Characterization of DNA-binding activity of Zα domains from poxviruses and the importance of the β-wing regions in converting B-DNA to Z-DNA
The E3L gene is essential for pathogenesis in vaccinia virus. The E3L gene product consists of an N-terminal Zα domain and a C-terminal double-stranded RNA (dsRNA) binding domain; the left-handed Z-DNA-binding activity of the Zα domain of E3L is required for viral pathogenicity in mice. E3L is highl...
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| Format: | Article |
| Language: | en_US |
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Oxford University Press (OUP)
2012
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| Online Access: | http://hdl.handle.net/1721.1/70997 https://orcid.org/0000-0002-8187-6498 |
| _version_ | 1811087911406272512 |
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| author | Quyen, Dong Van Ha, Sung Chul Kim, Kyeong Kyu Kim, Yang-Gyun Lowenhaupt, Ky Rich, Alexander |
| author2 | Massachusetts Institute of Technology. Department of Biology |
| author_facet | Massachusetts Institute of Technology. Department of Biology Quyen, Dong Van Ha, Sung Chul Kim, Kyeong Kyu Kim, Yang-Gyun Lowenhaupt, Ky Rich, Alexander |
| author_sort | Quyen, Dong Van |
| collection | MIT |
| description | The E3L gene is essential for pathogenesis in vaccinia virus. The E3L gene product consists of an N-terminal Zα domain and a C-terminal double-stranded RNA (dsRNA) binding domain; the left-handed Z-DNA-binding activity of the Zα domain of E3L is required for viral pathogenicity in mice. E3L is highly conserved among poxviruses, including the smallpox virus, and it is likely that the orthologous Zα domains play similar roles. To better understand the biological function of E3L proteins, we have investigated the Z-DNA-binding behavior of five representative Zα domains from poxviruses. Using surface plasmon resonance (SPR), we have demonstrated that these viral Zα domains bind Z-DNA tightly. Ability of Zα[subscript E3L] converting B-DNA to Z-DNA was measured by circular dichroism (CD). The extents to which these Zαs can stabilize Z-DNA vary considerably. Mutational studies demonstrate that residues in the loop of the β-wing play an important role in this stabilization. Notably the Zα domain of vaccinia E3L acquires ability to convert B-DNA to Z-DNA by mutating amino acid residues in this region. Differences in the host cells of the various poxviruses may require different abilities to stabilize Z-DNA; this may be reflected in the observed differences in behavior in these Zα proteins. |
| first_indexed | 2024-09-23T13:53:51Z |
| format | Article |
| id | mit-1721.1/70997 |
| institution | Massachusetts Institute of Technology |
| language | en_US |
| last_indexed | 2024-09-23T13:53:51Z |
| publishDate | 2012 |
| publisher | Oxford University Press (OUP) |
| record_format | dspace |
| spelling | mit-1721.1/709972022-10-01T17:51:29Z Characterization of DNA-binding activity of Zα domains from poxviruses and the importance of the β-wing regions in converting B-DNA to Z-DNA Quyen, Dong Van Ha, Sung Chul Kim, Kyeong Kyu Kim, Yang-Gyun Lowenhaupt, Ky Rich, Alexander Massachusetts Institute of Technology. Department of Biology Rich, Alexander Lowenhaupt, Ky Rich, Alexander The E3L gene is essential for pathogenesis in vaccinia virus. The E3L gene product consists of an N-terminal Zα domain and a C-terminal double-stranded RNA (dsRNA) binding domain; the left-handed Z-DNA-binding activity of the Zα domain of E3L is required for viral pathogenicity in mice. E3L is highly conserved among poxviruses, including the smallpox virus, and it is likely that the orthologous Zα domains play similar roles. To better understand the biological function of E3L proteins, we have investigated the Z-DNA-binding behavior of five representative Zα domains from poxviruses. Using surface plasmon resonance (SPR), we have demonstrated that these viral Zα domains bind Z-DNA tightly. Ability of Zα[subscript E3L] converting B-DNA to Z-DNA was measured by circular dichroism (CD). The extents to which these Zαs can stabilize Z-DNA vary considerably. Mutational studies demonstrate that residues in the loop of the β-wing play an important role in this stabilization. Notably the Zα domain of vaccinia E3L acquires ability to convert B-DNA to Z-DNA by mutating amino acid residues in this region. Differences in the host cells of the various poxviruses may require different abilities to stabilize Z-DNA; this may be reflected in the observed differences in behavior in these Zα proteins. Korean Science and Engineering Foundation (National Research Laboratory Program (NRL-2006-02287)) Korean Science and Engineering Foundation (Ubiquitome Research Program (M10533010002-06N3301-00210)) Korean Science and Engineering Foundation (21C Frontier Functional Proteomics Program (FPR06B2-120)) National Institutes of Health (U.S.) Ellison Medical Foundation Korea (South). Ministry of Science and Technology (National Laboratory program (NRL-2006-02287)) 2012-06-01T19:57:28Z 2012-06-01T19:57:28Z 2007-11 2007-09 Article http://purl.org/eprint/type/JournalArticle 0305-1048 1362-4962 http://hdl.handle.net/1721.1/70997 Van Quyen, D. et al. “Characterization of DNA-binding Activity of Z Domains from Poxviruses and the Importance of the -wing Regions in Converting B-DNA to Z-DNA.” Nucleic Acids Research 35.22 (2007): 7714–7720. Web. 1 June 2012. https://orcid.org/0000-0002-8187-6498 en_US http://dx.doi.org/10.1093/nar/gkm748 Nucleic Acids Research Creative Commons Attribution Non-Commercial http://creativecommons.org/licenses/by-nc/2.5 application/pdf Oxford University Press (OUP) Oxford |
| spellingShingle | Quyen, Dong Van Ha, Sung Chul Kim, Kyeong Kyu Kim, Yang-Gyun Lowenhaupt, Ky Rich, Alexander Characterization of DNA-binding activity of Zα domains from poxviruses and the importance of the β-wing regions in converting B-DNA to Z-DNA |
| title | Characterization of DNA-binding activity of Zα domains from poxviruses and the importance of the β-wing regions in converting B-DNA to Z-DNA |
| title_full | Characterization of DNA-binding activity of Zα domains from poxviruses and the importance of the β-wing regions in converting B-DNA to Z-DNA |
| title_fullStr | Characterization of DNA-binding activity of Zα domains from poxviruses and the importance of the β-wing regions in converting B-DNA to Z-DNA |
| title_full_unstemmed | Characterization of DNA-binding activity of Zα domains from poxviruses and the importance of the β-wing regions in converting B-DNA to Z-DNA |
| title_short | Characterization of DNA-binding activity of Zα domains from poxviruses and the importance of the β-wing regions in converting B-DNA to Z-DNA |
| title_sort | characterization of dna binding activity of zα domains from poxviruses and the importance of the β wing regions in converting b dna to z dna |
| url | http://hdl.handle.net/1721.1/70997 https://orcid.org/0000-0002-8187-6498 |
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