Enteric Infection with Citrobacter rodentium Induces Coagulative Liver Necrosis and Hepatic Inflammation Prior to Peak Infection and Colonic Disease
Acute and chronic forms of inflammation are known to affect liver responses and susceptibility to disease and injury. Furthermore, intestinal microbiota has been shown critical in mediating inflammatory host responses in various animal models. Using C. rodentium, a known enteric bacterial pathogen,...
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| Format: | Article |
| Language: | en_US |
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Public Library of Science
2012
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| Online Access: | http://hdl.handle.net/1721.1/70940 https://orcid.org/0000-0001-9307-6116 |
| _version_ | 1826205106248351744 |
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| author | Raczynski, Arkadiusz R. Muthupalani, Sureshkumar Schlieper, Katherine Ann Fox, James G. Tannenbaum, Steven Robert Schauer, David B. |
| author2 | Massachusetts Institute of Technology. Department of Biological Engineering |
| author_facet | Massachusetts Institute of Technology. Department of Biological Engineering Raczynski, Arkadiusz R. Muthupalani, Sureshkumar Schlieper, Katherine Ann Fox, James G. Tannenbaum, Steven Robert Schauer, David B. |
| author_sort | Raczynski, Arkadiusz R. |
| collection | MIT |
| description | Acute and chronic forms of inflammation are known to affect liver responses and susceptibility to disease and injury. Furthermore, intestinal microbiota has been shown critical in mediating inflammatory host responses in various animal models. Using C. rodentium, a known enteric bacterial pathogen, we examined liver responses to gastrointestinal infection at various stages of disease pathogenesis. For the first time, to our knowledge, we show distinct liver pathology associated with enteric infection with C. rodentium in C57BL/6 mice, characterized by increased inflammation and hepatitis index scores as well as prominent periportal hepatocellular coagulative necrosis indicative of thrombotic ischemic injury in a subset of animals during the early course of C. rodentium pathogenesis. Histologic changes in the liver correlated with serum elevation of liver transaminases, systemic and liver resident cytokines, as well as signal transduction changes prior to peak bacterial colonization and colonic disease. C. rodentium infection in C57BL/6 mice provides a potentially useful model to study acute liver injury and inflammatory stress under conditions of gastrointestinal infection analogous to enteropathogenic E. coli infection in humans. |
| first_indexed | 2024-09-23T13:06:50Z |
| format | Article |
| id | mit-1721.1/70940 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T13:06:50Z |
| publishDate | 2012 |
| publisher | Public Library of Science |
| record_format | dspace |
| spelling | mit-1721.1/709402022-10-01T13:08:27Z Enteric Infection with Citrobacter rodentium Induces Coagulative Liver Necrosis and Hepatic Inflammation Prior to Peak Infection and Colonic Disease Raczynski, Arkadiusz R. Muthupalani, Sureshkumar Schlieper, Katherine Ann Fox, James G. Tannenbaum, Steven Robert Schauer, David B. Massachusetts Institute of Technology. Department of Biological Engineering Massachusetts Institute of Technology. Division of Comparative Medicine Tannenbaum, Steven Robert Raczynski, Arkadiusz R. Muthupalani, Sureshkumar Schlieper, Katherine Ann Fox, James G. Tannenbaum, Steven Robert Schauer, David B. Acute and chronic forms of inflammation are known to affect liver responses and susceptibility to disease and injury. Furthermore, intestinal microbiota has been shown critical in mediating inflammatory host responses in various animal models. Using C. rodentium, a known enteric bacterial pathogen, we examined liver responses to gastrointestinal infection at various stages of disease pathogenesis. For the first time, to our knowledge, we show distinct liver pathology associated with enteric infection with C. rodentium in C57BL/6 mice, characterized by increased inflammation and hepatitis index scores as well as prominent periportal hepatocellular coagulative necrosis indicative of thrombotic ischemic injury in a subset of animals during the early course of C. rodentium pathogenesis. Histologic changes in the liver correlated with serum elevation of liver transaminases, systemic and liver resident cytokines, as well as signal transduction changes prior to peak bacterial colonization and colonic disease. C. rodentium infection in C57BL/6 mice provides a potentially useful model to study acute liver injury and inflammatory stress under conditions of gastrointestinal infection analogous to enteropathogenic E. coli infection in humans. United States. Army Research Office (Institute for Soldier Nanotechnology grant 6915539 (SRT)) National Institutes of Health (U.S.) (Grant P01 CA026731) National Institutes of Health (U.S.) (Grant P30 ES02109) National Institutes of Health (U.S.) (Toxicology Training grant ES-070220) 2012-05-25T15:25:41Z 2012-05-25T15:25:41Z 2012-03 2012-01 Article http://purl.org/eprint/type/JournalArticle 1932-6203 http://hdl.handle.net/1721.1/70940 Raczynski, Arkadiusz R. et al. “Enteric Infection with Citrobacter Rodentium Induces Coagulative Liver Necrosis and Hepatic Inflammation Prior to Peak Infection and Colonic Disease.” Ed. Bernhard Ryffel. PLoS ONE 7.3 (2012): e33099. Web. https://orcid.org/0000-0001-9307-6116 en_US http://dx.doi.org/10.1371/journal.pone.0033099 PLoS ONE Creative Commons Attribution http://creativecommons.org/licenses/by/2.5/ application/pdf Public Library of Science PLoS |
| spellingShingle | Raczynski, Arkadiusz R. Muthupalani, Sureshkumar Schlieper, Katherine Ann Fox, James G. Tannenbaum, Steven Robert Schauer, David B. Enteric Infection with Citrobacter rodentium Induces Coagulative Liver Necrosis and Hepatic Inflammation Prior to Peak Infection and Colonic Disease |
| title | Enteric Infection with Citrobacter rodentium Induces Coagulative Liver Necrosis and Hepatic Inflammation Prior to Peak Infection and Colonic Disease |
| title_full | Enteric Infection with Citrobacter rodentium Induces Coagulative Liver Necrosis and Hepatic Inflammation Prior to Peak Infection and Colonic Disease |
| title_fullStr | Enteric Infection with Citrobacter rodentium Induces Coagulative Liver Necrosis and Hepatic Inflammation Prior to Peak Infection and Colonic Disease |
| title_full_unstemmed | Enteric Infection with Citrobacter rodentium Induces Coagulative Liver Necrosis and Hepatic Inflammation Prior to Peak Infection and Colonic Disease |
| title_short | Enteric Infection with Citrobacter rodentium Induces Coagulative Liver Necrosis and Hepatic Inflammation Prior to Peak Infection and Colonic Disease |
| title_sort | enteric infection with citrobacter rodentium induces coagulative liver necrosis and hepatic inflammation prior to peak infection and colonic disease |
| url | http://hdl.handle.net/1721.1/70940 https://orcid.org/0000-0001-9307-6116 |
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