Caenorhabditis elegans NPR-1–mediated behaviors are suppressed in the presence of mucoid bacteria
Caenorhabditis elegans exhibits a diverse range of behaviors in response to bacteria. The presence of bacterial food influences C. elegans aerotaxis, aggregation, locomotion, and pathogen avoidance behaviors through the activity of the NPR-1 neuropeptide receptor. Here, we show that mucoid strains o...
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| Format: | Article |
| Language: | en_US |
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National Academy of Sciences (U.S.)
2012
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| Online Access: | http://hdl.handle.net/1721.1/70555 https://orcid.org/0000-0002-4109-5152 https://orcid.org/0000-0002-1693-4524 |
| _version_ | 1811090752264994816 |
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| author | Reddy, Kirthi C. Hunter, Ryan C. Bhatla, Nikhil Newman, Dianne K. Kim, Dennis H. |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Reddy, Kirthi C. Hunter, Ryan C. Bhatla, Nikhil Newman, Dianne K. Kim, Dennis H. |
| author_sort | Reddy, Kirthi C. |
| collection | MIT |
| description | Caenorhabditis elegans exhibits a diverse range of behaviors in response to bacteria. The presence of bacterial food influences C. elegans aerotaxis, aggregation, locomotion, and pathogen avoidance behaviors through the activity of the NPR-1 neuropeptide receptor. Here, we show that mucoid strains of bacteria that produce an exopolysaccharide matrix do not induce NPR-1–dependent behaviors. In the presence of mucoid strains of bacteria, the C. elegans laboratory wild-type (WT) strain N2 exhibits behaviors characteristic of wild isolates and mutants with reduced NPR-1 activity. Specifically, N2 exhibits lawn bordering and roaming behavior on mucoid nonpathogenic bacteria and loss of pathogen avoidance on mucoid Pseudomonas aeruginosa. Alginate biosynthesis by laboratory and clinical isolates of mucoid P. aeruginosa is necessary and sufficient to attenuate NPR-1–mediated behavior and it suppresses C. elegans pathogen avoidance behavior. Our data suggest that the specific interaction with nonmucoid bacteria induces NPR-1–dependent behaviors of C. elegans. These observations provide an example of how exopolysaccharide matrix biosynthesis by a community of bacteria may inhibit specific host responses to microbes. |
| first_indexed | 2024-09-23T14:51:24Z |
| format | Article |
| id | mit-1721.1/70555 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T14:51:24Z |
| publishDate | 2012 |
| publisher | National Academy of Sciences (U.S.) |
| record_format | dspace |
| spelling | mit-1721.1/705552022-09-29T10:59:35Z Caenorhabditis elegans NPR-1–mediated behaviors are suppressed in the presence of mucoid bacteria Reddy, Kirthi C. Hunter, Ryan C. Bhatla, Nikhil Newman, Dianne K. Kim, Dennis H. Massachusetts Institute of Technology. Department of Biology Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences Kim, Dennis H. Kim, Dennis H. Reddy, Kirthi C. Bhatla, Nikhil Caenorhabditis elegans exhibits a diverse range of behaviors in response to bacteria. The presence of bacterial food influences C. elegans aerotaxis, aggregation, locomotion, and pathogen avoidance behaviors through the activity of the NPR-1 neuropeptide receptor. Here, we show that mucoid strains of bacteria that produce an exopolysaccharide matrix do not induce NPR-1–dependent behaviors. In the presence of mucoid strains of bacteria, the C. elegans laboratory wild-type (WT) strain N2 exhibits behaviors characteristic of wild isolates and mutants with reduced NPR-1 activity. Specifically, N2 exhibits lawn bordering and roaming behavior on mucoid nonpathogenic bacteria and loss of pathogen avoidance on mucoid Pseudomonas aeruginosa. Alginate biosynthesis by laboratory and clinical isolates of mucoid P. aeruginosa is necessary and sufficient to attenuate NPR-1–mediated behavior and it suppresses C. elegans pathogen avoidance behavior. Our data suggest that the specific interaction with nonmucoid bacteria induces NPR-1–dependent behaviors of C. elegans. These observations provide an example of how exopolysaccharide matrix biosynthesis by a community of bacteria may inhibit specific host responses to microbes. National Institutes of Health (U.S.) (NIH GM084477) Burroughs Wellcome Fund (Career Award in the Biomedical Sciences) National Science Foundation (U.S.) (Graduate Research Fellowship) Massachusetts Institute of Technology (Swanson Career Development award) Jane Coffin Childs Memorial Fund for Medical Research Medical Foundation, inc. (Charles A. King Trust Postdoctoral Research Fellowship Program) Canadian Cystic Fibrosis Foundation Howard Hughes Medical Institute (Investigator) 2012-05-09T20:59:04Z 2012-05-09T20:59:04Z 2011-08 2011-05 Article http://purl.org/eprint/type/JournalArticle 0027-8424 1091-6490 http://hdl.handle.net/1721.1/70555 Reddy, K. C. et al. “Caenorhabditis Elegans NPR-1-mediated Behaviors Are Suppressed in the Presence of Mucoid Bacteria.” Proceedings of the National Academy of Sciences 108.31 (2011): 12887–12892. Web. © 2011 by the National Academy of Sciences. https://orcid.org/0000-0002-4109-5152 https://orcid.org/0000-0002-1693-4524 en_US http://dx.doi.org/10.1073/pnas.1108265108 Proceedings of the National Academy of Sciences of the United States of America Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. application/pdf National Academy of Sciences (U.S.) PNAS |
| spellingShingle | Reddy, Kirthi C. Hunter, Ryan C. Bhatla, Nikhil Newman, Dianne K. Kim, Dennis H. Caenorhabditis elegans NPR-1–mediated behaviors are suppressed in the presence of mucoid bacteria |
| title | Caenorhabditis elegans NPR-1–mediated behaviors are suppressed in the presence of mucoid bacteria |
| title_full | Caenorhabditis elegans NPR-1–mediated behaviors are suppressed in the presence of mucoid bacteria |
| title_fullStr | Caenorhabditis elegans NPR-1–mediated behaviors are suppressed in the presence of mucoid bacteria |
| title_full_unstemmed | Caenorhabditis elegans NPR-1–mediated behaviors are suppressed in the presence of mucoid bacteria |
| title_short | Caenorhabditis elegans NPR-1–mediated behaviors are suppressed in the presence of mucoid bacteria |
| title_sort | caenorhabditis elegans npr 1 mediated behaviors are suppressed in the presence of mucoid bacteria |
| url | http://hdl.handle.net/1721.1/70555 https://orcid.org/0000-0002-4109-5152 https://orcid.org/0000-0002-1693-4524 |
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