Millisecond-Timescale Optical Control of Neural Dynamics in the Nonhuman Primate Brain
To understand how brain states and behaviors are generated by neural circuits, it would be useful to be able to perturb precisely the activity of specific cell types and pathways in the nonhuman primate nervous system. We used lentivirus to target the light-activated cation channel channelrhodopsin-...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | en_US |
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Elsevier B.V.
2012
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| Online Access: | http://hdl.handle.net/1721.1/70046 https://orcid.org/0000-0002-8860-5914 https://orcid.org/0000-0003-3466-8706 https://orcid.org/0000-0002-0419-3351 https://orcid.org/0000-0002-5938-4227 https://orcid.org/0000-0002-8381-7555 https://orcid.org/0000-0001-7132-8706 https://orcid.org/0000-0002-4326-7720 |
| _version_ | 1811082461502767104 |
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| author | Han, Xue Qian, Xiaofeng Bernstein, Jacob G. Zhou, Huihui Talei Franzesi, Giovanni Stern, Patrick Bronson, Roderick T. Desimone, Robert Graybiel, Ann M Boyden, Edward |
| author2 | Massachusetts Institute of Technology. Synthetic Neurobiology Group |
| author_facet | Massachusetts Institute of Technology. Synthetic Neurobiology Group Han, Xue Qian, Xiaofeng Bernstein, Jacob G. Zhou, Huihui Talei Franzesi, Giovanni Stern, Patrick Bronson, Roderick T. Desimone, Robert Graybiel, Ann M Boyden, Edward |
| author_sort | Han, Xue |
| collection | MIT |
| description | To understand how brain states and behaviors are generated by neural circuits, it would be useful to be able to perturb precisely the activity of specific cell types and pathways in the nonhuman primate nervous system. We used lentivirus to target the light-activated cation channel channelrhodopsin-2 (ChR2) specifically to excitatory neurons of the macaque frontal cortex. Using a laser-coupled optical fiber in conjunction with a recording microelectrode, we showed that activation of excitatory neurons resulted in well-timed excitatory and suppressive influences on neocortical neural networks. ChR2 was safely expressed, and could mediate optical neuromodulation, in primate neocortex over many months. These findings highlight a methodology for investigating the causal role of specific cell types in nonhuman primate neural computation, cognition, and behavior, and open up the possibility of a new generation of ultraprecise neurological and psychiatric therapeutics via cell-type-specific optical neural control prosthetics. |
| first_indexed | 2024-09-23T12:03:47Z |
| format | Article |
| id | mit-1721.1/70046 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T12:03:47Z |
| publishDate | 2012 |
| publisher | Elsevier B.V. |
| record_format | dspace |
| spelling | mit-1721.1/700462022-09-27T23:48:43Z Millisecond-Timescale Optical Control of Neural Dynamics in the Nonhuman Primate Brain Han, Xue Qian, Xiaofeng Bernstein, Jacob G. Zhou, Huihui Talei Franzesi, Giovanni Stern, Patrick Bronson, Roderick T. Desimone, Robert Graybiel, Ann M Boyden, Edward Massachusetts Institute of Technology. Synthetic Neurobiology Group Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Massachusetts Institute of Technology. Media Laboratory McGovern Institute for Brain Research at MIT Koch Institute for Integrative Cancer Research at MIT Desimone, Robert Han, Xue Qian, Xiaofeng Bernstein, Jacob G. Zhou, Huihui Talei Franzesi, Giovanni Stern, Patrick Bronson, Roderick T. Graybiel, Ann M. Desimone, Robert Boyden, Edward Stuart To understand how brain states and behaviors are generated by neural circuits, it would be useful to be able to perturb precisely the activity of specific cell types and pathways in the nonhuman primate nervous system. We used lentivirus to target the light-activated cation channel channelrhodopsin-2 (ChR2) specifically to excitatory neurons of the macaque frontal cortex. Using a laser-coupled optical fiber in conjunction with a recording microelectrode, we showed that activation of excitatory neurons resulted in well-timed excitatory and suppressive influences on neocortical neural networks. ChR2 was safely expressed, and could mediate optical neuromodulation, in primate neocortex over many months. These findings highlight a methodology for investigating the causal role of specific cell types in nonhuman primate neural computation, cognition, and behavior, and open up the possibility of a new generation of ultraprecise neurological and psychiatric therapeutics via cell-type-specific optical neural control prosthetics. Helen Hay Whitney Foundation (Fellowship) National Institutes of Health (U.S.) (NIH-EY002621-31) McGovern Institute for Brain Research at MIT (Neurotechnology Award) National Institutes of Health (U.S.) (Grant NIH-EY12848) National Institutes of Health (U.S.) (Grant NIH-EY017292) National Institutes of Health (U.S.) (NIH Director's New Innovator Award (DP2 OD002002-01)) Brain & Behavior Research Foundation United States. Dept. of Defense National Science Foundation (U.S.) Alfred P. Sloan Foundation Dr. Gerald Burnett and Marjorie Burnett SFN Research Award for Innovation in Neuroscience Massachusetts Institute of Technology. Media Laboratory Benesse Foundation Wallace H. Coulter Foundation 2012-04-18T15:13:38Z 2012-04-18T15:13:38Z 2009-04 Article http://purl.org/eprint/type/JournalArticle 0896-6273 1097-4199 http://hdl.handle.net/1721.1/70046 Han, Xue et al. “Millisecond-Timescale Optical Control of Neural Dynamics in the Nonhuman Primate Brain.” Neuron 62.2 (2009): 191–198. Web. https://orcid.org/0000-0002-8860-5914 https://orcid.org/0000-0003-3466-8706 https://orcid.org/0000-0002-0419-3351 https://orcid.org/0000-0002-5938-4227 https://orcid.org/0000-0002-8381-7555 https://orcid.org/0000-0001-7132-8706 https://orcid.org/0000-0002-4326-7720 en_US http://dx.doi.org/10.1016/j.neuron.2009.03.011 Neuron Creative Commons Attribution-Noncommercial-Share Alike 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/ application/pdf Elsevier B.V. PubMed Central |
| spellingShingle | Han, Xue Qian, Xiaofeng Bernstein, Jacob G. Zhou, Huihui Talei Franzesi, Giovanni Stern, Patrick Bronson, Roderick T. Desimone, Robert Graybiel, Ann M Boyden, Edward Millisecond-Timescale Optical Control of Neural Dynamics in the Nonhuman Primate Brain |
| title | Millisecond-Timescale Optical Control of Neural Dynamics in the Nonhuman Primate Brain |
| title_full | Millisecond-Timescale Optical Control of Neural Dynamics in the Nonhuman Primate Brain |
| title_fullStr | Millisecond-Timescale Optical Control of Neural Dynamics in the Nonhuman Primate Brain |
| title_full_unstemmed | Millisecond-Timescale Optical Control of Neural Dynamics in the Nonhuman Primate Brain |
| title_short | Millisecond-Timescale Optical Control of Neural Dynamics in the Nonhuman Primate Brain |
| title_sort | millisecond timescale optical control of neural dynamics in the nonhuman primate brain |
| url | http://hdl.handle.net/1721.1/70046 https://orcid.org/0000-0002-8860-5914 https://orcid.org/0000-0003-3466-8706 https://orcid.org/0000-0002-0419-3351 https://orcid.org/0000-0002-5938-4227 https://orcid.org/0000-0002-8381-7555 https://orcid.org/0000-0001-7132-8706 https://orcid.org/0000-0002-4326-7720 |
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