamusa, Salmaan H. Hamad-Schifferli, Kimberly Hussain, Saber M. Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Chemical Engineering Massachusetts Institute of Technology. Department of Mechanical Engineering Hamad-Schifferli, Kimberly Wijaya, Andy D'Couto, Helen Baxamusa, Salmaan H. Hamad-Schifferli, Kimberly Gold nanorods (GNRs) stabilized with cetyltrimethylammonium bromide (CTAB) and GNR functionalized via a ligand exchange method with either thiolated polyethylene glycol (PEG5000) or mercaptohexadecanoic acid (MHDA) were investigated for their stability in biological media and subsequent toxicological effects to HaCaT cells. GNR-PEG and GNR-MHDA exhibited minimal effects on cell proliferation, whereas GNR-CTAB reduced cell proliferation significantly due to the inherent toxicity of the cationic surfactant to cells. Cell uptake studies indicated relatively low uptake for GNR-PEG and high uptake for GNR-MHDA. Reverse transcriptase polymerase chain reaction (RT-PCR) revealed that GNR-PEG induced less significant and unique changes in the transcription levels of 84 genes related to stress and toxicity compared to GNR-MHDA. The results demonstrate that, although cell proliferation was not affected by both particles, there is a significant difference in gene expression in GNR-MHDA exposed cells, suggesting long-term implications for chronic exposure. Oak Ridge Institute for Science and Education National Science Foundation (U.S.) (NSF DMR # 0906838) 2012-04-05T14:07:43Z 2012-04-05T14:07:43Z 2011-04 2010-12 Article http://purl.org/eprint/type/JournalArticle 1936-0851 1936-086X http://hdl.handle.net/1721.1/69944 Grabinski, Christin et al. “Effect of Gold Nanorod Surface Chemistry on Cellular Response.” ACS Nano 5.4 (2011): 2870–2879. en_US http://dx.doi.org/10.1021/nn103476x ACS Nano Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf American Chemical Society Prof. Hamad-Schifferli |
| spellingShingle | Grabinski, Christin Schaeublin, Nicole Wijaya, Andy D’Couto, Helen Baxamusa, Salmaan H. Hamad-Schifferli, Kimberly Hussain, Saber M. Effect of Gold Nanorod Surface Chemistry on Cellular Response |
| title | Effect of Gold Nanorod Surface Chemistry on Cellular Response |
| title_full | Effect of Gold Nanorod Surface Chemistry on Cellular Response |
| title_fullStr | Effect of Gold Nanorod Surface Chemistry on Cellular Response |
| title_full_unstemmed | Effect of Gold Nanorod Surface Chemistry on Cellular Response |
| title_short | Effect of Gold Nanorod Surface Chemistry on Cellular Response |
| title_sort | effect of gold nanorod surface chemistry on cellular response |
| url | http://hdl.handle.net/1721.1/69944 |
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